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Blueberry Anthocyanins-Enriched Extracts Attenuate Cyclophosphamide-Induced Cardiac Injury.

Liu Y, Tan D, Shi L, Liu X, Zhang Y, Tong C, Song D, Hou M - PLoS ONE (2015)

Bottom Line: Four weeks 20mg/kg and 80mg/kg dose of BAE treatment following CTX exposure attenuated mean arterial blood pressure, heart rate and activities of heart enzymes, improved cardiac dysfunction, left ventricular hypertrophy and fibrosis.Importantly, BAE also attenuated CTX-induced LV leukocyte infiltration and inflammatory cytokines expression, ameliorated oxidative stress as well as cardiomyocyte apoptosis.In conclusion, BAE attenuated the CTX-induced cardiac injury and the protective mechanisms were related closely to the anti-inflammatory, antioxidant and anti-inflammatory characteristics of BAE.

View Article: PubMed Central - PubMed

Affiliation: Emergency Medicine Department of General Hospital of Shenyang Military Command, Laboratory of Rescue Center of Severe Wound and Trauma PLA, Shenyang, China.

ABSTRACT
We sought to explore the effect of blueberry anthocyanins-enriched extracts (BAE) on cyclophosphamide (CTX)-induced cardiac injury. The rats were divided randomly into five groups including normal control, CTX 100 mg/kg, BAE 80mg/kg, CTX+BAE 20mg/kg and CTX+BAE 80mg/kg groups. The rats in the three BAE-treated groups were administered BAE for four weeks. Seven days after BAE administration, rats in CTX group and two BAE-treated groups were intraperitoneally injected with a single dose of 100 mg/kg CTX. Cardiac injury was assessed using physiological parameters, Echo, morphological staining, real-time PCR and western blot. In addition, cardiotoxicity indices, inflammatory cytokines expression and oxidative stress markers were also detected. Four weeks 20mg/kg and 80mg/kg dose of BAE treatment following CTX exposure attenuated mean arterial blood pressure, heart rate and activities of heart enzymes, improved cardiac dysfunction, left ventricular hypertrophy and fibrosis. Importantly, BAE also attenuated CTX-induced LV leukocyte infiltration and inflammatory cytokines expression, ameliorated oxidative stress as well as cardiomyocyte apoptosis. In conclusion, BAE attenuated the CTX-induced cardiac injury and the protective mechanisms were related closely to the anti-inflammatory, antioxidant and anti-inflammatory characteristics of BAE.

No MeSH data available.


Related in: MedlinePlus

BAE attenuated CTX-induced oxidative stress.Heart tissues were performed to detect the levels of MDA(A), SOD(B) and GSH(C). Data are mean ± SD. # p < 0.05, compared to normal control group; * p < 0.05, compared to CTX.
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pone.0127813.g007: BAE attenuated CTX-induced oxidative stress.Heart tissues were performed to detect the levels of MDA(A), SOD(B) and GSH(C). Data are mean ± SD. # p < 0.05, compared to normal control group; * p < 0.05, compared to CTX.

Mentions: To assess the effect of BAE on CTX-induced oxidative stress, we assessed MDA levels (a marker of oxidative stress), SOD and GSH activity (antioxidant status marker). The results demonstrated the highest MDA level in the CTX group among all the groups. 20 and 80 mg/kg BAE significantly inhibited the increase in MDA level. In addition, rats in the CTX group showed the lowest SOD and GSH activity, while BAE significantly antagonized the decrease in SOD and GSH activity induced by CTX (Fig 7A–7C, p< 0.05).


Blueberry Anthocyanins-Enriched Extracts Attenuate Cyclophosphamide-Induced Cardiac Injury.

Liu Y, Tan D, Shi L, Liu X, Zhang Y, Tong C, Song D, Hou M - PLoS ONE (2015)

BAE attenuated CTX-induced oxidative stress.Heart tissues were performed to detect the levels of MDA(A), SOD(B) and GSH(C). Data are mean ± SD. # p < 0.05, compared to normal control group; * p < 0.05, compared to CTX.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4489910&req=5

pone.0127813.g007: BAE attenuated CTX-induced oxidative stress.Heart tissues were performed to detect the levels of MDA(A), SOD(B) and GSH(C). Data are mean ± SD. # p < 0.05, compared to normal control group; * p < 0.05, compared to CTX.
Mentions: To assess the effect of BAE on CTX-induced oxidative stress, we assessed MDA levels (a marker of oxidative stress), SOD and GSH activity (antioxidant status marker). The results demonstrated the highest MDA level in the CTX group among all the groups. 20 and 80 mg/kg BAE significantly inhibited the increase in MDA level. In addition, rats in the CTX group showed the lowest SOD and GSH activity, while BAE significantly antagonized the decrease in SOD and GSH activity induced by CTX (Fig 7A–7C, p< 0.05).

Bottom Line: Four weeks 20mg/kg and 80mg/kg dose of BAE treatment following CTX exposure attenuated mean arterial blood pressure, heart rate and activities of heart enzymes, improved cardiac dysfunction, left ventricular hypertrophy and fibrosis.Importantly, BAE also attenuated CTX-induced LV leukocyte infiltration and inflammatory cytokines expression, ameliorated oxidative stress as well as cardiomyocyte apoptosis.In conclusion, BAE attenuated the CTX-induced cardiac injury and the protective mechanisms were related closely to the anti-inflammatory, antioxidant and anti-inflammatory characteristics of BAE.

View Article: PubMed Central - PubMed

Affiliation: Emergency Medicine Department of General Hospital of Shenyang Military Command, Laboratory of Rescue Center of Severe Wound and Trauma PLA, Shenyang, China.

ABSTRACT
We sought to explore the effect of blueberry anthocyanins-enriched extracts (BAE) on cyclophosphamide (CTX)-induced cardiac injury. The rats were divided randomly into five groups including normal control, CTX 100 mg/kg, BAE 80mg/kg, CTX+BAE 20mg/kg and CTX+BAE 80mg/kg groups. The rats in the three BAE-treated groups were administered BAE for four weeks. Seven days after BAE administration, rats in CTX group and two BAE-treated groups were intraperitoneally injected with a single dose of 100 mg/kg CTX. Cardiac injury was assessed using physiological parameters, Echo, morphological staining, real-time PCR and western blot. In addition, cardiotoxicity indices, inflammatory cytokines expression and oxidative stress markers were also detected. Four weeks 20mg/kg and 80mg/kg dose of BAE treatment following CTX exposure attenuated mean arterial blood pressure, heart rate and activities of heart enzymes, improved cardiac dysfunction, left ventricular hypertrophy and fibrosis. Importantly, BAE also attenuated CTX-induced LV leukocyte infiltration and inflammatory cytokines expression, ameliorated oxidative stress as well as cardiomyocyte apoptosis. In conclusion, BAE attenuated the CTX-induced cardiac injury and the protective mechanisms were related closely to the anti-inflammatory, antioxidant and anti-inflammatory characteristics of BAE.

No MeSH data available.


Related in: MedlinePlus