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Retrospective distribution of Trypanosoma cruzi I genotypes in Colombia.

León CM, Hernández C, Montilla M, Ramírez JD - Mem. Inst. Oswaldo Cruz (2015)

Bottom Line: In mammal reservoirs, the distribution corresponded to sylvatic TcI in 96% and TcIDom in 4%.Among insect vectors, sylvatic TcI was observed in 48% and TcIDom in 52%.The epidemiological and clinical implications of these findings are discussed herein.

View Article: PubMed Central - PubMed

Affiliation: Red Chagas Colombia, Instituto Nacional de Salud, Bogotá, Colombia.

ABSTRACT
Trypanosoma cruzi is the aetiological agent of Chagas disease, which affects approximately eight million people in the Americas. This parasite exhibits genetic variability, with at least six discrete typing units broadly distributed in the American continent. T. cruzi I (TcI) shows remarkable genetic diversity; a genotype linked to human infections and a domestic cycle of transmission have recently been identified, hence, this strain was named TcIDom. The aim of this work was to describe the spatiotemporal distribution of TcI subpopulations across humans, insect vectors and mammalian reservoirs in Colombia by means of molecular typing targeting the spliced leader intergenic region of mini-exon gene. We analysed 101 TcI isolates and observed a distribution of sylvatic TcI in 70% and TcIDom in 30%. In humans, the ratio was sylvatic TcI in 60% and TcIDom in 40%. In mammal reservoirs, the distribution corresponded to sylvatic TcI in 96% and TcIDom in 4%. Among insect vectors, sylvatic TcI was observed in 48% and TcIDom in 52%. In conclusion, the circulation of TcIDom is emerging in Colombia and this genotype is still adapting to the domestic cycle of transmission. The epidemiological and clinical implications of these findings are discussed herein.

No MeSH data available.


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geographical distribution of TcIDom genotype and sylvatic TcI isolatesdetected in the 101 isolates analysed.
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f02: geographical distribution of TcIDom genotype and sylvatic TcI isolatesdetected in the 101 isolates analysed.

Mentions: We conducted retrospective discrimination for 45 isolates from humans, 32 isolates fromreservoirs and 24 isolates from triatomines originating in 19 departments of Colombiafrom 1984-2012. The DNA bands obtained from the amplification products were 231 and 450bp for TcIDom isolates and sylvatic TcI, respectively (Fig. 1). In a blinded manner, we compared the congruence ofSL-IR and mitochondrial MLST (mtMLST) for detecting TcIDom and were able todetect complete congruence between domestic TcI (SL-IR) and mitochondrial alleles bymeans of MLST. A higher frequency of TcI sylvatic isolates (70%) was observed, followedby TcIDom (30%) across the 101 isolates. Regarding the geographicaldistribution, the departments with a greater number of sylvatic TcI isolates were Nortede Santander and Cundinamarca; for the case of TcIDom, Boyacá showed thehighest prevalence for this genotype (Fig. 2,Table).


Retrospective distribution of Trypanosoma cruzi I genotypes in Colombia.

León CM, Hernández C, Montilla M, Ramírez JD - Mem. Inst. Oswaldo Cruz (2015)

geographical distribution of TcIDom genotype and sylvatic TcI isolatesdetected in the 101 isolates analysed.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4489476&req=5

f02: geographical distribution of TcIDom genotype and sylvatic TcI isolatesdetected in the 101 isolates analysed.
Mentions: We conducted retrospective discrimination for 45 isolates from humans, 32 isolates fromreservoirs and 24 isolates from triatomines originating in 19 departments of Colombiafrom 1984-2012. The DNA bands obtained from the amplification products were 231 and 450bp for TcIDom isolates and sylvatic TcI, respectively (Fig. 1). In a blinded manner, we compared the congruence ofSL-IR and mitochondrial MLST (mtMLST) for detecting TcIDom and were able todetect complete congruence between domestic TcI (SL-IR) and mitochondrial alleles bymeans of MLST. A higher frequency of TcI sylvatic isolates (70%) was observed, followedby TcIDom (30%) across the 101 isolates. Regarding the geographicaldistribution, the departments with a greater number of sylvatic TcI isolates were Nortede Santander and Cundinamarca; for the case of TcIDom, Boyacá showed thehighest prevalence for this genotype (Fig. 2,Table).

Bottom Line: In mammal reservoirs, the distribution corresponded to sylvatic TcI in 96% and TcIDom in 4%.Among insect vectors, sylvatic TcI was observed in 48% and TcIDom in 52%.The epidemiological and clinical implications of these findings are discussed herein.

View Article: PubMed Central - PubMed

Affiliation: Red Chagas Colombia, Instituto Nacional de Salud, Bogotá, Colombia.

ABSTRACT
Trypanosoma cruzi is the aetiological agent of Chagas disease, which affects approximately eight million people in the Americas. This parasite exhibits genetic variability, with at least six discrete typing units broadly distributed in the American continent. T. cruzi I (TcI) shows remarkable genetic diversity; a genotype linked to human infections and a domestic cycle of transmission have recently been identified, hence, this strain was named TcIDom. The aim of this work was to describe the spatiotemporal distribution of TcI subpopulations across humans, insect vectors and mammalian reservoirs in Colombia by means of molecular typing targeting the spliced leader intergenic region of mini-exon gene. We analysed 101 TcI isolates and observed a distribution of sylvatic TcI in 70% and TcIDom in 30%. In humans, the ratio was sylvatic TcI in 60% and TcIDom in 40%. In mammal reservoirs, the distribution corresponded to sylvatic TcI in 96% and TcIDom in 4%. Among insect vectors, sylvatic TcI was observed in 48% and TcIDom in 52%. In conclusion, the circulation of TcIDom is emerging in Colombia and this genotype is still adapting to the domestic cycle of transmission. The epidemiological and clinical implications of these findings are discussed herein.

No MeSH data available.


Related in: MedlinePlus