Limits...
Cytogenetic analysis of Scinaxauratus and Scinaxeurydice (Anura, Hylidae) with emphasis on cytotaxonomy.

Nogueira L, Paim F, Diniz D, Solé M, Affonso P, Siqueira S, Sampaio I - Comp Cytogenet (2015)

Bottom Line: Both species shared the same karyotype, location of active nucleolar organizer regions on pair 11 and GC-rich heterochromatin, as reported for most species in Scinaxruber clade.Interpopulation chromosomal variation was observed in Scinaxeurydice, indicating the occurrence of cryptic species.The mapping of 18S ribosomal genes by FISH is reported for the first time in both species.

View Article: PubMed Central - HTML - PubMed

Affiliation: Instituto de Estudos Costeiros, Universidade Federal do Pará, Alameda Leandro Ribeiro s/n, Aldeia, Bragança, PA, CEP 68600-000.

ABSTRACT
Scinax Wagler, 1830 is a species-rich genus of amphibians with relatively few detailed chromosomal reports. In this work, cytogenetic analyses of Scinaxauratus (Wied-Neuwied, 1821) and Scinaxeurydice (Bokermann, 1968) were carried out based on conventional (Giemsa staining, Ag-NOR and C-banding) and cytomolecular (base-specific fluorochrome staining and fluorescence in situ hybridization - FISH of ribosomal probes) techniques. Both species shared the same karyotype, location of active nucleolar organizer regions on pair 11 and GC-rich heterochromatin, as reported for most species in Scinaxruber clade. Interpopulation chromosomal variation was observed in Scinaxeurydice, indicating the occurrence of cryptic species. The mapping of 18S ribosomal genes by FISH is reported for the first time in both species.

No MeSH data available.


Karyotypes of Scinaxauratus (a, c, e) and Scinaxeurydice (b, d, f) after Giemsa-staining (a, b), C-banding (c, d) and base-specific fluorochrome staining (e, f). The NOR-bearing chromosomes after silver nitrate staining and FISH with 18S rDNA probes of each species are shown in boxes. Bar = 10 µm.
© Copyright Policy - creative-commons-attribution
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4488969&req=5

Figure 1: Karyotypes of Scinaxauratus (a, c, e) and Scinaxeurydice (b, d, f) after Giemsa-staining (a, b), C-banding (c, d) and base-specific fluorochrome staining (e, f). The NOR-bearing chromosomes after silver nitrate staining and FISH with 18S rDNA probes of each species are shown in boxes. Bar = 10 µm.

Mentions: Scinaxauratus and Scinaxeurydice presented 2n = 24 and FN = 48 besides sharing the same chromosomal formula: 16 metacentric (pairs 1, 2, 7, 8, 9, 10, 11 and 12) and eight submetacentric (pairs 3, 4, 5 and 6) chromosomes (Table 2; Fig. 1).


Cytogenetic analysis of Scinaxauratus and Scinaxeurydice (Anura, Hylidae) with emphasis on cytotaxonomy.

Nogueira L, Paim F, Diniz D, Solé M, Affonso P, Siqueira S, Sampaio I - Comp Cytogenet (2015)

Karyotypes of Scinaxauratus (a, c, e) and Scinaxeurydice (b, d, f) after Giemsa-staining (a, b), C-banding (c, d) and base-specific fluorochrome staining (e, f). The NOR-bearing chromosomes after silver nitrate staining and FISH with 18S rDNA probes of each species are shown in boxes. Bar = 10 µm.
© Copyright Policy - creative-commons-attribution
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488969&req=5

Figure 1: Karyotypes of Scinaxauratus (a, c, e) and Scinaxeurydice (b, d, f) after Giemsa-staining (a, b), C-banding (c, d) and base-specific fluorochrome staining (e, f). The NOR-bearing chromosomes after silver nitrate staining and FISH with 18S rDNA probes of each species are shown in boxes. Bar = 10 µm.
Mentions: Scinaxauratus and Scinaxeurydice presented 2n = 24 and FN = 48 besides sharing the same chromosomal formula: 16 metacentric (pairs 1, 2, 7, 8, 9, 10, 11 and 12) and eight submetacentric (pairs 3, 4, 5 and 6) chromosomes (Table 2; Fig. 1).

Bottom Line: Both species shared the same karyotype, location of active nucleolar organizer regions on pair 11 and GC-rich heterochromatin, as reported for most species in Scinaxruber clade.Interpopulation chromosomal variation was observed in Scinaxeurydice, indicating the occurrence of cryptic species.The mapping of 18S ribosomal genes by FISH is reported for the first time in both species.

View Article: PubMed Central - HTML - PubMed

Affiliation: Instituto de Estudos Costeiros, Universidade Federal do Pará, Alameda Leandro Ribeiro s/n, Aldeia, Bragança, PA, CEP 68600-000.

ABSTRACT
Scinax Wagler, 1830 is a species-rich genus of amphibians with relatively few detailed chromosomal reports. In this work, cytogenetic analyses of Scinaxauratus (Wied-Neuwied, 1821) and Scinaxeurydice (Bokermann, 1968) were carried out based on conventional (Giemsa staining, Ag-NOR and C-banding) and cytomolecular (base-specific fluorochrome staining and fluorescence in situ hybridization - FISH of ribosomal probes) techniques. Both species shared the same karyotype, location of active nucleolar organizer regions on pair 11 and GC-rich heterochromatin, as reported for most species in Scinaxruber clade. Interpopulation chromosomal variation was observed in Scinaxeurydice, indicating the occurrence of cryptic species. The mapping of 18S ribosomal genes by FISH is reported for the first time in both species.

No MeSH data available.