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Glucocorticoid receptor is involved in the breed-dependent transcriptional regulation of 3β-hydroxysteroid dehydrogenase in the liver of preweaning piglets.

Li X, Jia Y, Li R, Sun Z, Li X, Sui S, Zhao R - BMC Vet. Res. (2015)

Bottom Line: The hepatic expression of 3β-HSD mRNA was significantly lower (P < 0.01) in EHL piglets compared to that in LW piglets.The nucleoprotein contents of AR (P < 0.05), GR (P < 0.01) and phospho-Ser(211)GR (P < 0.01) were significantly higher in the liver of EHL piglets.These results indicate that GR binding to 3β-HSD promoter is involved in the breed-dependent 3β-HSD expression in the liver of piglets.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, Nanjing, 210095, P. R. China. lixianlovelv@163.com.

ABSTRACT

Background: Hepatic 3β-hydroxysteroid dehydrogenase (3β-HSD) plays an important role in steroid inactivation and catabolism. Serum concentrations of steroid hormones differ significantly between breeds in pigs, however the molecular mechanism regulating hepatic 3β-HSD expression in different breeds of pigs is poorly understood. In the present study, we used preweaning purebred male Large White (LW) and Erhualian (EHL) piglets as model to investigate the breed difference in the expression and regulation of 3β-HSD gene in porcine liver.

Results: The hepatic expression of 3β-HSD mRNA was significantly lower (P < 0.01) in EHL piglets compared to that in LW piglets. Significant breed differences were detected for the hepatic expression of transcription factors such as androgen receptor (AR), glucocorticoid receptor (GR), and CCAAT/enhancer binding protein β (C/EBPβ). The nucleoprotein contents of AR (P < 0.05), GR (P < 0.01) and phospho-Ser(211)GR (P < 0.01) were significantly higher in the liver of EHL piglets. Chromatin immunoprecipitation (ChIP) assay demonstrated significantly lower binding of GR, but not AR or C/EBPβ, to 3β-HSD gene promoter in EHL piglets (P < 0.05). GR was not detected to interact with C/EBPβ or AR in the co-immunoprecipitation analysis.

Conclusions: These results indicate that GR binding to 3β-HSD promoter is involved in the breed-dependent 3β-HSD expression in the liver of piglets.

No MeSH data available.


Relative Hepatic expression of transcription factors. (a) Hepatic expression of genes involved in 3β-HSD transcription. (b) Nucleoprotein content of AR, GR, phospho-Ser211GR and C/EBPβ in the liver. Values are means ± SEM, n = 5 for LW or 6 for EHL. * P < 0.05, compared with LW
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Fig2: Relative Hepatic expression of transcription factors. (a) Hepatic expression of genes involved in 3β-HSD transcription. (b) Nucleoprotein content of AR, GR, phospho-Ser211GR and C/EBPβ in the liver. Values are means ± SEM, n = 5 for LW or 6 for EHL. * P < 0.05, compared with LW

Mentions: The mRNA expression of AR tended to be lower in the liver of EHL piglets compared to that in LW (P = 0.07) (Fig. 2a). However, the hepatic nucleoprotein content of AR was significantly higher in EHL piglets (P < 0.05) (Fig. 2b). Similarly, the hepatic mRNA expression and nucleoprotein contents of GR (P < 0.01) and phospho-Ser211GR (P < 0.01) were significantly higher in EHL piglets (Fig. 2a and b). C/EBPβ mRNA expression was significantly lower in the liver of EHL piglets (P < 0.05), but no breed difference was observed for the protein content (Fig. 2a and b).Fig. 2


Glucocorticoid receptor is involved in the breed-dependent transcriptional regulation of 3β-hydroxysteroid dehydrogenase in the liver of preweaning piglets.

Li X, Jia Y, Li R, Sun Z, Li X, Sui S, Zhao R - BMC Vet. Res. (2015)

Relative Hepatic expression of transcription factors. (a) Hepatic expression of genes involved in 3β-HSD transcription. (b) Nucleoprotein content of AR, GR, phospho-Ser211GR and C/EBPβ in the liver. Values are means ± SEM, n = 5 for LW or 6 for EHL. * P < 0.05, compared with LW
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4489036&req=5

Fig2: Relative Hepatic expression of transcription factors. (a) Hepatic expression of genes involved in 3β-HSD transcription. (b) Nucleoprotein content of AR, GR, phospho-Ser211GR and C/EBPβ in the liver. Values are means ± SEM, n = 5 for LW or 6 for EHL. * P < 0.05, compared with LW
Mentions: The mRNA expression of AR tended to be lower in the liver of EHL piglets compared to that in LW (P = 0.07) (Fig. 2a). However, the hepatic nucleoprotein content of AR was significantly higher in EHL piglets (P < 0.05) (Fig. 2b). Similarly, the hepatic mRNA expression and nucleoprotein contents of GR (P < 0.01) and phospho-Ser211GR (P < 0.01) were significantly higher in EHL piglets (Fig. 2a and b). C/EBPβ mRNA expression was significantly lower in the liver of EHL piglets (P < 0.05), but no breed difference was observed for the protein content (Fig. 2a and b).Fig. 2

Bottom Line: The hepatic expression of 3β-HSD mRNA was significantly lower (P < 0.01) in EHL piglets compared to that in LW piglets.The nucleoprotein contents of AR (P < 0.05), GR (P < 0.01) and phospho-Ser(211)GR (P < 0.01) were significantly higher in the liver of EHL piglets.These results indicate that GR binding to 3β-HSD promoter is involved in the breed-dependent 3β-HSD expression in the liver of piglets.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, Nanjing, 210095, P. R. China. lixianlovelv@163.com.

ABSTRACT

Background: Hepatic 3β-hydroxysteroid dehydrogenase (3β-HSD) plays an important role in steroid inactivation and catabolism. Serum concentrations of steroid hormones differ significantly between breeds in pigs, however the molecular mechanism regulating hepatic 3β-HSD expression in different breeds of pigs is poorly understood. In the present study, we used preweaning purebred male Large White (LW) and Erhualian (EHL) piglets as model to investigate the breed difference in the expression and regulation of 3β-HSD gene in porcine liver.

Results: The hepatic expression of 3β-HSD mRNA was significantly lower (P < 0.01) in EHL piglets compared to that in LW piglets. Significant breed differences were detected for the hepatic expression of transcription factors such as androgen receptor (AR), glucocorticoid receptor (GR), and CCAAT/enhancer binding protein β (C/EBPβ). The nucleoprotein contents of AR (P < 0.05), GR (P < 0.01) and phospho-Ser(211)GR (P < 0.01) were significantly higher in the liver of EHL piglets. Chromatin immunoprecipitation (ChIP) assay demonstrated significantly lower binding of GR, but not AR or C/EBPβ, to 3β-HSD gene promoter in EHL piglets (P < 0.05). GR was not detected to interact with C/EBPβ or AR in the co-immunoprecipitation analysis.

Conclusions: These results indicate that GR binding to 3β-HSD promoter is involved in the breed-dependent 3β-HSD expression in the liver of piglets.

No MeSH data available.