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Antioxidant Properties of Whole Body Periodic Acceleration (pGz).

Uryash A, Bassuk J, Kurlansky P, Altamirano F, Lopez JR, Adams JA - PLoS ONE (2015)

Bottom Line: Application of pGz was associated with significantly increased expression of endogenous antioxidants (Glutathioneperoxidase-1(GPX-1), Catalase (CAT), Superoxide, Superoxide Dismutase 1(SOD1).This led to an increase of total cardiac antioxidant capacity along with an increase in the antioxidant response element transcription factor Nrf2 translocation to the nucleus. pGz decreased reactive oxygen species in both mice models of oxidative stress.Thus, pGz is a novel non-pharmacologic method to harness endogenous antioxidant capacity.

View Article: PubMed Central - PubMed

Affiliation: Division of Neonatology, Mount Sinai Medical Center, Miami Beach, Florida, United States of America.

ABSTRACT
The recognition that oxidative stress is a major component of several chronic diseases has engendered numerous trials of antioxidant therapies with minimal or no direct benefits. Nanomolar quantities of nitric oxide released into the circulation by pharmacologic stimulation of eNOS have antioxidant properties but physiologic stimulation as through increased pulsatile shear stress of the endothelium has not been assessed. The present study utilized a non-invasive technology, periodic acceleration (pGz) that increases pulsatile shear stress such that upregulation of cardiac eNOS occurs, We assessed its efficacy in normal mice and mouse models with high levels of oxidative stress, e.g. Diabetes type 1 and mdx (Duchene Muscular Dystrophy). pGz increased protein expression and upregulated eNOS in hearts. Application of pGz was associated with significantly increased expression of endogenous antioxidants (Glutathioneperoxidase-1(GPX-1), Catalase (CAT), Superoxide, Superoxide Dismutase 1(SOD1). This led to an increase of total cardiac antioxidant capacity along with an increase in the antioxidant response element transcription factor Nrf2 translocation to the nucleus. pGz decreased reactive oxygen species in both mice models of oxidative stress. Thus, pGz is a novel non-pharmacologic method to harness endogenous antioxidant capacity.

No MeSH data available.


Related in: MedlinePlus

Antioxidant Protein Expression is Induced by pGz.This figure depicts the effects of 1, 2, and 4 wk. of daily pGz in normal mice on the expressions of (A). Glutathioneperoxidase-1 (GPX1), (B). Catalase (CAT), and (C). Superoxide Dismutase 1 (SOD1). The relative protein expressions of GPX1, CAT and SOD1 over time compared to time of CONT values showed that pGz significantly increased GPX1, CAT, and SOD1 expression at 1, 2 and 4 wk compared to time CONT (‡p < 0.01 1, 2, and 4 wk. pGz vs. CONT). One, 2, and 4 wk. of pGz significantly increased expression of GPX1, CAT, and SOD1 compared to baseline (BL) values for both CONT and pGz groups (* p< 0.01 1,2, and 4 wk. vs. BL values). Representative Immunoblots of protein expression of GPX1, CAT, SOD1 and protein quantity loading Glyceraldehyde 3-phosphate dehydrogenase (GADPH) after 4 wk. of pGz, for CONT and pGz groups showing significant expression of these compared to time CONT.
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pone.0131392.g001: Antioxidant Protein Expression is Induced by pGz.This figure depicts the effects of 1, 2, and 4 wk. of daily pGz in normal mice on the expressions of (A). Glutathioneperoxidase-1 (GPX1), (B). Catalase (CAT), and (C). Superoxide Dismutase 1 (SOD1). The relative protein expressions of GPX1, CAT and SOD1 over time compared to time of CONT values showed that pGz significantly increased GPX1, CAT, and SOD1 expression at 1, 2 and 4 wk compared to time CONT (‡p < 0.01 1, 2, and 4 wk. pGz vs. CONT). One, 2, and 4 wk. of pGz significantly increased expression of GPX1, CAT, and SOD1 compared to baseline (BL) values for both CONT and pGz groups (* p< 0.01 1,2, and 4 wk. vs. BL values). Representative Immunoblots of protein expression of GPX1, CAT, SOD1 and protein quantity loading Glyceraldehyde 3-phosphate dehydrogenase (GADPH) after 4 wk. of pGz, for CONT and pGz groups showing significant expression of these compared to time CONT.

Mentions: Induction of antioxidant enzymes by pGz was measured after 1, 2 and 4 wk. of daily pGz. Peak effect of enzymatic expression and total antioxidant capacity was seen after 4 wk. of pGz for GPX1, CAT, SOD1 (Figs 1 and 2). After 4 wk. of pGz, protein expression and upregulation of the antioxidant response element, transcription factor Nrf2, was significantly increased. Furthermore, pGz induced Nrf2 translocation from cytosol to nucleus (Fig 3).


Antioxidant Properties of Whole Body Periodic Acceleration (pGz).

Uryash A, Bassuk J, Kurlansky P, Altamirano F, Lopez JR, Adams JA - PLoS ONE (2015)

Antioxidant Protein Expression is Induced by pGz.This figure depicts the effects of 1, 2, and 4 wk. of daily pGz in normal mice on the expressions of (A). Glutathioneperoxidase-1 (GPX1), (B). Catalase (CAT), and (C). Superoxide Dismutase 1 (SOD1). The relative protein expressions of GPX1, CAT and SOD1 over time compared to time of CONT values showed that pGz significantly increased GPX1, CAT, and SOD1 expression at 1, 2 and 4 wk compared to time CONT (‡p < 0.01 1, 2, and 4 wk. pGz vs. CONT). One, 2, and 4 wk. of pGz significantly increased expression of GPX1, CAT, and SOD1 compared to baseline (BL) values for both CONT and pGz groups (* p< 0.01 1,2, and 4 wk. vs. BL values). Representative Immunoblots of protein expression of GPX1, CAT, SOD1 and protein quantity loading Glyceraldehyde 3-phosphate dehydrogenase (GADPH) after 4 wk. of pGz, for CONT and pGz groups showing significant expression of these compared to time CONT.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4489838&req=5

pone.0131392.g001: Antioxidant Protein Expression is Induced by pGz.This figure depicts the effects of 1, 2, and 4 wk. of daily pGz in normal mice on the expressions of (A). Glutathioneperoxidase-1 (GPX1), (B). Catalase (CAT), and (C). Superoxide Dismutase 1 (SOD1). The relative protein expressions of GPX1, CAT and SOD1 over time compared to time of CONT values showed that pGz significantly increased GPX1, CAT, and SOD1 expression at 1, 2 and 4 wk compared to time CONT (‡p < 0.01 1, 2, and 4 wk. pGz vs. CONT). One, 2, and 4 wk. of pGz significantly increased expression of GPX1, CAT, and SOD1 compared to baseline (BL) values for both CONT and pGz groups (* p< 0.01 1,2, and 4 wk. vs. BL values). Representative Immunoblots of protein expression of GPX1, CAT, SOD1 and protein quantity loading Glyceraldehyde 3-phosphate dehydrogenase (GADPH) after 4 wk. of pGz, for CONT and pGz groups showing significant expression of these compared to time CONT.
Mentions: Induction of antioxidant enzymes by pGz was measured after 1, 2 and 4 wk. of daily pGz. Peak effect of enzymatic expression and total antioxidant capacity was seen after 4 wk. of pGz for GPX1, CAT, SOD1 (Figs 1 and 2). After 4 wk. of pGz, protein expression and upregulation of the antioxidant response element, transcription factor Nrf2, was significantly increased. Furthermore, pGz induced Nrf2 translocation from cytosol to nucleus (Fig 3).

Bottom Line: Application of pGz was associated with significantly increased expression of endogenous antioxidants (Glutathioneperoxidase-1(GPX-1), Catalase (CAT), Superoxide, Superoxide Dismutase 1(SOD1).This led to an increase of total cardiac antioxidant capacity along with an increase in the antioxidant response element transcription factor Nrf2 translocation to the nucleus. pGz decreased reactive oxygen species in both mice models of oxidative stress.Thus, pGz is a novel non-pharmacologic method to harness endogenous antioxidant capacity.

View Article: PubMed Central - PubMed

Affiliation: Division of Neonatology, Mount Sinai Medical Center, Miami Beach, Florida, United States of America.

ABSTRACT
The recognition that oxidative stress is a major component of several chronic diseases has engendered numerous trials of antioxidant therapies with minimal or no direct benefits. Nanomolar quantities of nitric oxide released into the circulation by pharmacologic stimulation of eNOS have antioxidant properties but physiologic stimulation as through increased pulsatile shear stress of the endothelium has not been assessed. The present study utilized a non-invasive technology, periodic acceleration (pGz) that increases pulsatile shear stress such that upregulation of cardiac eNOS occurs, We assessed its efficacy in normal mice and mouse models with high levels of oxidative stress, e.g. Diabetes type 1 and mdx (Duchene Muscular Dystrophy). pGz increased protein expression and upregulated eNOS in hearts. Application of pGz was associated with significantly increased expression of endogenous antioxidants (Glutathioneperoxidase-1(GPX-1), Catalase (CAT), Superoxide, Superoxide Dismutase 1(SOD1). This led to an increase of total cardiac antioxidant capacity along with an increase in the antioxidant response element transcription factor Nrf2 translocation to the nucleus. pGz decreased reactive oxygen species in both mice models of oxidative stress. Thus, pGz is a novel non-pharmacologic method to harness endogenous antioxidant capacity.

No MeSH data available.


Related in: MedlinePlus