Limits...
Ornithodoros faccinii n. sp. (Acari: Ixodida: Argasidae) parasitizing the frog Thoropa miliaris (Amphibia: Anura: Cycloramphidae) in Brazil.

Barros-Battesti DM, Landulfo GA, Luz HR, Marcili A, Onofrio VC, Famadas KM - Parasit Vectors (2015)

Bottom Line: However, the larval morphology of the new species is unique.The mitochondrial 16S rDNA partial sequence of O. faccinii generated in the present study was deposited in GenBank under the number KP861242.This is the first report of argasid ticks on frogs in Brazil, the second on frogs and the third on Amphibia in the Neotropical region.

View Article: PubMed Central - PubMed

Affiliation: Special Laboratory of Zoological Collection, Butantan Institute, São Paulo, SP, Brazil. darci.battesti@butantan.gov.br.

ABSTRACT

Background: Most argasid ticks from the Neotropical region are parasites of mammals and birds, with a few records from reptiles. Many species of the genus Ornithodoros are known only through larval descriptions, and their chaetotaxy and morphological characteristics have been used to separate the taxa. In the present study, we describe the larva and the nymph of first instar of a new species of the genus Ornithodoros that was collected from frogs of the species Thoropa miliaris.

Methods: Larvae of Ornithodoros were collected from frogs of the species T. miliaris at waterfalls in the state of Rio de Janeiro, southeastern Brazil. The larval and nymphal description was based on optical and scanning electron microscopy. Molecular analysis using the argasid 16S rRNA sequences available in GenBank was also conducted.

Results: Ornithodoros faccinii sp. n. is closely related to Ornithodoros clarki Jones & Clifford, Ornithodoros marinkellei Kohls, Clifford & Jones, Ornithodoros capensis Neumann and Ornithodoros sawaii Kitaoka & Susuki. However, the larval morphology of the new species is unique. The mitochondrial 16S rDNA partial sequence of O. faccinii generated in the present study was deposited in GenBank under the number KP861242.

Conclusions: The larvae collected from Thoropa miliaris are a new species, Ornithodoros faccinii n. sp. This is the first report of argasid ticks on frogs in Brazil, the second on frogs and the third on Amphibia in the Neotropical region.

No MeSH data available.


Scanning electron microscopy of idiosoma and capitulum of larvae of Ornithodoros faccinii n. sp. A. Idiosoma, dorsal view. B. Part of basis capituli and hypostome. C. Idiosoma, ventral view. D. Dorsal plate. E. Detail of ventral idiosoma, showing the pair of setae VPL (ventral posterolateral) (black arrow). Scale bars: A. 500 μm; B. 50 μm; C. 500 μm; D. 100 μm; E. 250 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4489115&req=5

Fig1: Scanning electron microscopy of idiosoma and capitulum of larvae of Ornithodoros faccinii n. sp. A. Idiosoma, dorsal view. B. Part of basis capituli and hypostome. C. Idiosoma, ventral view. D. Dorsal plate. E. Detail of ventral idiosoma, showing the pair of setae VPL (ventral posterolateral) (black arrow). Scale bars: A. 500 μm; B. 50 μm; C. 500 μm; D. 100 μm; E. 250 μm.

Mentions: Larva with elongated dorsal plate, almost rectangular; the anterior and posterior margins rounded with sides parallel. Idiosoma dorsal with 10 pairs of dorsolateral setae and 3 pairs of central dorsal setae. Idiosoma ventral with 7 pairs of setae (3 sternal, 3 circumanal and 1 ventrolateral pairs) plus 1 anal pair. Hypostome long, pointed, dental formula 3/3 in the anterior third, then 2/2 posteriorly to the base. Presence of small spurs at the base of hypostome in the lateral position. Trochanter of palpi with spurs in the internal side and ventrally, some of them are bifid. Nymphs of first instar with reduced camerostome. Hood and cheeks absent. First pair of posthypostomal setae (Ph1) arising at level of insertion of article II of palpi and half of hypostome. Second pair of posthypostomal setae (Ph2) very short, not reaching the hypostomal basis and corresponding to 1/4 of length of Ph1. Hypostome notched in the apex, dental formula 4/4 apically (3 rows) then 3/3 (3 rows) and 2/2 (3 rows) until posterior margin of article II. Body with mammillae covering both ventral and dorsal surfaces. Genital primordium present. Capsule of Haller’s organ U-shaped.


Ornithodoros faccinii n. sp. (Acari: Ixodida: Argasidae) parasitizing the frog Thoropa miliaris (Amphibia: Anura: Cycloramphidae) in Brazil.

Barros-Battesti DM, Landulfo GA, Luz HR, Marcili A, Onofrio VC, Famadas KM - Parasit Vectors (2015)

Scanning electron microscopy of idiosoma and capitulum of larvae of Ornithodoros faccinii n. sp. A. Idiosoma, dorsal view. B. Part of basis capituli and hypostome. C. Idiosoma, ventral view. D. Dorsal plate. E. Detail of ventral idiosoma, showing the pair of setae VPL (ventral posterolateral) (black arrow). Scale bars: A. 500 μm; B. 50 μm; C. 500 μm; D. 100 μm; E. 250 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4489115&req=5

Fig1: Scanning electron microscopy of idiosoma and capitulum of larvae of Ornithodoros faccinii n. sp. A. Idiosoma, dorsal view. B. Part of basis capituli and hypostome. C. Idiosoma, ventral view. D. Dorsal plate. E. Detail of ventral idiosoma, showing the pair of setae VPL (ventral posterolateral) (black arrow). Scale bars: A. 500 μm; B. 50 μm; C. 500 μm; D. 100 μm; E. 250 μm.
Mentions: Larva with elongated dorsal plate, almost rectangular; the anterior and posterior margins rounded with sides parallel. Idiosoma dorsal with 10 pairs of dorsolateral setae and 3 pairs of central dorsal setae. Idiosoma ventral with 7 pairs of setae (3 sternal, 3 circumanal and 1 ventrolateral pairs) plus 1 anal pair. Hypostome long, pointed, dental formula 3/3 in the anterior third, then 2/2 posteriorly to the base. Presence of small spurs at the base of hypostome in the lateral position. Trochanter of palpi with spurs in the internal side and ventrally, some of them are bifid. Nymphs of first instar with reduced camerostome. Hood and cheeks absent. First pair of posthypostomal setae (Ph1) arising at level of insertion of article II of palpi and half of hypostome. Second pair of posthypostomal setae (Ph2) very short, not reaching the hypostomal basis and corresponding to 1/4 of length of Ph1. Hypostome notched in the apex, dental formula 4/4 apically (3 rows) then 3/3 (3 rows) and 2/2 (3 rows) until posterior margin of article II. Body with mammillae covering both ventral and dorsal surfaces. Genital primordium present. Capsule of Haller’s organ U-shaped.

Bottom Line: However, the larval morphology of the new species is unique.The mitochondrial 16S rDNA partial sequence of O. faccinii generated in the present study was deposited in GenBank under the number KP861242.This is the first report of argasid ticks on frogs in Brazil, the second on frogs and the third on Amphibia in the Neotropical region.

View Article: PubMed Central - PubMed

Affiliation: Special Laboratory of Zoological Collection, Butantan Institute, São Paulo, SP, Brazil. darci.battesti@butantan.gov.br.

ABSTRACT

Background: Most argasid ticks from the Neotropical region are parasites of mammals and birds, with a few records from reptiles. Many species of the genus Ornithodoros are known only through larval descriptions, and their chaetotaxy and morphological characteristics have been used to separate the taxa. In the present study, we describe the larva and the nymph of first instar of a new species of the genus Ornithodoros that was collected from frogs of the species Thoropa miliaris.

Methods: Larvae of Ornithodoros were collected from frogs of the species T. miliaris at waterfalls in the state of Rio de Janeiro, southeastern Brazil. The larval and nymphal description was based on optical and scanning electron microscopy. Molecular analysis using the argasid 16S rRNA sequences available in GenBank was also conducted.

Results: Ornithodoros faccinii sp. n. is closely related to Ornithodoros clarki Jones & Clifford, Ornithodoros marinkellei Kohls, Clifford & Jones, Ornithodoros capensis Neumann and Ornithodoros sawaii Kitaoka & Susuki. However, the larval morphology of the new species is unique. The mitochondrial 16S rDNA partial sequence of O. faccinii generated in the present study was deposited in GenBank under the number KP861242.

Conclusions: The larvae collected from Thoropa miliaris are a new species, Ornithodoros faccinii n. sp. This is the first report of argasid ticks on frogs in Brazil, the second on frogs and the third on Amphibia in the Neotropical region.

No MeSH data available.