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Genomic in situ hybridization identifies parental chromosomes in hybrid scallop (Bivalvia, Pectinoida, Pectinidae) between female Chlamysfarreri and male Argopectenirradiansirradians.

Huang X, Bi K, Lu W, Wang S, Zhang L, Bao Z - Comp Cytogenet (2015)

Bottom Line: Interspecific crossing was artificially carried out between Chlamysfarreri (Jones & Preston, 1904) ♀ and Argopectenirradiansirradians (Lamarck, 1819) ♂, two of the dominant cultivated scallop species in China.The number of chromosomes in 66.38% of the metaphases was 2n = 35 and the karyotype was 2n = 3 m + 5 sm + 16 st + 11 t.After GISH, two parental genomes were clearly distinguished in hybrids, most of which comprised 19 chromosomes derived from their female parent (Chlamysfarreri) and 16 chromosomes from their male parent (Argopectenirradiansirradians).

View Article: PubMed Central - HTML - PubMed

Affiliation: Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China.

ABSTRACT
Interspecific crossing was artificially carried out between Chlamysfarreri (Jones & Preston, 1904) ♀ and Argopectenirradiansirradians (Lamarck, 1819) ♂, two of the dominant cultivated scallop species in China. Genomic in situ hybridization (GISH) was used to examine the chromosome constitution and variation in hybrids at early embryonic stage. The number of chromosomes in 66.38% of the metaphases was 2n = 35 and the karyotype was 2n = 3 m + 5 sm + 16 st + 11 t. After GISH, two parental genomes were clearly distinguished in hybrids, most of which comprised 19 chromosomes derived from their female parent (Chlamysfarreri) and 16 chromosomes from their male parent (Argopectenirradiansirradians). Some chromosome elimination and fragmentation was also observed in the hybrids.

No MeSH data available.


Related in: MedlinePlus

Representative metaphase chromosomes and karyotypes of F1 hybrids of Chlamysfarreri ♀ × Argopectenirradiansirradians ♂ examined by GISH. Chromosomes were labeled by FITC (green) and counterstained by PI (red). In (A, B), chromosomes originated from Argopectenirradiansirradians were painted green using the labeled genomic DNA probes from Argopectenirradiansirradians. In (C, D), chromosomes from Chlamysfarreri were painted green using the labeled genomic DNA probes from Chlamysfarreri. m: metacentric, sm: submetacentric, st: subtelocentric, t: telocentric. Bars = 5 µm.
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Figure 1: Representative metaphase chromosomes and karyotypes of F1 hybrids of Chlamysfarreri ♀ × Argopectenirradiansirradians ♂ examined by GISH. Chromosomes were labeled by FITC (green) and counterstained by PI (red). In (A, B), chromosomes originated from Argopectenirradiansirradians were painted green using the labeled genomic DNA probes from Argopectenirradiansirradians. In (C, D), chromosomes from Chlamysfarreri were painted green using the labeled genomic DNA probes from Chlamysfarreri. m: metacentric, sm: submetacentric, st: subtelocentric, t: telocentric. Bars = 5 µm.

Mentions: The chromosome number of hybrids was determined by observing more than one hundred metaphase plates. The statistic results showed that 66.38% of 116 metaphase plates present a diploid component of 2n = 35 in the hybrids. Ten metaphase plates of hybrids were selected to measure arm length and calculate arm ratio and relative length of chromosomes. The karyotype of hybrids is 2n = 35 = 3m + 5sm + 16st + 11t. Typical mitotic spread of the hybrids was shown in Figure 1. The karyotype of Chlamysfarreri is 2n = 38 = 6m + 10sm + 22st (Wang et al. 1990), while that of Argopectenirradiansirradians is 2n = 32 = 10st + 22t (Wang and Guo 2004). Most of the hybrid metaphase plates had a diploid chromosome number of 35, as expected from the parental haploid complements. According to the chromosome configuration, all 3 metacentric chromosomes belonged to Chlamysfarreri, but not Argopectenirradiansirradians. And all 11 telocentric chromosomes, on the contrary, belonged to Argopectenirradiansirradians but not Chlamysfarreri. These chromosome morphological characteristics can be used for chromosome identification in the hybrid metaphases.


Genomic in situ hybridization identifies parental chromosomes in hybrid scallop (Bivalvia, Pectinoida, Pectinidae) between female Chlamysfarreri and male Argopectenirradiansirradians.

Huang X, Bi K, Lu W, Wang S, Zhang L, Bao Z - Comp Cytogenet (2015)

Representative metaphase chromosomes and karyotypes of F1 hybrids of Chlamysfarreri ♀ × Argopectenirradiansirradians ♂ examined by GISH. Chromosomes were labeled by FITC (green) and counterstained by PI (red). In (A, B), chromosomes originated from Argopectenirradiansirradians were painted green using the labeled genomic DNA probes from Argopectenirradiansirradians. In (C, D), chromosomes from Chlamysfarreri were painted green using the labeled genomic DNA probes from Chlamysfarreri. m: metacentric, sm: submetacentric, st: subtelocentric, t: telocentric. Bars = 5 µm.
© Copyright Policy - creative-commons-attribution
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488966&req=5

Figure 1: Representative metaphase chromosomes and karyotypes of F1 hybrids of Chlamysfarreri ♀ × Argopectenirradiansirradians ♂ examined by GISH. Chromosomes were labeled by FITC (green) and counterstained by PI (red). In (A, B), chromosomes originated from Argopectenirradiansirradians were painted green using the labeled genomic DNA probes from Argopectenirradiansirradians. In (C, D), chromosomes from Chlamysfarreri were painted green using the labeled genomic DNA probes from Chlamysfarreri. m: metacentric, sm: submetacentric, st: subtelocentric, t: telocentric. Bars = 5 µm.
Mentions: The chromosome number of hybrids was determined by observing more than one hundred metaphase plates. The statistic results showed that 66.38% of 116 metaphase plates present a diploid component of 2n = 35 in the hybrids. Ten metaphase plates of hybrids were selected to measure arm length and calculate arm ratio and relative length of chromosomes. The karyotype of hybrids is 2n = 35 = 3m + 5sm + 16st + 11t. Typical mitotic spread of the hybrids was shown in Figure 1. The karyotype of Chlamysfarreri is 2n = 38 = 6m + 10sm + 22st (Wang et al. 1990), while that of Argopectenirradiansirradians is 2n = 32 = 10st + 22t (Wang and Guo 2004). Most of the hybrid metaphase plates had a diploid chromosome number of 35, as expected from the parental haploid complements. According to the chromosome configuration, all 3 metacentric chromosomes belonged to Chlamysfarreri, but not Argopectenirradiansirradians. And all 11 telocentric chromosomes, on the contrary, belonged to Argopectenirradiansirradians but not Chlamysfarreri. These chromosome morphological characteristics can be used for chromosome identification in the hybrid metaphases.

Bottom Line: Interspecific crossing was artificially carried out between Chlamysfarreri (Jones & Preston, 1904) ♀ and Argopectenirradiansirradians (Lamarck, 1819) ♂, two of the dominant cultivated scallop species in China.The number of chromosomes in 66.38% of the metaphases was 2n = 35 and the karyotype was 2n = 3 m + 5 sm + 16 st + 11 t.After GISH, two parental genomes were clearly distinguished in hybrids, most of which comprised 19 chromosomes derived from their female parent (Chlamysfarreri) and 16 chromosomes from their male parent (Argopectenirradiansirradians).

View Article: PubMed Central - HTML - PubMed

Affiliation: Key Laboratory of Marine Genetics and Breeding (Ocean University of China), Ministry of Education, Qingdao 266003, China.

ABSTRACT
Interspecific crossing was artificially carried out between Chlamysfarreri (Jones & Preston, 1904) ♀ and Argopectenirradiansirradians (Lamarck, 1819) ♂, two of the dominant cultivated scallop species in China. Genomic in situ hybridization (GISH) was used to examine the chromosome constitution and variation in hybrids at early embryonic stage. The number of chromosomes in 66.38% of the metaphases was 2n = 35 and the karyotype was 2n = 3 m + 5 sm + 16 st + 11 t. After GISH, two parental genomes were clearly distinguished in hybrids, most of which comprised 19 chromosomes derived from their female parent (Chlamysfarreri) and 16 chromosomes from their male parent (Argopectenirradiansirradians). Some chromosome elimination and fragmentation was also observed in the hybrids.

No MeSH data available.


Related in: MedlinePlus