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Evidences on the Ability of Mycorrhizal Genus Piloderma to Use Organic Nitrogen and Deliver It to Scots Pine.

Heinonsalo J, Sun H, Santalahti M, B├Ącklund K, Hari P, Pumpanen J - PLoS ONE (2015)

Bottom Line: We also tested the effect of Piloderma olivaceum on host plant organic N nutrition in the laboratory.The results showed that Piloderma sp. was highly abundant in the field and produced extracellular proteases, which correlated positively with the gross primary production, temperature and soil respiration.As Piloderma sp. abundance appeared to be seasonally highly variable, recycling of fungal-bound N after hyphal death may therefore be of primary importance for the N cycling in boreal ecosystems.

View Article: PubMed Central - PubMed

Affiliation: Department of Food and Environmental Sciences, University of Helsinki, Helsinki, Finland.

ABSTRACT
Ectomycorrhizal (ECM) symbiosis has been proposed to link plant photosynthesis and soil organic matter (SOM) decomposition through the production of fungal enzymes which promote SOM degradation and nitrogen (N) uptake. However, laboratory and field evidence for the existence of these processes are rare. Piloderma sp., a common ECM genus in boreal forest soil, was chosen as model mycorrhiza for this study. The abundance of Piloderma sp. was studied in root tips and soil over one growing season and in winter. Protease production was measured from ectomycorrhiza and soil solution in the field and pure fungal cultures. We also tested the effect of Piloderma olivaceum on host plant organic N nutrition in the laboratory. The results showed that Piloderma sp. was highly abundant in the field and produced extracellular proteases, which correlated positively with the gross primary production, temperature and soil respiration. In the laboratory, Piloderma olivaceum could improve the ability of Pinus sylvestris L. to utilize N from extragenous proteins. We suggest that ECM fungi, although potentially retaining N in their hyphae, are important in forest C and N cycling due to their ability to access proteinaeous N. As Piloderma sp. abundance appeared to be seasonally highly variable, recycling of fungal-bound N after hyphal death may therefore be of primary importance for the N cycling in boreal ecosystems.

No MeSH data available.


Protease production of ectomycorrhizal culture collection isolates and field isolates from the studied Scots pine roots.For details of the fungal cultures used in this study, see Table 1. Independent-samples non-parametric Kruskal-Wallis test with pairwise comparisons were performed (N = 3). The different letters above standard deviation bars indicate statistical difference (P<0.05), adjusted for multiple comparisons.
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pone.0131561.g002: Protease production of ectomycorrhizal culture collection isolates and field isolates from the studied Scots pine roots.For details of the fungal cultures used in this study, see Table 1. Independent-samples non-parametric Kruskal-Wallis test with pairwise comparisons were performed (N = 3). The different letters above standard deviation bars indicate statistical difference (P<0.05), adjusted for multiple comparisons.

Mentions: P. olivaceum produced high quantities of protease as measured using the PF0100 Protease Fluorescent Detection Kit (Fig 2). Suillus variegatus produced the second highest quantities of proteases, followed by other Suillus sp. One of the two S. variegatus strains produced statistically as much proteases as P. olivaceum. However, Cenococcum geophilum did not show any ability to produce proteases (Fig 2).


Evidences on the Ability of Mycorrhizal Genus Piloderma to Use Organic Nitrogen and Deliver It to Scots Pine.

Heinonsalo J, Sun H, Santalahti M, B├Ącklund K, Hari P, Pumpanen J - PLoS ONE (2015)

Protease production of ectomycorrhizal culture collection isolates and field isolates from the studied Scots pine roots.For details of the fungal cultures used in this study, see Table 1. Independent-samples non-parametric Kruskal-Wallis test with pairwise comparisons were performed (N = 3). The different letters above standard deviation bars indicate statistical difference (P<0.05), adjusted for multiple comparisons.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4489387&req=5

pone.0131561.g002: Protease production of ectomycorrhizal culture collection isolates and field isolates from the studied Scots pine roots.For details of the fungal cultures used in this study, see Table 1. Independent-samples non-parametric Kruskal-Wallis test with pairwise comparisons were performed (N = 3). The different letters above standard deviation bars indicate statistical difference (P<0.05), adjusted for multiple comparisons.
Mentions: P. olivaceum produced high quantities of protease as measured using the PF0100 Protease Fluorescent Detection Kit (Fig 2). Suillus variegatus produced the second highest quantities of proteases, followed by other Suillus sp. One of the two S. variegatus strains produced statistically as much proteases as P. olivaceum. However, Cenococcum geophilum did not show any ability to produce proteases (Fig 2).

Bottom Line: We also tested the effect of Piloderma olivaceum on host plant organic N nutrition in the laboratory.The results showed that Piloderma sp. was highly abundant in the field and produced extracellular proteases, which correlated positively with the gross primary production, temperature and soil respiration.As Piloderma sp. abundance appeared to be seasonally highly variable, recycling of fungal-bound N after hyphal death may therefore be of primary importance for the N cycling in boreal ecosystems.

View Article: PubMed Central - PubMed

Affiliation: Department of Food and Environmental Sciences, University of Helsinki, Helsinki, Finland.

ABSTRACT
Ectomycorrhizal (ECM) symbiosis has been proposed to link plant photosynthesis and soil organic matter (SOM) decomposition through the production of fungal enzymes which promote SOM degradation and nitrogen (N) uptake. However, laboratory and field evidence for the existence of these processes are rare. Piloderma sp., a common ECM genus in boreal forest soil, was chosen as model mycorrhiza for this study. The abundance of Piloderma sp. was studied in root tips and soil over one growing season and in winter. Protease production was measured from ectomycorrhiza and soil solution in the field and pure fungal cultures. We also tested the effect of Piloderma olivaceum on host plant organic N nutrition in the laboratory. The results showed that Piloderma sp. was highly abundant in the field and produced extracellular proteases, which correlated positively with the gross primary production, temperature and soil respiration. In the laboratory, Piloderma olivaceum could improve the ability of Pinus sylvestris L. to utilize N from extragenous proteins. We suggest that ECM fungi, although potentially retaining N in their hyphae, are important in forest C and N cycling due to their ability to access proteinaeous N. As Piloderma sp. abundance appeared to be seasonally highly variable, recycling of fungal-bound N after hyphal death may therefore be of primary importance for the N cycling in boreal ecosystems.

No MeSH data available.