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Controlled electromechanical cell stimulation on-a-chip.

Pavesi A, Adriani G, Rasponi M, Zervantonakis IK, Fiore GB, Kamm RD - Sci Rep (2015)

Bottom Line: The platform was validated in experiments using human bone marrow mesenchymal stem cells.These experiments demonstrated the ability for inducing changes in cell morphology, cytoskeletal fiber orientation and changes in gene expression under physiological stimuli.This novel bioengineering approach can be readily applied to various studies, especially in the fields of stem cell biology and regenerative medicine.

View Article: PubMed Central - PubMed

Affiliation: Biosym IRG, Singapore-MIT Alliance for Research and Technology, Singapore.

ABSTRACT
Stem cell research has yielded promising advances in regenerative medicine, but standard assays generally lack the ability to combine different cell stimulations with rapid sample processing and precise fluid control. In this work, we describe the design and fabrication of a micro-scale cell stimulator capable of simultaneously providing mechanical, electrical, and biochemical stimulation, and subsequently extracting detailed morphological and gene-expression analysis on the cellular response. This micro-device offers the opportunity to overcome previous limitations and recreate critical elements of the in vivo microenvironment in order to investigate cellular responses to three different stimulations. The platform was validated in experiments using human bone marrow mesenchymal stem cells. These experiments demonstrated the ability for inducing changes in cell morphology, cytoskeletal fiber orientation and changes in gene expression under physiological stimuli. This novel bioengineering approach can be readily applied to various studies, especially in the fields of stem cell biology and regenerative medicine.

No MeSH data available.


Related in: MedlinePlus

Design of the microfluidic platform developed to investigate the biological cell responses to various stimuli.A) Schematic view of the device for applying electrical, mechanical and chemical stimulations. The central channel (in red) is the media channel to provide nutrients and soluble factors to cells. The pneumatic channels (in light blue) perform mechanical stimulation by stretching the PDMS membrane (yellow arrows) where the cells are cultured. The electrical layer contains two conductive regions composed of a mixture of CNTs and PDMS (in light gray), which are connected to the stimulator through two external gold-coated connectors (in red and black). The uniform electric field across the cell culture region is represented by the red arrows. (B) Cross section of the device in the unstimulated configuration. (C) Cross section of the device in the electromechanical stimulated configuration. Applying vacuum in the two lateral pneumatic channels (in light blue) allows stretching of the cells on the deformable membrane (in yellow).
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f1: Design of the microfluidic platform developed to investigate the biological cell responses to various stimuli.A) Schematic view of the device for applying electrical, mechanical and chemical stimulations. The central channel (in red) is the media channel to provide nutrients and soluble factors to cells. The pneumatic channels (in light blue) perform mechanical stimulation by stretching the PDMS membrane (yellow arrows) where the cells are cultured. The electrical layer contains two conductive regions composed of a mixture of CNTs and PDMS (in light gray), which are connected to the stimulator through two external gold-coated connectors (in red and black). The uniform electric field across the cell culture region is represented by the red arrows. (B) Cross section of the device in the unstimulated configuration. (C) Cross section of the device in the electromechanical stimulated configuration. Applying vacuum in the two lateral pneumatic channels (in light blue) allows stretching of the cells on the deformable membrane (in yellow).

Mentions: Polydimethylsiloxane (PDMS) was chosen as the main material for the production of the micro-bioreactor (Fig. 1) due to its favorable features in cell culture applications (namely gas permeability and optical transparency) and the robustness of soft-lithography techniques. In addition, its elastic mechanical properties were exploited to apply controlled strains to cells, while the ability to embed electrical paths by locally doping the PDMS pre-polymer with carbon nanotubes (CNTs)17 was used to deliver electrical stimulation.


Controlled electromechanical cell stimulation on-a-chip.

Pavesi A, Adriani G, Rasponi M, Zervantonakis IK, Fiore GB, Kamm RD - Sci Rep (2015)

Design of the microfluidic platform developed to investigate the biological cell responses to various stimuli.A) Schematic view of the device for applying electrical, mechanical and chemical stimulations. The central channel (in red) is the media channel to provide nutrients and soluble factors to cells. The pneumatic channels (in light blue) perform mechanical stimulation by stretching the PDMS membrane (yellow arrows) where the cells are cultured. The electrical layer contains two conductive regions composed of a mixture of CNTs and PDMS (in light gray), which are connected to the stimulator through two external gold-coated connectors (in red and black). The uniform electric field across the cell culture region is represented by the red arrows. (B) Cross section of the device in the unstimulated configuration. (C) Cross section of the device in the electromechanical stimulated configuration. Applying vacuum in the two lateral pneumatic channels (in light blue) allows stretching of the cells on the deformable membrane (in yellow).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488866&req=5

f1: Design of the microfluidic platform developed to investigate the biological cell responses to various stimuli.A) Schematic view of the device for applying electrical, mechanical and chemical stimulations. The central channel (in red) is the media channel to provide nutrients and soluble factors to cells. The pneumatic channels (in light blue) perform mechanical stimulation by stretching the PDMS membrane (yellow arrows) where the cells are cultured. The electrical layer contains two conductive regions composed of a mixture of CNTs and PDMS (in light gray), which are connected to the stimulator through two external gold-coated connectors (in red and black). The uniform electric field across the cell culture region is represented by the red arrows. (B) Cross section of the device in the unstimulated configuration. (C) Cross section of the device in the electromechanical stimulated configuration. Applying vacuum in the two lateral pneumatic channels (in light blue) allows stretching of the cells on the deformable membrane (in yellow).
Mentions: Polydimethylsiloxane (PDMS) was chosen as the main material for the production of the micro-bioreactor (Fig. 1) due to its favorable features in cell culture applications (namely gas permeability and optical transparency) and the robustness of soft-lithography techniques. In addition, its elastic mechanical properties were exploited to apply controlled strains to cells, while the ability to embed electrical paths by locally doping the PDMS pre-polymer with carbon nanotubes (CNTs)17 was used to deliver electrical stimulation.

Bottom Line: The platform was validated in experiments using human bone marrow mesenchymal stem cells.These experiments demonstrated the ability for inducing changes in cell morphology, cytoskeletal fiber orientation and changes in gene expression under physiological stimuli.This novel bioengineering approach can be readily applied to various studies, especially in the fields of stem cell biology and regenerative medicine.

View Article: PubMed Central - PubMed

Affiliation: Biosym IRG, Singapore-MIT Alliance for Research and Technology, Singapore.

ABSTRACT
Stem cell research has yielded promising advances in regenerative medicine, but standard assays generally lack the ability to combine different cell stimulations with rapid sample processing and precise fluid control. In this work, we describe the design and fabrication of a micro-scale cell stimulator capable of simultaneously providing mechanical, electrical, and biochemical stimulation, and subsequently extracting detailed morphological and gene-expression analysis on the cellular response. This micro-device offers the opportunity to overcome previous limitations and recreate critical elements of the in vivo microenvironment in order to investigate cellular responses to three different stimulations. The platform was validated in experiments using human bone marrow mesenchymal stem cells. These experiments demonstrated the ability for inducing changes in cell morphology, cytoskeletal fiber orientation and changes in gene expression under physiological stimuli. This novel bioengineering approach can be readily applied to various studies, especially in the fields of stem cell biology and regenerative medicine.

No MeSH data available.


Related in: MedlinePlus