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Identification of urine protein biomarkers with the potential for early detection of lung cancer.

Zhang H, Cao J, Li L, Liu Y, Zhao H, Li N, Li B, Zhang A, Huang H, Chen S, Dong M, Yu L, Zhang J, Chen L - Sci Rep (2015)

Bottom Line: Marked differences at the proteomic level were also detected between the urine of patients and that of healthy population controls.Importantly, we identified 7 proteins commonly found to be significantly up-regulated in both tumor-bearing mice and patients.These tumor biomarkers could potentially aid in the early detection of lung cancer.

View Article: PubMed Central - PubMed

Affiliation: School of Life Science, Tsinghua University, Beijing 100084, China.

ABSTRACT
Lung cancer is the leading cause of cancer-related deaths and has an overall 5-year survival rate lower than 15%. Large-scale clinical trials have demonstrated a significant relative reduction in mortality in high-risk individuals with low-dose computed tomography screening. However, biomarkers capable of identifying the most at-risk population and detecting lung cancer before it becomes clinically apparent are urgently needed in the clinic. Here, we report the identification of urine biomarkers capable of detecting lung cancer. Using the well-characterized inducible Kras (G12D) mouse model of lung cancer, we identified alterations in the urine proteome in tumor-bearing mice compared with sibling controls. Marked differences at the proteomic level were also detected between the urine of patients and that of healthy population controls. Importantly, we identified 7 proteins commonly found to be significantly up-regulated in both tumor-bearing mice and patients. In an independent cohort, we showed that 2 of the 7 proteins were up-regulated in urine samples from lung cancer patients but not in those from controls. The kinetics of these proteins correlated with the disease state in the mouse model. These tumor biomarkers could potentially aid in the early detection of lung cancer.

No MeSH data available.


Related in: MedlinePlus

Bioinformatics analysis of the 7 commonly up-regulated proteins.A,B. MS/MS spectra representing A1BG and LRG1 identified in mouse (left panel) and human (right panel) urine. C. Heatmap of 119 common proteins between 3 groups of mice and 4 groups of human urine proteomic analysis. D. STRING analysis shows the interaction network of the 7 up-regulated proteins.
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f4: Bioinformatics analysis of the 7 commonly up-regulated proteins.A,B. MS/MS spectra representing A1BG and LRG1 identified in mouse (left panel) and human (right panel) urine. C. Heatmap of 119 common proteins between 3 groups of mice and 4 groups of human urine proteomic analysis. D. STRING analysis shows the interaction network of the 7 up-regulated proteins.

Mentions: Based on our mass spectrometry data, A1BG and LRG1 were among the most markedly up-regulated proteins in the urine samples from tumor-bearing mice. Both proteins were likewise up-regulated in the urine of lung cancer patients (Fig. 4A,B). Interestingly, these two proteins have previously been reported to be potential biomarkers of lung cancer28. This finding suggested that the up-regulated urine proteins common to both the tumor-bearing mice and the lung cancer patients were potential biomarkers.


Identification of urine protein biomarkers with the potential for early detection of lung cancer.

Zhang H, Cao J, Li L, Liu Y, Zhao H, Li N, Li B, Zhang A, Huang H, Chen S, Dong M, Yu L, Zhang J, Chen L - Sci Rep (2015)

Bioinformatics analysis of the 7 commonly up-regulated proteins.A,B. MS/MS spectra representing A1BG and LRG1 identified in mouse (left panel) and human (right panel) urine. C. Heatmap of 119 common proteins between 3 groups of mice and 4 groups of human urine proteomic analysis. D. STRING analysis shows the interaction network of the 7 up-regulated proteins.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488871&req=5

f4: Bioinformatics analysis of the 7 commonly up-regulated proteins.A,B. MS/MS spectra representing A1BG and LRG1 identified in mouse (left panel) and human (right panel) urine. C. Heatmap of 119 common proteins between 3 groups of mice and 4 groups of human urine proteomic analysis. D. STRING analysis shows the interaction network of the 7 up-regulated proteins.
Mentions: Based on our mass spectrometry data, A1BG and LRG1 were among the most markedly up-regulated proteins in the urine samples from tumor-bearing mice. Both proteins were likewise up-regulated in the urine of lung cancer patients (Fig. 4A,B). Interestingly, these two proteins have previously been reported to be potential biomarkers of lung cancer28. This finding suggested that the up-regulated urine proteins common to both the tumor-bearing mice and the lung cancer patients were potential biomarkers.

Bottom Line: Marked differences at the proteomic level were also detected between the urine of patients and that of healthy population controls.Importantly, we identified 7 proteins commonly found to be significantly up-regulated in both tumor-bearing mice and patients.These tumor biomarkers could potentially aid in the early detection of lung cancer.

View Article: PubMed Central - PubMed

Affiliation: School of Life Science, Tsinghua University, Beijing 100084, China.

ABSTRACT
Lung cancer is the leading cause of cancer-related deaths and has an overall 5-year survival rate lower than 15%. Large-scale clinical trials have demonstrated a significant relative reduction in mortality in high-risk individuals with low-dose computed tomography screening. However, biomarkers capable of identifying the most at-risk population and detecting lung cancer before it becomes clinically apparent are urgently needed in the clinic. Here, we report the identification of urine biomarkers capable of detecting lung cancer. Using the well-characterized inducible Kras (G12D) mouse model of lung cancer, we identified alterations in the urine proteome in tumor-bearing mice compared with sibling controls. Marked differences at the proteomic level were also detected between the urine of patients and that of healthy population controls. Importantly, we identified 7 proteins commonly found to be significantly up-regulated in both tumor-bearing mice and patients. In an independent cohort, we showed that 2 of the 7 proteins were up-regulated in urine samples from lung cancer patients but not in those from controls. The kinetics of these proteins correlated with the disease state in the mouse model. These tumor biomarkers could potentially aid in the early detection of lung cancer.

No MeSH data available.


Related in: MedlinePlus