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HOTAIR Interacting with MAPK1 Regulates Ovarian Cancer skov3 Cell Proliferation, Migration, and Invasion.

Yiwei T, Hua H, Hui G, Mao M, Xiang L - Med. Sci. Monit. (2015)

Bottom Line: We detected the mRNA and protein level of MAPK1 when silencing HOTAIR.We detected the expression of HOTAIR when silencing MAPK1.The mRNA and protein level of MAPK1 was decreased when silencing HOTAIR and the mRNA level of HOTAIR was decreased when silencing MAPK1 (p<0.05).

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Early Developmental and Injuries, West China Institutes for Woman and Children's Health, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China (mainland).

ABSTRACT

Background: The aim of this study was to evaluate the effect of when silencing HOTAIR in ovarian cancer skov3 cells on proliferation, migration, and invasion, and to elucidate the mechanism by which this occurs.

Material and methods: We detected the mRNA level of HOTAIR (HOX antisense intergenic RNA) and MAPK1 (mitogen-activated protein kinase 1) in ovarian cancer SKOV3, ES-2, OVCAR3, A2780, and COC1 cell lines. We detected the mRNA level of HOTAIR and MAPK1 in ovarian SKOV3 when transected with miR-1, miR-214-3p, or miR-330-5p. We detected the mRNA and protein level of MAPK1 when silencing HOTAIR. We detected the expression of HOTAIR when silencing MAPK1. Then we detected the proliferation, migration, and invasion in ovarian cancer skov3 after silencing HOTAIR or MAPK1.

Results: The expression of HOTAIR and MAPK1 in ovarian SKOV3, ES-2, and OVCAR3 increased compared with A2780 and COC1 cells (P<0.05). The mRNA level of HOTAIR and MAPK1 in ovarian SKOV3 decreased when transected with miR-1, miR-214-3p, or miR-330-5p compared to negative control (p<0.05). The mRNA and protein level of MAPK1 was decreased when silencing HOTAIR and the mRNA level of HOTAIR was decreased when silencing MAPK1 (p<0.05). The proliferation, migration, and invasion was inhibited in ovarian SKOV3 after silencing HOTAIR or MAPK1 (p<0.05).

Conclusions: HOTAIR can promote proliferation, migration, and invasion in ovarian SKOV3 cells as a competing endogenous RNA.

No MeSH data available.


Related in: MedlinePlus

The expression of MAPK1 in ovarian cancer tissues. (A). Ovarian serous adenocarcinoma; (B). Endometrioid carcinoma; (C) Clear cell carcinoma; (D). Ovarian adenocarcinoma with metastasis; (E). Mucinous adenocarcinoma; (F). Normal ovarian tissues. ×200 magnification.
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f1-medscimonit-21-1856: The expression of MAPK1 in ovarian cancer tissues. (A). Ovarian serous adenocarcinoma; (B). Endometrioid carcinoma; (C) Clear cell carcinoma; (D). Ovarian adenocarcinoma with metastasis; (E). Mucinous adenocarcinoma; (F). Normal ovarian tissues. ×200 magnification.

Mentions: The expression of MAPK1 was significantly higher in ovarian carcinoma samples than normal ovarian samples (Figure 1 and Figure 2F; P<0.05). MAPK1 was predominantly localized on the plasma membrane, and cytoplasm (Figure 1A–1C).


HOTAIR Interacting with MAPK1 Regulates Ovarian Cancer skov3 Cell Proliferation, Migration, and Invasion.

Yiwei T, Hua H, Hui G, Mao M, Xiang L - Med. Sci. Monit. (2015)

The expression of MAPK1 in ovarian cancer tissues. (A). Ovarian serous adenocarcinoma; (B). Endometrioid carcinoma; (C) Clear cell carcinoma; (D). Ovarian adenocarcinoma with metastasis; (E). Mucinous adenocarcinoma; (F). Normal ovarian tissues. ×200 magnification.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4489685&req=5

f1-medscimonit-21-1856: The expression of MAPK1 in ovarian cancer tissues. (A). Ovarian serous adenocarcinoma; (B). Endometrioid carcinoma; (C) Clear cell carcinoma; (D). Ovarian adenocarcinoma with metastasis; (E). Mucinous adenocarcinoma; (F). Normal ovarian tissues. ×200 magnification.
Mentions: The expression of MAPK1 was significantly higher in ovarian carcinoma samples than normal ovarian samples (Figure 1 and Figure 2F; P<0.05). MAPK1 was predominantly localized on the plasma membrane, and cytoplasm (Figure 1A–1C).

Bottom Line: We detected the mRNA and protein level of MAPK1 when silencing HOTAIR.We detected the expression of HOTAIR when silencing MAPK1.The mRNA and protein level of MAPK1 was decreased when silencing HOTAIR and the mRNA level of HOTAIR was decreased when silencing MAPK1 (p<0.05).

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Early Developmental and Injuries, West China Institutes for Woman and Children's Health, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China (mainland).

ABSTRACT

Background: The aim of this study was to evaluate the effect of when silencing HOTAIR in ovarian cancer skov3 cells on proliferation, migration, and invasion, and to elucidate the mechanism by which this occurs.

Material and methods: We detected the mRNA level of HOTAIR (HOX antisense intergenic RNA) and MAPK1 (mitogen-activated protein kinase 1) in ovarian cancer SKOV3, ES-2, OVCAR3, A2780, and COC1 cell lines. We detected the mRNA level of HOTAIR and MAPK1 in ovarian SKOV3 when transected with miR-1, miR-214-3p, or miR-330-5p. We detected the mRNA and protein level of MAPK1 when silencing HOTAIR. We detected the expression of HOTAIR when silencing MAPK1. Then we detected the proliferation, migration, and invasion in ovarian cancer skov3 after silencing HOTAIR or MAPK1.

Results: The expression of HOTAIR and MAPK1 in ovarian SKOV3, ES-2, and OVCAR3 increased compared with A2780 and COC1 cells (P<0.05). The mRNA level of HOTAIR and MAPK1 in ovarian SKOV3 decreased when transected with miR-1, miR-214-3p, or miR-330-5p compared to negative control (p<0.05). The mRNA and protein level of MAPK1 was decreased when silencing HOTAIR and the mRNA level of HOTAIR was decreased when silencing MAPK1 (p<0.05). The proliferation, migration, and invasion was inhibited in ovarian SKOV3 after silencing HOTAIR or MAPK1 (p<0.05).

Conclusions: HOTAIR can promote proliferation, migration, and invasion in ovarian SKOV3 cells as a competing endogenous RNA.

No MeSH data available.


Related in: MedlinePlus