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Mouse positioning device for blood brain barrier FUS exposure

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This causes a rapid accumulation of glucocerebroside in affected persons, which manifests in organ malfunctions and disfiguration, swelling, and severe neurologic complications, among other symptoms... Enzyme replacement therapy can manage the symptoms but when the brain is affected, the treatment is not effective since the enzymes cannot cross the blood brain barrier (BBB)... Imaging of enzyme replacement therapy has shown that only insignificant amounts reach the brain... In this work, we intend to evaluate brain uptake of enzyme replacement after BBB opening mediated by focused ultrasound (FUS) to validate this delivery technique for the treatment of neurologic complications caused by Gaucher’s disease... A stereotactic animal setting for mice was designed using SolidWorks to allow for repetitive FUS exposures of the brain, and was formed with ABS plastic using a MakerBot Replicator 3-D printer... The ability of the positioning system to target the FUS transducer at the same location by the use of the device was measured as well on MR images... The device was able to hold the test subject in the desired position (Figure 2), and was able to ensure that the water level of the positioning table did not rise above the head of the test subjects thanks to markers in the device... These images were performed using the coil embedded into the device and allowed for precise targeting by localizing both the FUS transducer and the brain in a repetitive and reproducible fashion... The results indicate that the system is able to hold a mouse in a repetitive position, regardless of the operator... The ultimate goal will be to perform FUS exposures at a fixed target location, without using the MRI for guidance... We will be then able to perform a PET scan immediately after FUS exposure and validate the delivery of enzymes through the BBB.

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MR images showing the transducer used during trials
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Figure 4: MR images showing the transducer used during trials


Mouse positioning device for blood brain barrier FUS exposure
MR images showing the transducer used during trials
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4489525&req=5

Figure 4: MR images showing the transducer used during trials

View Article: PubMed Central - HTML

AUTOMATICALLY GENERATED EXCERPT
Please rate it.

This causes a rapid accumulation of glucocerebroside in affected persons, which manifests in organ malfunctions and disfiguration, swelling, and severe neurologic complications, among other symptoms... Enzyme replacement therapy can manage the symptoms but when the brain is affected, the treatment is not effective since the enzymes cannot cross the blood brain barrier (BBB)... Imaging of enzyme replacement therapy has shown that only insignificant amounts reach the brain... In this work, we intend to evaluate brain uptake of enzyme replacement after BBB opening mediated by focused ultrasound (FUS) to validate this delivery technique for the treatment of neurologic complications caused by Gaucher’s disease... A stereotactic animal setting for mice was designed using SolidWorks to allow for repetitive FUS exposures of the brain, and was formed with ABS plastic using a MakerBot Replicator 3-D printer... The ability of the positioning system to target the FUS transducer at the same location by the use of the device was measured as well on MR images... The device was able to hold the test subject in the desired position (Figure 2), and was able to ensure that the water level of the positioning table did not rise above the head of the test subjects thanks to markers in the device... These images were performed using the coil embedded into the device and allowed for precise targeting by localizing both the FUS transducer and the brain in a repetitive and reproducible fashion... The results indicate that the system is able to hold a mouse in a repetitive position, regardless of the operator... The ultimate goal will be to perform FUS exposures at a fixed target location, without using the MRI for guidance... We will be then able to perform a PET scan immediately after FUS exposure and validate the delivery of enzymes through the BBB.

No MeSH data available.