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QiShenYiQi Pills, a compound in Chinese medicine, protects against pressure overload-induced cardiac hypertrophy through a multi-component and multi-target mode.

Chen YY, Li Q, Pan CS, Yan L, Fan JY, He K, Sun K, Liu YY, Chen QF, Bai Y, Wang CS, He B, Lv AP, Han JY - Sci Rep (2015)

Bottom Line: QSYQ markedly attenuated CH, as evidenced by echocardiography, morphology and biochemistry.Each ingredient alone or their combinations exhibited similar effects as QSYQ but to a lesser extent and differently with astragaloside IV and notoginsenoside R1 being more effective for enhancing energy metabolism, 3, 4-dihydroxy-phenyl lactic acid more effective for counteracting oxidative stress while dalbergia odorifera having little effect on the variables evaluated.In conclusion, QSYQ exerts a more potent antihypertrophic effect than any of its ingredients or their combinations, due to the interaction of its active components through a multi-component and multi-target mode.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China [2] Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China [3] Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China [4] Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of China, Beijing, China.

ABSTRACT
The present study aimed to explore the holistic mechanism for the antihypertrophic effect of a compound in Chinese medicine, QiShenYiQi Pills (QSYQ) and the contributions of its components to the effect in rats with cardiac hypertrophy (CH). After induction of CH by ascending aortic stenosis, rats were treated with QSYQ, each identified active ingredient (astragaloside IV, 3, 4-dihydroxy-phenyl lactic acid or notoginsenoside R1) from its 3 major herb components or dalbergia odorifera, either alone or combinations, for 1 month. QSYQ markedly attenuated CH, as evidenced by echocardiography, morphology and biochemistry. Proteomic analysis and western blot showed that the majority of differentially expressed proteins in the heart of QSYQ-treated rats were associated with energy metabolism or oxidative stress. Each ingredient alone or their combinations exhibited similar effects as QSYQ but to a lesser extent and differently with astragaloside IV and notoginsenoside R1 being more effective for enhancing energy metabolism, 3, 4-dihydroxy-phenyl lactic acid more effective for counteracting oxidative stress while dalbergia odorifera having little effect on the variables evaluated. In conclusion, QSYQ exerts a more potent antihypertrophic effect than any of its ingredients or their combinations, due to the interaction of its active components through a multi-component and multi-target mode.

No MeSH data available.


Related in: MedlinePlus

Western blot of proteins related to energy metabolism pathway in different groups.a The representative western blotting bands of each protein in different groups. b–g The semi-quantitative analysis of ALDOA (b), ENOα (c) ENOβ (d), ECH1 (e), HIF1 (f) and HSP70 (g) in various groups. The bands of ALDOA, ENOβ, HIF1 and GAPDH-2 were cropped from one gel, while the bands of ECH1, ENOα, HSP70 and GAPDH-1 were cropped from another gel, as shown in Supplementary Fig. S5a with indication of molecular size. The quantification was based on the data of 3 independent experiments and normalized to GAPDH. The data are presented as mean ± S.E. *p < 0.05 vs. Sham, #p < 0.05 vs. AAS1M, †p < 0.05 vs. AAS2M, ‡p < 0.05 vs. QSYQ, $p < 0.05 vs. ASIV, §p < 0.05 vs. DLA, €p < 0.05 vs. R1, Ψp < 0.05 vs. DO. n = 4.
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f5: Western blot of proteins related to energy metabolism pathway in different groups.a The representative western blotting bands of each protein in different groups. b–g The semi-quantitative analysis of ALDOA (b), ENOα (c) ENOβ (d), ECH1 (e), HIF1 (f) and HSP70 (g) in various groups. The bands of ALDOA, ENOβ, HIF1 and GAPDH-2 were cropped from one gel, while the bands of ECH1, ENOα, HSP70 and GAPDH-1 were cropped from another gel, as shown in Supplementary Fig. S5a with indication of molecular size. The quantification was based on the data of 3 independent experiments and normalized to GAPDH. The data are presented as mean ± S.E. *p < 0.05 vs. Sham, #p < 0.05 vs. AAS1M, †p < 0.05 vs. AAS2M, ‡p < 0.05 vs. QSYQ, $p < 0.05 vs. ASIV, §p < 0.05 vs. DLA, €p < 0.05 vs. R1, Ψp < 0.05 vs. DO. n = 4.

Mentions: The expressions of 6 enzymes (ALDOA, ENOα, ENOβ, ECH1, HIF1, HSP70) related to energy metabolism were assessed by western blot in different groups. As shown in Fig. 5, AAS challenge led to an increase in the expression of 4 enzymes, including ALDOA, ENOα, HIF1, and HSP70, while a decrease in the expression of ENOβ and ECH1, with AAS2M impairing these proteins more than AAS1M except ECH1 and HSP70. QSYQ restored all the AAS-induced alterations significantly. Mono and combination therapies affected the expression of energy metabolism-related proteins differently depending on the enzyme concerned. For example, ASIV mono-therapy and the ASIV containing combinations inhibited the elevation of ALDOA after AAS, which was intensified by combination with DLA or DLA+R1, while DLA, R1, DO alone and their combinations showed no effect on ALDOA (Fig. 5b). On the other hand, mono-therapy ASIV, DLA or R1 and all the combination therapies showed nearly equally beneficial role for ENOα (Fig. 5c). ASIV, DLA alone and all the combination therapies displayed a beneficial role, but R1 and DO had no effect, in the case of ENOβ (Fig. 5d). ASIV, R1 alone and all combinations except ASIV+DLA increased the expression of ECH1 (Fig. 5e). Interestingly, R1 alone and all the combination therapies showed significant inhibition on HIF1 and HSP70 (Fig. 5f,g). Of notice, in any case, DO alone did not show any effect while QSYQ was always among the most effective treatments.


QiShenYiQi Pills, a compound in Chinese medicine, protects against pressure overload-induced cardiac hypertrophy through a multi-component and multi-target mode.

Chen YY, Li Q, Pan CS, Yan L, Fan JY, He K, Sun K, Liu YY, Chen QF, Bai Y, Wang CS, He B, Lv AP, Han JY - Sci Rep (2015)

Western blot of proteins related to energy metabolism pathway in different groups.a The representative western blotting bands of each protein in different groups. b–g The semi-quantitative analysis of ALDOA (b), ENOα (c) ENOβ (d), ECH1 (e), HIF1 (f) and HSP70 (g) in various groups. The bands of ALDOA, ENOβ, HIF1 and GAPDH-2 were cropped from one gel, while the bands of ECH1, ENOα, HSP70 and GAPDH-1 were cropped from another gel, as shown in Supplementary Fig. S5a with indication of molecular size. The quantification was based on the data of 3 independent experiments and normalized to GAPDH. The data are presented as mean ± S.E. *p < 0.05 vs. Sham, #p < 0.05 vs. AAS1M, †p < 0.05 vs. AAS2M, ‡p < 0.05 vs. QSYQ, $p < 0.05 vs. ASIV, §p < 0.05 vs. DLA, €p < 0.05 vs. R1, Ψp < 0.05 vs. DO. n = 4.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4488877&req=5

f5: Western blot of proteins related to energy metabolism pathway in different groups.a The representative western blotting bands of each protein in different groups. b–g The semi-quantitative analysis of ALDOA (b), ENOα (c) ENOβ (d), ECH1 (e), HIF1 (f) and HSP70 (g) in various groups. The bands of ALDOA, ENOβ, HIF1 and GAPDH-2 were cropped from one gel, while the bands of ECH1, ENOα, HSP70 and GAPDH-1 were cropped from another gel, as shown in Supplementary Fig. S5a with indication of molecular size. The quantification was based on the data of 3 independent experiments and normalized to GAPDH. The data are presented as mean ± S.E. *p < 0.05 vs. Sham, #p < 0.05 vs. AAS1M, †p < 0.05 vs. AAS2M, ‡p < 0.05 vs. QSYQ, $p < 0.05 vs. ASIV, §p < 0.05 vs. DLA, €p < 0.05 vs. R1, Ψp < 0.05 vs. DO. n = 4.
Mentions: The expressions of 6 enzymes (ALDOA, ENOα, ENOβ, ECH1, HIF1, HSP70) related to energy metabolism were assessed by western blot in different groups. As shown in Fig. 5, AAS challenge led to an increase in the expression of 4 enzymes, including ALDOA, ENOα, HIF1, and HSP70, while a decrease in the expression of ENOβ and ECH1, with AAS2M impairing these proteins more than AAS1M except ECH1 and HSP70. QSYQ restored all the AAS-induced alterations significantly. Mono and combination therapies affected the expression of energy metabolism-related proteins differently depending on the enzyme concerned. For example, ASIV mono-therapy and the ASIV containing combinations inhibited the elevation of ALDOA after AAS, which was intensified by combination with DLA or DLA+R1, while DLA, R1, DO alone and their combinations showed no effect on ALDOA (Fig. 5b). On the other hand, mono-therapy ASIV, DLA or R1 and all the combination therapies showed nearly equally beneficial role for ENOα (Fig. 5c). ASIV, DLA alone and all the combination therapies displayed a beneficial role, but R1 and DO had no effect, in the case of ENOβ (Fig. 5d). ASIV, R1 alone and all combinations except ASIV+DLA increased the expression of ECH1 (Fig. 5e). Interestingly, R1 alone and all the combination therapies showed significant inhibition on HIF1 and HSP70 (Fig. 5f,g). Of notice, in any case, DO alone did not show any effect while QSYQ was always among the most effective treatments.

Bottom Line: QSYQ markedly attenuated CH, as evidenced by echocardiography, morphology and biochemistry.Each ingredient alone or their combinations exhibited similar effects as QSYQ but to a lesser extent and differently with astragaloside IV and notoginsenoside R1 being more effective for enhancing energy metabolism, 3, 4-dihydroxy-phenyl lactic acid more effective for counteracting oxidative stress while dalbergia odorifera having little effect on the variables evaluated.In conclusion, QSYQ exerts a more potent antihypertrophic effect than any of its ingredients or their combinations, due to the interaction of its active components through a multi-component and multi-target mode.

View Article: PubMed Central - PubMed

Affiliation: 1] Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China [2] Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China [3] Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing, China [4] Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of China, Beijing, China.

ABSTRACT
The present study aimed to explore the holistic mechanism for the antihypertrophic effect of a compound in Chinese medicine, QiShenYiQi Pills (QSYQ) and the contributions of its components to the effect in rats with cardiac hypertrophy (CH). After induction of CH by ascending aortic stenosis, rats were treated with QSYQ, each identified active ingredient (astragaloside IV, 3, 4-dihydroxy-phenyl lactic acid or notoginsenoside R1) from its 3 major herb components or dalbergia odorifera, either alone or combinations, for 1 month. QSYQ markedly attenuated CH, as evidenced by echocardiography, morphology and biochemistry. Proteomic analysis and western blot showed that the majority of differentially expressed proteins in the heart of QSYQ-treated rats were associated with energy metabolism or oxidative stress. Each ingredient alone or their combinations exhibited similar effects as QSYQ but to a lesser extent and differently with astragaloside IV and notoginsenoside R1 being more effective for enhancing energy metabolism, 3, 4-dihydroxy-phenyl lactic acid more effective for counteracting oxidative stress while dalbergia odorifera having little effect on the variables evaluated. In conclusion, QSYQ exerts a more potent antihypertrophic effect than any of its ingredients or their combinations, due to the interaction of its active components through a multi-component and multi-target mode.

No MeSH data available.


Related in: MedlinePlus