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Uncovering miRNAs involved in crosstalk between nutrient deficiencies in Arabidopsis.

Liang G, Ai Q, Yu D - Sci Rep (2015)

Bottom Line: In particular, the miRNAs that are induced specifically by a certain nutrient deficiency are often suppressed by other nutrient deficiencies.Further investigation indicated that the modulation of nutrient-responsive miRNA abundance affects the adaptation of plants to nutrient starvation conditions.This study revealed that miRNAs function as important regulatory nodes of different nutrient metabolic pathways.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan 650223, China.

ABSTRACT
Integrating carbon (C), nitrogen (N), and sulfur (S) metabolism is essential for the growth and development of living organisms. MicroRNAs (miRNAs) play key roles in regulating nutrient metabolism in plants. However, how plant miRNAs mediate crosstalk between different nutrient metabolic pathways is unclear. In this study, deep sequencing of Arabidopsis thaliana small RNAs was used to reveal miRNAs that were differentially expressed in response to C, N, or S deficiency. Comparative analysis revealed that the targets of the differentially expressed miRNAs are involved in different cellular responses and metabolic processes, including transcriptional regulation, auxin signal transduction, nutrient homeostasis, and regulation of development. C, N, and S deficiency specifically induced miR169b/c, miR826 and miR395, respectively. In contrast, miR167, miR172, miR397, miR398, miR399, miR408, miR775, miR827, miR841, miR857, and miR2111 are commonly suppressed by C, N, and S deficiency. In particular, the miRNAs that are induced specifically by a certain nutrient deficiency are often suppressed by other nutrient deficiencies. Further investigation indicated that the modulation of nutrient-responsive miRNA abundance affects the adaptation of plants to nutrient starvation conditions. This study revealed that miRNAs function as important regulatory nodes of different nutrient metabolic pathways.

No MeSH data available.


Related in: MedlinePlus

Phenotypes of transgenic plants under nutrient starvation conditions.(A) Ten-day-old seedlings grown vertically. (B) The ratio of shoot/root mass of 10-day-old seedlings grown under full nutrient and nutrient-deficient conditions. The error bars represent the SD from triplicate samples with each sample containing 10–15 plants. Student’s t test indicated that the values marked by one asterisk are significantly different from the corresponding full nutrient value (P < 0.01; n = 3).
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f4: Phenotypes of transgenic plants under nutrient starvation conditions.(A) Ten-day-old seedlings grown vertically. (B) The ratio of shoot/root mass of 10-day-old seedlings grown under full nutrient and nutrient-deficient conditions. The error bars represent the SD from triplicate samples with each sample containing 10–15 plants. Student’s t test indicated that the values marked by one asterisk are significantly different from the corresponding full nutrient value (P < 0.01; n = 3).

Mentions: Overexpression of a certain nutrient-responsive miRNA altered the plant’s adaptation to nutrient starvation conditions21222527. To investigate the functions of nutrient-responsive miRNAs in nutrient starvation adaptation, miRNA overexpression plants (miR160a-ox, miR395a-ox, and miR399b-ox) and miR160 suppression plants (STTM160) were used to evaluate phenotypes in nutrient starvation conditions. As shown in Fig. 4A, miR160a-ox, STTM160, and miR399b-ox plants produced short primary roots compared with wild-type plants under –C conditions. Under –N conditions, miR399b-ox plants developed lateral roots, whereas the wild-type and the other transgenic plants had no visible lateral roots. Under –S conditions, the lateral roots of miR160-ox and miR395a plants were longer than the other plants. The shoot:root ratio is one measure to assess the growth status of plants. The shoot:root ratio of miR160a-ox plants was lower under –C and –S conditions, but higher under –N conditions compared with the other plants. STTM160 and miR399b plants had lower shoot:root ratios than the other plants under –C and –S conditions. In contrast, miR395a-ox plants only displayed a differential shoot:root ratio under –S conditions. These results suggested that misexpression of nutrient-responsive miRNAs leads to altered adaptation to nutrient deficiency.


Uncovering miRNAs involved in crosstalk between nutrient deficiencies in Arabidopsis.

Liang G, Ai Q, Yu D - Sci Rep (2015)

Phenotypes of transgenic plants under nutrient starvation conditions.(A) Ten-day-old seedlings grown vertically. (B) The ratio of shoot/root mass of 10-day-old seedlings grown under full nutrient and nutrient-deficient conditions. The error bars represent the SD from triplicate samples with each sample containing 10–15 plants. Student’s t test indicated that the values marked by one asterisk are significantly different from the corresponding full nutrient value (P < 0.01; n = 3).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488870&req=5

f4: Phenotypes of transgenic plants under nutrient starvation conditions.(A) Ten-day-old seedlings grown vertically. (B) The ratio of shoot/root mass of 10-day-old seedlings grown under full nutrient and nutrient-deficient conditions. The error bars represent the SD from triplicate samples with each sample containing 10–15 plants. Student’s t test indicated that the values marked by one asterisk are significantly different from the corresponding full nutrient value (P < 0.01; n = 3).
Mentions: Overexpression of a certain nutrient-responsive miRNA altered the plant’s adaptation to nutrient starvation conditions21222527. To investigate the functions of nutrient-responsive miRNAs in nutrient starvation adaptation, miRNA overexpression plants (miR160a-ox, miR395a-ox, and miR399b-ox) and miR160 suppression plants (STTM160) were used to evaluate phenotypes in nutrient starvation conditions. As shown in Fig. 4A, miR160a-ox, STTM160, and miR399b-ox plants produced short primary roots compared with wild-type plants under –C conditions. Under –N conditions, miR399b-ox plants developed lateral roots, whereas the wild-type and the other transgenic plants had no visible lateral roots. Under –S conditions, the lateral roots of miR160-ox and miR395a plants were longer than the other plants. The shoot:root ratio is one measure to assess the growth status of plants. The shoot:root ratio of miR160a-ox plants was lower under –C and –S conditions, but higher under –N conditions compared with the other plants. STTM160 and miR399b plants had lower shoot:root ratios than the other plants under –C and –S conditions. In contrast, miR395a-ox plants only displayed a differential shoot:root ratio under –S conditions. These results suggested that misexpression of nutrient-responsive miRNAs leads to altered adaptation to nutrient deficiency.

Bottom Line: In particular, the miRNAs that are induced specifically by a certain nutrient deficiency are often suppressed by other nutrient deficiencies.Further investigation indicated that the modulation of nutrient-responsive miRNA abundance affects the adaptation of plants to nutrient starvation conditions.This study revealed that miRNAs function as important regulatory nodes of different nutrient metabolic pathways.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan 650223, China.

ABSTRACT
Integrating carbon (C), nitrogen (N), and sulfur (S) metabolism is essential for the growth and development of living organisms. MicroRNAs (miRNAs) play key roles in regulating nutrient metabolism in plants. However, how plant miRNAs mediate crosstalk between different nutrient metabolic pathways is unclear. In this study, deep sequencing of Arabidopsis thaliana small RNAs was used to reveal miRNAs that were differentially expressed in response to C, N, or S deficiency. Comparative analysis revealed that the targets of the differentially expressed miRNAs are involved in different cellular responses and metabolic processes, including transcriptional regulation, auxin signal transduction, nutrient homeostasis, and regulation of development. C, N, and S deficiency specifically induced miR169b/c, miR826 and miR395, respectively. In contrast, miR167, miR172, miR397, miR398, miR399, miR408, miR775, miR827, miR841, miR857, and miR2111 are commonly suppressed by C, N, and S deficiency. In particular, the miRNAs that are induced specifically by a certain nutrient deficiency are often suppressed by other nutrient deficiencies. Further investigation indicated that the modulation of nutrient-responsive miRNA abundance affects the adaptation of plants to nutrient starvation conditions. This study revealed that miRNAs function as important regulatory nodes of different nutrient metabolic pathways.

No MeSH data available.


Related in: MedlinePlus