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Interference and Mechanism of Dill Seed Essential Oil and Contribution of Carvone and Limonene in Preventing Sclerotinia Rot of Rapeseed.

Ma B, Ban X, Huang B, He J, Tian J, Zeng H, Chen Y, Wang Y - PLoS ONE (2015)

Bottom Line: This study aimed to evaluate the inhibitory effects of dill (Anethum graveolens L.) seed essential oil against Sclerotinia sclerotiorum and its mechanism of action.In vivo experiments, the essential oil remarkably suppressed S. sclerotiorum, and considerable morphological alterations were observed in the hyphae and sclerotia.The seed essential oil of A. graveolens can be extensively used in agriculture for preventing the oilseed crops fungal disease.

View Article: PubMed Central - PubMed

Affiliation: Institute of TCM and Natural Products, School of Pharmaceutical Sciences, Wuhan University, Wuhan, P.R. China.

ABSTRACT
This study aimed to evaluate the inhibitory effects of dill (Anethum graveolens L.) seed essential oil against Sclerotinia sclerotiorum and its mechanism of action. The antifungal activities of the two main constituents, namely carvone and limonene, were also measured. Mycelial growth and sclerotial germination were thoroughly inhibited by dill seed essential oil at the 1.00 μL/mL under contact condition and 0.125μL/mL air under vapor condition. Carvone also contributed more than limonene in inhibiting the growth of S. sclerotiorum. Carvone and limonene synergistically inhibited the growth of the fungus. In vivo experiments, the essential oil remarkably suppressed S. sclerotiorum, and considerable morphological alterations were observed in the hyphae and sclerotia. Inhibition of ergosterol synthesis, malate dehydrogenase, succinate dehydrogenase activities, and external medium acidification were investigated to elucidate the antifungal mechanism of the essential oil. The seed essential oil of A. graveolens can be extensively used in agriculture for preventing the oilseed crops fungal disease.

No MeSH data available.


Related in: MedlinePlus

Effects of the samples at vapor phase on colony diameter (cm) growth of S. sclerotiorum.(A) The oil, (B) mixture of carvone and limonene, (C) limonene, (D) carvone. Values are means (n = 3) ± standard deviations.
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pone.0131733.g002: Effects of the samples at vapor phase on colony diameter (cm) growth of S. sclerotiorum.(A) The oil, (B) mixture of carvone and limonene, (C) limonene, (D) carvone. Values are means (n = 3) ± standard deviations.

Mentions: The contact phase effects of different concentrations of the essential oil, carvone, limonene and the mixture of carvone and limonene on mycelial growth of S. sclerotiorum in vitro are shown in Fig 1A, 1B, 1C and 1D. Dill seed essential oil (1.00 μL/mL) completely inhibited fungal growth after 4 d of incubation. The mixture exhibited better activity than both carvone and limonene (Fig 1B). Carvone contributed more than limonene to the inhibitory activity of essential oil (Fig 1C and 1D). The contribution of carvone and limonene was normalized by inhibition percent. The average contributions of carvone and limonene were 77.35% and 19.16%, respectively. The contribution of the mixture was 82.47%. The vapor phase effects of dill seed essential oil, the mixture of carvone and limonene, carvone, and limonene were greater than their contact inhibitory effects on mycelial growth. Results of the vapor phase effects are shown in Fig 2. In the vapor phase test at 0.125 μL/mL essential oil, 0.093 μL/mL the mixture, and 0.052 μL/mL carvone in air, fungal development was completely inhibited after 4 d of incubation. Furthermore, 0.041 μL/mL limonene can delay the growth for 2 d. The inhibitory percent of the mixture is also similar to that of essential oil.


Interference and Mechanism of Dill Seed Essential Oil and Contribution of Carvone and Limonene in Preventing Sclerotinia Rot of Rapeseed.

Ma B, Ban X, Huang B, He J, Tian J, Zeng H, Chen Y, Wang Y - PLoS ONE (2015)

Effects of the samples at vapor phase on colony diameter (cm) growth of S. sclerotiorum.(A) The oil, (B) mixture of carvone and limonene, (C) limonene, (D) carvone. Values are means (n = 3) ± standard deviations.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4489822&req=5

pone.0131733.g002: Effects of the samples at vapor phase on colony diameter (cm) growth of S. sclerotiorum.(A) The oil, (B) mixture of carvone and limonene, (C) limonene, (D) carvone. Values are means (n = 3) ± standard deviations.
Mentions: The contact phase effects of different concentrations of the essential oil, carvone, limonene and the mixture of carvone and limonene on mycelial growth of S. sclerotiorum in vitro are shown in Fig 1A, 1B, 1C and 1D. Dill seed essential oil (1.00 μL/mL) completely inhibited fungal growth after 4 d of incubation. The mixture exhibited better activity than both carvone and limonene (Fig 1B). Carvone contributed more than limonene to the inhibitory activity of essential oil (Fig 1C and 1D). The contribution of carvone and limonene was normalized by inhibition percent. The average contributions of carvone and limonene were 77.35% and 19.16%, respectively. The contribution of the mixture was 82.47%. The vapor phase effects of dill seed essential oil, the mixture of carvone and limonene, carvone, and limonene were greater than their contact inhibitory effects on mycelial growth. Results of the vapor phase effects are shown in Fig 2. In the vapor phase test at 0.125 μL/mL essential oil, 0.093 μL/mL the mixture, and 0.052 μL/mL carvone in air, fungal development was completely inhibited after 4 d of incubation. Furthermore, 0.041 μL/mL limonene can delay the growth for 2 d. The inhibitory percent of the mixture is also similar to that of essential oil.

Bottom Line: This study aimed to evaluate the inhibitory effects of dill (Anethum graveolens L.) seed essential oil against Sclerotinia sclerotiorum and its mechanism of action.In vivo experiments, the essential oil remarkably suppressed S. sclerotiorum, and considerable morphological alterations were observed in the hyphae and sclerotia.The seed essential oil of A. graveolens can be extensively used in agriculture for preventing the oilseed crops fungal disease.

View Article: PubMed Central - PubMed

Affiliation: Institute of TCM and Natural Products, School of Pharmaceutical Sciences, Wuhan University, Wuhan, P.R. China.

ABSTRACT
This study aimed to evaluate the inhibitory effects of dill (Anethum graveolens L.) seed essential oil against Sclerotinia sclerotiorum and its mechanism of action. The antifungal activities of the two main constituents, namely carvone and limonene, were also measured. Mycelial growth and sclerotial germination were thoroughly inhibited by dill seed essential oil at the 1.00 μL/mL under contact condition and 0.125μL/mL air under vapor condition. Carvone also contributed more than limonene in inhibiting the growth of S. sclerotiorum. Carvone and limonene synergistically inhibited the growth of the fungus. In vivo experiments, the essential oil remarkably suppressed S. sclerotiorum, and considerable morphological alterations were observed in the hyphae and sclerotia. Inhibition of ergosterol synthesis, malate dehydrogenase, succinate dehydrogenase activities, and external medium acidification were investigated to elucidate the antifungal mechanism of the essential oil. The seed essential oil of A. graveolens can be extensively used in agriculture for preventing the oilseed crops fungal disease.

No MeSH data available.


Related in: MedlinePlus