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Blueberry Anthocyanins-Enriched Extracts Attenuate Cyclophosphamide-Induced Cardiac Injury.

Liu Y, Tan D, Shi L, Liu X, Zhang Y, Tong C, Song D, Hou M - PLoS ONE (2015)

Bottom Line: Four weeks 20mg/kg and 80mg/kg dose of BAE treatment following CTX exposure attenuated mean arterial blood pressure, heart rate and activities of heart enzymes, improved cardiac dysfunction, left ventricular hypertrophy and fibrosis.Importantly, BAE also attenuated CTX-induced LV leukocyte infiltration and inflammatory cytokines expression, ameliorated oxidative stress as well as cardiomyocyte apoptosis.In conclusion, BAE attenuated the CTX-induced cardiac injury and the protective mechanisms were related closely to the anti-inflammatory, antioxidant and anti-inflammatory characteristics of BAE.

View Article: PubMed Central - PubMed

Affiliation: Emergency Medicine Department of General Hospital of Shenyang Military Command, Laboratory of Rescue Center of Severe Wound and Trauma PLA, Shenyang, China.

ABSTRACT
We sought to explore the effect of blueberry anthocyanins-enriched extracts (BAE) on cyclophosphamide (CTX)-induced cardiac injury. The rats were divided randomly into five groups including normal control, CTX 100 mg/kg, BAE 80mg/kg, CTX+BAE 20mg/kg and CTX+BAE 80mg/kg groups. The rats in the three BAE-treated groups were administered BAE for four weeks. Seven days after BAE administration, rats in CTX group and two BAE-treated groups were intraperitoneally injected with a single dose of 100 mg/kg CTX. Cardiac injury was assessed using physiological parameters, Echo, morphological staining, real-time PCR and western blot. In addition, cardiotoxicity indices, inflammatory cytokines expression and oxidative stress markers were also detected. Four weeks 20mg/kg and 80mg/kg dose of BAE treatment following CTX exposure attenuated mean arterial blood pressure, heart rate and activities of heart enzymes, improved cardiac dysfunction, left ventricular hypertrophy and fibrosis. Importantly, BAE also attenuated CTX-induced LV leukocyte infiltration and inflammatory cytokines expression, ameliorated oxidative stress as well as cardiomyocyte apoptosis. In conclusion, BAE attenuated the CTX-induced cardiac injury and the protective mechanisms were related closely to the anti-inflammatory, antioxidant and anti-inflammatory characteristics of BAE.

No MeSH data available.


Related in: MedlinePlus

BAE attenuated CTX-induced LV inflammation.A and F: Normal control; B and G: BAE80mg/kg; C and H: CTX group; D and I: CTX+BAE20mg/kg; E and J: CTX+BAE80mg/kg. (A-E) show the histopathological changes in CTX-induced cardiomyocyte. The black arrow in C and D represents respectively inflammatory cells infiltration and heart congestion, whereas the brown arrow represents cardiomyocyte apoptosis (HE staining, Bar: 20 μm). (F-J) demonstrate the infiltration of CD45-positive leukocytes in the left ventricle of the rats in four groups. The arrow in H represents the CD45-positive leukocytes (immunohistochemical staining, Bar: 20 μm). (K) Bar charts show a number of CD45-positive leukocytes in the myocardial interstitium. Data are mean ± SD. #p < 0.05, compared to normal control group; *p < 0.05, compared to CTX.
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pone.0127813.g005: BAE attenuated CTX-induced LV inflammation.A and F: Normal control; B and G: BAE80mg/kg; C and H: CTX group; D and I: CTX+BAE20mg/kg; E and J: CTX+BAE80mg/kg. (A-E) show the histopathological changes in CTX-induced cardiomyocyte. The black arrow in C and D represents respectively inflammatory cells infiltration and heart congestion, whereas the brown arrow represents cardiomyocyte apoptosis (HE staining, Bar: 20 μm). (F-J) demonstrate the infiltration of CD45-positive leukocytes in the left ventricle of the rats in four groups. The arrow in H represents the CD45-positive leukocytes (immunohistochemical staining, Bar: 20 μm). (K) Bar charts show a number of CD45-positive leukocytes in the myocardial interstitium. Data are mean ± SD. #p < 0.05, compared to normal control group; *p < 0.05, compared to CTX.

Mentions: No heart injury was observed in normal control group animals. In contrast, extensive heart injury was observed in animals from the CTX group, there was obvious cardiac hypertrophy, heart edema with inflammatory cell infiltration. BAE attenuated the histopathological changes induced by CTX, especially in the 80 mg/kg group, with less myocardial leukocyte infiltration (Fig 5A–5E). To assess the effects of BAE on myocardial leukocyte infiltration, we investigated LV leukocyte infiltration density using CD45 immunohistochemical staining. There was a significant increase in the number of CD45-positive in CTX group compared with in the normal control group. However, there was significant decreased of leukocyte infiltration density in the BAE 20mg/kg and BAE80mg/kg groups compared with the CTX group (Fig 5F–5K, p< 0.05).


Blueberry Anthocyanins-Enriched Extracts Attenuate Cyclophosphamide-Induced Cardiac Injury.

Liu Y, Tan D, Shi L, Liu X, Zhang Y, Tong C, Song D, Hou M - PLoS ONE (2015)

BAE attenuated CTX-induced LV inflammation.A and F: Normal control; B and G: BAE80mg/kg; C and H: CTX group; D and I: CTX+BAE20mg/kg; E and J: CTX+BAE80mg/kg. (A-E) show the histopathological changes in CTX-induced cardiomyocyte. The black arrow in C and D represents respectively inflammatory cells infiltration and heart congestion, whereas the brown arrow represents cardiomyocyte apoptosis (HE staining, Bar: 20 μm). (F-J) demonstrate the infiltration of CD45-positive leukocytes in the left ventricle of the rats in four groups. The arrow in H represents the CD45-positive leukocytes (immunohistochemical staining, Bar: 20 μm). (K) Bar charts show a number of CD45-positive leukocytes in the myocardial interstitium. Data are mean ± SD. #p < 0.05, compared to normal control group; *p < 0.05, compared to CTX.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4489910&req=5

pone.0127813.g005: BAE attenuated CTX-induced LV inflammation.A and F: Normal control; B and G: BAE80mg/kg; C and H: CTX group; D and I: CTX+BAE20mg/kg; E and J: CTX+BAE80mg/kg. (A-E) show the histopathological changes in CTX-induced cardiomyocyte. The black arrow in C and D represents respectively inflammatory cells infiltration and heart congestion, whereas the brown arrow represents cardiomyocyte apoptosis (HE staining, Bar: 20 μm). (F-J) demonstrate the infiltration of CD45-positive leukocytes in the left ventricle of the rats in four groups. The arrow in H represents the CD45-positive leukocytes (immunohistochemical staining, Bar: 20 μm). (K) Bar charts show a number of CD45-positive leukocytes in the myocardial interstitium. Data are mean ± SD. #p < 0.05, compared to normal control group; *p < 0.05, compared to CTX.
Mentions: No heart injury was observed in normal control group animals. In contrast, extensive heart injury was observed in animals from the CTX group, there was obvious cardiac hypertrophy, heart edema with inflammatory cell infiltration. BAE attenuated the histopathological changes induced by CTX, especially in the 80 mg/kg group, with less myocardial leukocyte infiltration (Fig 5A–5E). To assess the effects of BAE on myocardial leukocyte infiltration, we investigated LV leukocyte infiltration density using CD45 immunohistochemical staining. There was a significant increase in the number of CD45-positive in CTX group compared with in the normal control group. However, there was significant decreased of leukocyte infiltration density in the BAE 20mg/kg and BAE80mg/kg groups compared with the CTX group (Fig 5F–5K, p< 0.05).

Bottom Line: Four weeks 20mg/kg and 80mg/kg dose of BAE treatment following CTX exposure attenuated mean arterial blood pressure, heart rate and activities of heart enzymes, improved cardiac dysfunction, left ventricular hypertrophy and fibrosis.Importantly, BAE also attenuated CTX-induced LV leukocyte infiltration and inflammatory cytokines expression, ameliorated oxidative stress as well as cardiomyocyte apoptosis.In conclusion, BAE attenuated the CTX-induced cardiac injury and the protective mechanisms were related closely to the anti-inflammatory, antioxidant and anti-inflammatory characteristics of BAE.

View Article: PubMed Central - PubMed

Affiliation: Emergency Medicine Department of General Hospital of Shenyang Military Command, Laboratory of Rescue Center of Severe Wound and Trauma PLA, Shenyang, China.

ABSTRACT
We sought to explore the effect of blueberry anthocyanins-enriched extracts (BAE) on cyclophosphamide (CTX)-induced cardiac injury. The rats were divided randomly into five groups including normal control, CTX 100 mg/kg, BAE 80mg/kg, CTX+BAE 20mg/kg and CTX+BAE 80mg/kg groups. The rats in the three BAE-treated groups were administered BAE for four weeks. Seven days after BAE administration, rats in CTX group and two BAE-treated groups were intraperitoneally injected with a single dose of 100 mg/kg CTX. Cardiac injury was assessed using physiological parameters, Echo, morphological staining, real-time PCR and western blot. In addition, cardiotoxicity indices, inflammatory cytokines expression and oxidative stress markers were also detected. Four weeks 20mg/kg and 80mg/kg dose of BAE treatment following CTX exposure attenuated mean arterial blood pressure, heart rate and activities of heart enzymes, improved cardiac dysfunction, left ventricular hypertrophy and fibrosis. Importantly, BAE also attenuated CTX-induced LV leukocyte infiltration and inflammatory cytokines expression, ameliorated oxidative stress as well as cardiomyocyte apoptosis. In conclusion, BAE attenuated the CTX-induced cardiac injury and the protective mechanisms were related closely to the anti-inflammatory, antioxidant and anti-inflammatory characteristics of BAE.

No MeSH data available.


Related in: MedlinePlus