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Karyotype analysis and visualization of 45S rRNA genes using fluorescence in situ hybridization in aroids (Araceae).

Lakshmanan PS, Van Laere K, Eeckhaut T, Van Huylenbroeck J, Van Bockstaele E, Khrustaleva L - Comp Cytogenet (2015)

Bottom Line: B-chromosome-like structures were observed in Anthuriumandraeanum.After FISH experiments, two 45S rDNA sites were observed in 5 genera.Only in Zantedeschiaelliottiana, 4 sites were seen.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute for Agricultural and Fisheries Research (ILVO), Plant Sciences Unit, Applied Genetics and Breeding, Caritasstraat 21, 9090 Melle, Belgium ; Department of Plant Production, Faculty of Bioscience Engineering, Ghent University (UGent), Coupure links 653, 9000 Ghent, Belgium ; Center of Molecular Biotechnology, Department of Genetics and Biotechnology, Russian State Agrarian University-Timiryazev Agricultural Academy (TIMACAD), 49, Timiryazevskaya str., 127550 Moscow, Russia.

ABSTRACT
Karyotype analysis and FISH mapping using 45S rDNA sequences on 6 economically important plant species Anthuriumandraeanum Linden ex André, 1877, Monsteradeliciosa Liebmann, 1849, Philodendronscandens Koch & Sello, 1853, Spathiphyllumwallisii Regel, 1877, Syngoniumauritum (Linnaeus, 1759) Schott, 1829 and Zantedeschiaelliottiana (Knight, 1890) Engler, 1915 within the monocotyledonous family Araceae (aroids) were performed. Chromosome numbers varied between 2n=2x=24 and 2n=2x=60 and the chromosome length varied between 15.77 µm and 1.87 µm. No correlation between chromosome numbers and genome sizes was observed for the studied genera. The chromosome formulas contained only metacentric and submetacentric chromosomes, except for Philodendronscandens in which also telocentric and subtelocentric chromosomes were observed. The highest degree of compaction was calculated for Spathiphyllumwallisii (66.49Mbp/µm). B-chromosome-like structures were observed in Anthuriumandraeanum. Their measured size was 1.87 times smaller than the length of the shortest chromosome. After FISH experiments, two 45S rDNA sites were observed in 5 genera. Only in Zantedeschiaelliottiana, 4 sites were seen. Our results showed clear cytogenetic differences among genera within Araceae, and are the first molecular cytogenetics report for these genera. These chromosome data and molecular cytogenetic information are useful in aroid breeding programmes, systematics and evolutionary studies.

No MeSH data available.


DAPI stained mitotic metaphases with FISH signal: AAnthuriumandraeanum ‘061’ BMonsteradeliciosa ‘Variegata’ CPhilodendronscandensDSpathiphyllumwallisii ‘Domino’ ESyngoniumauritum; and FZantedeschiaelliottiana ‘068’. 45S rDNA FISH signals are indicated by arrows. 45S rDNA sites were observed using FITC (green A–E) and using CY3 (red F).
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Figure 1: DAPI stained mitotic metaphases with FISH signal: AAnthuriumandraeanum ‘061’ BMonsteradeliciosa ‘Variegata’ CPhilodendronscandensDSpathiphyllumwallisii ‘Domino’ ESyngoniumauritum; and FZantedeschiaelliottiana ‘068’. 45S rDNA FISH signals are indicated by arrows. 45S rDNA sites were observed using FITC (green A–E) and using CY3 (red F).

Mentions: The results for genome size measurements and karyotype analysis are summarized in Table 1. Metaphases are shown in Fig. 1 and the idiograms in Fig. 2. Flow cytometric analysis showed the small genome size for Zantedeschiaelliottiana, Philodendronscandens and Syngoniumauritum while for Spathiphyllumwallisii the largest genome size (7.39 ± 0.04 pg/1C) was observed. Monsteradeliciosa (2n=60) had the highest chromosome number. The lowest chromosome number was found in Syngoniumauritum (2n=24). The total chromosome length at haploid chromosome level varied between 147.14 ± 0.39 µm and 44.64 ± 0.52 µm. Compared to the other species Anthuriumandraeanum and Spathiphyllumwallisii had the biggest chromosomes. Philodendronscandens possessed the smallest chromosomes and was the only species that contains subtelocentric and telocentric chromosomes besides metacentric and submetacentric chromosomes (Fig. 2). This is reflected in the asymmetry index (AI) for Philodendronscandens (Table 1). The degree of compaction was the highest in Spathiphyllumwallisii and the lowest in Zantedeschiaelliottiana.


Karyotype analysis and visualization of 45S rRNA genes using fluorescence in situ hybridization in aroids (Araceae).

Lakshmanan PS, Van Laere K, Eeckhaut T, Van Huylenbroeck J, Van Bockstaele E, Khrustaleva L - Comp Cytogenet (2015)

DAPI stained mitotic metaphases with FISH signal: AAnthuriumandraeanum ‘061’ BMonsteradeliciosa ‘Variegata’ CPhilodendronscandensDSpathiphyllumwallisii ‘Domino’ ESyngoniumauritum; and FZantedeschiaelliottiana ‘068’. 45S rDNA FISH signals are indicated by arrows. 45S rDNA sites were observed using FITC (green A–E) and using CY3 (red F).
© Copyright Policy - creative-commons-attribution
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488963&req=5

Figure 1: DAPI stained mitotic metaphases with FISH signal: AAnthuriumandraeanum ‘061’ BMonsteradeliciosa ‘Variegata’ CPhilodendronscandensDSpathiphyllumwallisii ‘Domino’ ESyngoniumauritum; and FZantedeschiaelliottiana ‘068’. 45S rDNA FISH signals are indicated by arrows. 45S rDNA sites were observed using FITC (green A–E) and using CY3 (red F).
Mentions: The results for genome size measurements and karyotype analysis are summarized in Table 1. Metaphases are shown in Fig. 1 and the idiograms in Fig. 2. Flow cytometric analysis showed the small genome size for Zantedeschiaelliottiana, Philodendronscandens and Syngoniumauritum while for Spathiphyllumwallisii the largest genome size (7.39 ± 0.04 pg/1C) was observed. Monsteradeliciosa (2n=60) had the highest chromosome number. The lowest chromosome number was found in Syngoniumauritum (2n=24). The total chromosome length at haploid chromosome level varied between 147.14 ± 0.39 µm and 44.64 ± 0.52 µm. Compared to the other species Anthuriumandraeanum and Spathiphyllumwallisii had the biggest chromosomes. Philodendronscandens possessed the smallest chromosomes and was the only species that contains subtelocentric and telocentric chromosomes besides metacentric and submetacentric chromosomes (Fig. 2). This is reflected in the asymmetry index (AI) for Philodendronscandens (Table 1). The degree of compaction was the highest in Spathiphyllumwallisii and the lowest in Zantedeschiaelliottiana.

Bottom Line: B-chromosome-like structures were observed in Anthuriumandraeanum.After FISH experiments, two 45S rDNA sites were observed in 5 genera.Only in Zantedeschiaelliottiana, 4 sites were seen.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute for Agricultural and Fisheries Research (ILVO), Plant Sciences Unit, Applied Genetics and Breeding, Caritasstraat 21, 9090 Melle, Belgium ; Department of Plant Production, Faculty of Bioscience Engineering, Ghent University (UGent), Coupure links 653, 9000 Ghent, Belgium ; Center of Molecular Biotechnology, Department of Genetics and Biotechnology, Russian State Agrarian University-Timiryazev Agricultural Academy (TIMACAD), 49, Timiryazevskaya str., 127550 Moscow, Russia.

ABSTRACT
Karyotype analysis and FISH mapping using 45S rDNA sequences on 6 economically important plant species Anthuriumandraeanum Linden ex André, 1877, Monsteradeliciosa Liebmann, 1849, Philodendronscandens Koch & Sello, 1853, Spathiphyllumwallisii Regel, 1877, Syngoniumauritum (Linnaeus, 1759) Schott, 1829 and Zantedeschiaelliottiana (Knight, 1890) Engler, 1915 within the monocotyledonous family Araceae (aroids) were performed. Chromosome numbers varied between 2n=2x=24 and 2n=2x=60 and the chromosome length varied between 15.77 µm and 1.87 µm. No correlation between chromosome numbers and genome sizes was observed for the studied genera. The chromosome formulas contained only metacentric and submetacentric chromosomes, except for Philodendronscandens in which also telocentric and subtelocentric chromosomes were observed. The highest degree of compaction was calculated for Spathiphyllumwallisii (66.49Mbp/µm). B-chromosome-like structures were observed in Anthuriumandraeanum. Their measured size was 1.87 times smaller than the length of the shortest chromosome. After FISH experiments, two 45S rDNA sites were observed in 5 genera. Only in Zantedeschiaelliottiana, 4 sites were seen. Our results showed clear cytogenetic differences among genera within Araceae, and are the first molecular cytogenetics report for these genera. These chromosome data and molecular cytogenetic information are useful in aroid breeding programmes, systematics and evolutionary studies.

No MeSH data available.