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Ornithodoros faccinii n. sp. (Acari: Ixodida: Argasidae) parasitizing the frog Thoropa miliaris (Amphibia: Anura: Cycloramphidae) in Brazil.

Barros-Battesti DM, Landulfo GA, Luz HR, Marcili A, Onofrio VC, Famadas KM - Parasit Vectors (2015)

Bottom Line: However, the larval morphology of the new species is unique.The mitochondrial 16S rDNA partial sequence of O. faccinii generated in the present study was deposited in GenBank under the number KP861242.This is the first report of argasid ticks on frogs in Brazil, the second on frogs and the third on Amphibia in the Neotropical region.

View Article: PubMed Central - PubMed

Affiliation: Special Laboratory of Zoological Collection, Butantan Institute, São Paulo, SP, Brazil. darci.battesti@butantan.gov.br.

ABSTRACT

Background: Most argasid ticks from the Neotropical region are parasites of mammals and birds, with a few records from reptiles. Many species of the genus Ornithodoros are known only through larval descriptions, and their chaetotaxy and morphological characteristics have been used to separate the taxa. In the present study, we describe the larva and the nymph of first instar of a new species of the genus Ornithodoros that was collected from frogs of the species Thoropa miliaris.

Methods: Larvae of Ornithodoros were collected from frogs of the species T. miliaris at waterfalls in the state of Rio de Janeiro, southeastern Brazil. The larval and nymphal description was based on optical and scanning electron microscopy. Molecular analysis using the argasid 16S rRNA sequences available in GenBank was also conducted.

Results: Ornithodoros faccinii sp. n. is closely related to Ornithodoros clarki Jones & Clifford, Ornithodoros marinkellei Kohls, Clifford & Jones, Ornithodoros capensis Neumann and Ornithodoros sawaii Kitaoka & Susuki. However, the larval morphology of the new species is unique. The mitochondrial 16S rDNA partial sequence of O. faccinii generated in the present study was deposited in GenBank under the number KP861242.

Conclusions: The larvae collected from Thoropa miliaris are a new species, Ornithodoros faccinii n. sp. This is the first report of argasid ticks on frogs in Brazil, the second on frogs and the third on Amphibia in the Neotropical region.

No MeSH data available.


Phylogenetic tree based on the 16S rDNA ticks. The alignment was produced using Clustal X and the tree was inferred by means of the MP method with 500 replicates of random addition taxa. The species Ixodes holocyclus and Ixodes uriae were used as outgroup. The Bayesian support (posterior probability) values are derived from 1,000,000 replicates.
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Fig5: Phylogenetic tree based on the 16S rDNA ticks. The alignment was produced using Clustal X and the tree was inferred by means of the MP method with 500 replicates of random addition taxa. The species Ixodes holocyclus and Ixodes uriae were used as outgroup. The Bayesian support (posterior probability) values are derived from 1,000,000 replicates.

Mentions: The phylogenetic relationships based on a partial sequence of the mitochondrial 16S rDNA gene (Figure 5) grouped O. faccinii n. sp. with O. capensis and Ornithodoros sawaii Kitaoka & Susuki, 1973, within a strongly supported branch (85% bootstrap and 0.78 posterior probability). The sequence divergence between O. faccinii n. sp. and O. capensis was 19.8% and between O. faccinii n. sp. and O. sawaii, 22%. The larva of the new species is also related to larvae of O. marinkellei and O. fonsecai.Figure 5


Ornithodoros faccinii n. sp. (Acari: Ixodida: Argasidae) parasitizing the frog Thoropa miliaris (Amphibia: Anura: Cycloramphidae) in Brazil.

Barros-Battesti DM, Landulfo GA, Luz HR, Marcili A, Onofrio VC, Famadas KM - Parasit Vectors (2015)

Phylogenetic tree based on the 16S rDNA ticks. The alignment was produced using Clustal X and the tree was inferred by means of the MP method with 500 replicates of random addition taxa. The species Ixodes holocyclus and Ixodes uriae were used as outgroup. The Bayesian support (posterior probability) values are derived from 1,000,000 replicates.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4489115&req=5

Fig5: Phylogenetic tree based on the 16S rDNA ticks. The alignment was produced using Clustal X and the tree was inferred by means of the MP method with 500 replicates of random addition taxa. The species Ixodes holocyclus and Ixodes uriae were used as outgroup. The Bayesian support (posterior probability) values are derived from 1,000,000 replicates.
Mentions: The phylogenetic relationships based on a partial sequence of the mitochondrial 16S rDNA gene (Figure 5) grouped O. faccinii n. sp. with O. capensis and Ornithodoros sawaii Kitaoka & Susuki, 1973, within a strongly supported branch (85% bootstrap and 0.78 posterior probability). The sequence divergence between O. faccinii n. sp. and O. capensis was 19.8% and between O. faccinii n. sp. and O. sawaii, 22%. The larva of the new species is also related to larvae of O. marinkellei and O. fonsecai.Figure 5

Bottom Line: However, the larval morphology of the new species is unique.The mitochondrial 16S rDNA partial sequence of O. faccinii generated in the present study was deposited in GenBank under the number KP861242.This is the first report of argasid ticks on frogs in Brazil, the second on frogs and the third on Amphibia in the Neotropical region.

View Article: PubMed Central - PubMed

Affiliation: Special Laboratory of Zoological Collection, Butantan Institute, São Paulo, SP, Brazil. darci.battesti@butantan.gov.br.

ABSTRACT

Background: Most argasid ticks from the Neotropical region are parasites of mammals and birds, with a few records from reptiles. Many species of the genus Ornithodoros are known only through larval descriptions, and their chaetotaxy and morphological characteristics have been used to separate the taxa. In the present study, we describe the larva and the nymph of first instar of a new species of the genus Ornithodoros that was collected from frogs of the species Thoropa miliaris.

Methods: Larvae of Ornithodoros were collected from frogs of the species T. miliaris at waterfalls in the state of Rio de Janeiro, southeastern Brazil. The larval and nymphal description was based on optical and scanning electron microscopy. Molecular analysis using the argasid 16S rRNA sequences available in GenBank was also conducted.

Results: Ornithodoros faccinii sp. n. is closely related to Ornithodoros clarki Jones & Clifford, Ornithodoros marinkellei Kohls, Clifford & Jones, Ornithodoros capensis Neumann and Ornithodoros sawaii Kitaoka & Susuki. However, the larval morphology of the new species is unique. The mitochondrial 16S rDNA partial sequence of O. faccinii generated in the present study was deposited in GenBank under the number KP861242.

Conclusions: The larvae collected from Thoropa miliaris are a new species, Ornithodoros faccinii n. sp. This is the first report of argasid ticks on frogs in Brazil, the second on frogs and the third on Amphibia in the Neotropical region.

No MeSH data available.