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Interference and Mechanism of Dill Seed Essential Oil and Contribution of Carvone and Limonene in Preventing Sclerotinia Rot of Rapeseed.

Ma B, Ban X, Huang B, He J, Tian J, Zeng H, Chen Y, Wang Y - PLoS ONE (2015)

Bottom Line: This study aimed to evaluate the inhibitory effects of dill (Anethum graveolens L.) seed essential oil against Sclerotinia sclerotiorum and its mechanism of action.Carvone and limonene synergistically inhibited the growth of the fungus.In vivo experiments, the essential oil remarkably suppressed S. sclerotiorum, and considerable morphological alterations were observed in the hyphae and sclerotia.

View Article: PubMed Central - PubMed

Affiliation: Institute of TCM and Natural Products, School of Pharmaceutical Sciences, Wuhan University, Wuhan, P.R. China.

ABSTRACT
This study aimed to evaluate the inhibitory effects of dill (Anethum graveolens L.) seed essential oil against Sclerotinia sclerotiorum and its mechanism of action. The antifungal activities of the two main constituents, namely carvone and limonene, were also measured. Mycelial growth and sclerotial germination were thoroughly inhibited by dill seed essential oil at the 1.00 μL/mL under contact condition and 0.125μL/mL air under vapor condition. Carvone also contributed more than limonene in inhibiting the growth of S. sclerotiorum. Carvone and limonene synergistically inhibited the growth of the fungus. In vivo experiments, the essential oil remarkably suppressed S. sclerotiorum, and considerable morphological alterations were observed in the hyphae and sclerotia. Inhibition of ergosterol synthesis, malate dehydrogenase, succinate dehydrogenase activities, and external medium acidification were investigated to elucidate the antifungal mechanism of the essential oil. The seed essential oil of A. graveolens can be extensively used in agriculture for preventing the oilseed crops fungal disease.

No MeSH data available.


Related in: MedlinePlus

Efficacy of the oil against S. sclerotiorum in potted plants.(A) Control; (B)–(E) Treated with the oil (1.25, 2.50, 5.00, and 10.00 μL/mL); (F) Treated with carbendazol (1.00 mg/mL).
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pone.0131733.g005: Efficacy of the oil against S. sclerotiorum in potted plants.(A) Control; (B)–(E) Treated with the oil (1.25, 2.50, 5.00, and 10.00 μL/mL); (F) Treated with carbendazol (1.00 mg/mL).

Mentions: In potted plant tests, 1.25 μL/mL to 10.00 μL/mL of the oil inhibited Sclerotinia disease development 7 d after inoculation with S. sclerotiorum. Furthermore, the average reduction in disease was 40.78% to 100.00% compared with that of the control (Table 3). The interference of dill seed essential oil was remarkable at the highest concentration compared with carbendazol. By contrast, a moderate interferential effect was the characteristic feature of dill seed essential oil at low concentrations (Fig 5).


Interference and Mechanism of Dill Seed Essential Oil and Contribution of Carvone and Limonene in Preventing Sclerotinia Rot of Rapeseed.

Ma B, Ban X, Huang B, He J, Tian J, Zeng H, Chen Y, Wang Y - PLoS ONE (2015)

Efficacy of the oil against S. sclerotiorum in potted plants.(A) Control; (B)–(E) Treated with the oil (1.25, 2.50, 5.00, and 10.00 μL/mL); (F) Treated with carbendazol (1.00 mg/mL).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4489822&req=5

pone.0131733.g005: Efficacy of the oil against S. sclerotiorum in potted plants.(A) Control; (B)–(E) Treated with the oil (1.25, 2.50, 5.00, and 10.00 μL/mL); (F) Treated with carbendazol (1.00 mg/mL).
Mentions: In potted plant tests, 1.25 μL/mL to 10.00 μL/mL of the oil inhibited Sclerotinia disease development 7 d after inoculation with S. sclerotiorum. Furthermore, the average reduction in disease was 40.78% to 100.00% compared with that of the control (Table 3). The interference of dill seed essential oil was remarkable at the highest concentration compared with carbendazol. By contrast, a moderate interferential effect was the characteristic feature of dill seed essential oil at low concentrations (Fig 5).

Bottom Line: This study aimed to evaluate the inhibitory effects of dill (Anethum graveolens L.) seed essential oil against Sclerotinia sclerotiorum and its mechanism of action.Carvone and limonene synergistically inhibited the growth of the fungus.In vivo experiments, the essential oil remarkably suppressed S. sclerotiorum, and considerable morphological alterations were observed in the hyphae and sclerotia.

View Article: PubMed Central - PubMed

Affiliation: Institute of TCM and Natural Products, School of Pharmaceutical Sciences, Wuhan University, Wuhan, P.R. China.

ABSTRACT
This study aimed to evaluate the inhibitory effects of dill (Anethum graveolens L.) seed essential oil against Sclerotinia sclerotiorum and its mechanism of action. The antifungal activities of the two main constituents, namely carvone and limonene, were also measured. Mycelial growth and sclerotial germination were thoroughly inhibited by dill seed essential oil at the 1.00 μL/mL under contact condition and 0.125μL/mL air under vapor condition. Carvone also contributed more than limonene in inhibiting the growth of S. sclerotiorum. Carvone and limonene synergistically inhibited the growth of the fungus. In vivo experiments, the essential oil remarkably suppressed S. sclerotiorum, and considerable morphological alterations were observed in the hyphae and sclerotia. Inhibition of ergosterol synthesis, malate dehydrogenase, succinate dehydrogenase activities, and external medium acidification were investigated to elucidate the antifungal mechanism of the essential oil. The seed essential oil of A. graveolens can be extensively used in agriculture for preventing the oilseed crops fungal disease.

No MeSH data available.


Related in: MedlinePlus