Limits...
Systematic characterization of the peroxidase gene family provides new insights into fungal pathogenicity in Magnaporthe oryzae.

Mir AA, Park SY, Abu Sadat M, Kim S, Choi J, Jeon J, Lee YH - Sci Rep (2015)

Bottom Line: We identified significant and positive correlations among sensitivity to H2O2, peroxidase activity and fungal pathogenicity.Transcriptional profiling of other peroxidases in ΔMoprx1 suggested interwoven nature of the peroxidase-mediated antioxidant defense system.The results from this study provide insight into the infection strategy built on evolutionarily conserved peroxidases in the rice blast fungus.

View Article: PubMed Central - PubMed

Affiliation: Department of Agricultural Biotechnology, Fungal Bioinformatics Laboratory, Center for Fungal Genetic Resources, and Center for Fungal Pathogenesis, Seoul National University, Seoul 151-921, Korea.

ABSTRACT
Fungal pathogens have evolved antioxidant defense against reactive oxygen species produced as a part of host innate immunity. Recent studies proposed peroxidases as components of antioxidant defense system. However, the role of fungal peroxidases during interaction with host plants has not been explored at the genomic level. Here, we systematically identified peroxidase genes and analyzed their impact on fungal pathogenesis in a model plant pathogenic fungus, Magnaporthe oryzae. Phylogeny reconstruction placed 27 putative peroxidase genes into 15 clades. Expression profiles showed that majority of them are responsive to in planta condition and in vitro H2O2. Our analysis of individual deletion mutants for seven selected genes including MoPRX1 revealed that these genes contribute to fungal development and/or pathogenesis. We identified significant and positive correlations among sensitivity to H2O2, peroxidase activity and fungal pathogenicity. In-depth analysis of MoPRX1 demonstrated that it is a functional ortholog of thioredoxin peroxidase in Saccharomyces cerevisiae and is required for detoxification of the oxidative burst within host cells. Transcriptional profiling of other peroxidases in ΔMoprx1 suggested interwoven nature of the peroxidase-mediated antioxidant defense system. The results from this study provide insight into the infection strategy built on evolutionarily conserved peroxidases in the rice blast fungus.

No MeSH data available.


Related in: MedlinePlus

Expression profiling of 27 M. oryzae peroxidase genes during infection-related developmental stages and infection (78 hpi) on rice, and under oxidative stress.Upregulated genes (more than 1.5-fold) are indicated by red bars and downregulated genes (less than 0.5-fold) are denoted by blue bars. The genes not showing differential expression are marked in gray. Seven peroxidase genes were selected for functional analysis are highlighted as red.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4488832&req=5

f2: Expression profiling of 27 M. oryzae peroxidase genes during infection-related developmental stages and infection (78 hpi) on rice, and under oxidative stress.Upregulated genes (more than 1.5-fold) are indicated by red bars and downregulated genes (less than 0.5-fold) are denoted by blue bars. The genes not showing differential expression are marked in gray. Seven peroxidase genes were selected for functional analysis are highlighted as red.

Mentions: As a next step, we performed expression profiling of the 27 peroxidase-encoding genes using qRT-PCR during infection-related developmental stages that included conidiation, appressorium formation, and 78 h post incubation (hpi) on rice plants and under oxidative stress with 2.5 mM H2O2 (Fig. 2). Expression analysis revealed that most of the peroxidase genes were differentially expressed under the imposed conditions. Compared to expression in mycelia, only a few genes were upregulated in developmental samples, including the conidia and appressoria. However, we found that a majority of the genes (23 genes or 85.2%) were upregulated during the infection stage at 78 hpi. Fourteen genes (51.9%), the exceptions being MoAPX3, MoLIP2, NOX3, MoHPX2, CATA, MoPRX1, TPX1, MoVPX1 and HYR1, were also upregulated under H2O2 stress conditions (Fig. 2). Such an expression pattern suggested the possibility of peroxidase genes playing roles associated with ROS during plant infection.


Systematic characterization of the peroxidase gene family provides new insights into fungal pathogenicity in Magnaporthe oryzae.

Mir AA, Park SY, Abu Sadat M, Kim S, Choi J, Jeon J, Lee YH - Sci Rep (2015)

Expression profiling of 27 M. oryzae peroxidase genes during infection-related developmental stages and infection (78 hpi) on rice, and under oxidative stress.Upregulated genes (more than 1.5-fold) are indicated by red bars and downregulated genes (less than 0.5-fold) are denoted by blue bars. The genes not showing differential expression are marked in gray. Seven peroxidase genes were selected for functional analysis are highlighted as red.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488832&req=5

f2: Expression profiling of 27 M. oryzae peroxidase genes during infection-related developmental stages and infection (78 hpi) on rice, and under oxidative stress.Upregulated genes (more than 1.5-fold) are indicated by red bars and downregulated genes (less than 0.5-fold) are denoted by blue bars. The genes not showing differential expression are marked in gray. Seven peroxidase genes were selected for functional analysis are highlighted as red.
Mentions: As a next step, we performed expression profiling of the 27 peroxidase-encoding genes using qRT-PCR during infection-related developmental stages that included conidiation, appressorium formation, and 78 h post incubation (hpi) on rice plants and under oxidative stress with 2.5 mM H2O2 (Fig. 2). Expression analysis revealed that most of the peroxidase genes were differentially expressed under the imposed conditions. Compared to expression in mycelia, only a few genes were upregulated in developmental samples, including the conidia and appressoria. However, we found that a majority of the genes (23 genes or 85.2%) were upregulated during the infection stage at 78 hpi. Fourteen genes (51.9%), the exceptions being MoAPX3, MoLIP2, NOX3, MoHPX2, CATA, MoPRX1, TPX1, MoVPX1 and HYR1, were also upregulated under H2O2 stress conditions (Fig. 2). Such an expression pattern suggested the possibility of peroxidase genes playing roles associated with ROS during plant infection.

Bottom Line: We identified significant and positive correlations among sensitivity to H2O2, peroxidase activity and fungal pathogenicity.Transcriptional profiling of other peroxidases in ΔMoprx1 suggested interwoven nature of the peroxidase-mediated antioxidant defense system.The results from this study provide insight into the infection strategy built on evolutionarily conserved peroxidases in the rice blast fungus.

View Article: PubMed Central - PubMed

Affiliation: Department of Agricultural Biotechnology, Fungal Bioinformatics Laboratory, Center for Fungal Genetic Resources, and Center for Fungal Pathogenesis, Seoul National University, Seoul 151-921, Korea.

ABSTRACT
Fungal pathogens have evolved antioxidant defense against reactive oxygen species produced as a part of host innate immunity. Recent studies proposed peroxidases as components of antioxidant defense system. However, the role of fungal peroxidases during interaction with host plants has not been explored at the genomic level. Here, we systematically identified peroxidase genes and analyzed their impact on fungal pathogenesis in a model plant pathogenic fungus, Magnaporthe oryzae. Phylogeny reconstruction placed 27 putative peroxidase genes into 15 clades. Expression profiles showed that majority of them are responsive to in planta condition and in vitro H2O2. Our analysis of individual deletion mutants for seven selected genes including MoPRX1 revealed that these genes contribute to fungal development and/or pathogenesis. We identified significant and positive correlations among sensitivity to H2O2, peroxidase activity and fungal pathogenicity. In-depth analysis of MoPRX1 demonstrated that it is a functional ortholog of thioredoxin peroxidase in Saccharomyces cerevisiae and is required for detoxification of the oxidative burst within host cells. Transcriptional profiling of other peroxidases in ΔMoprx1 suggested interwoven nature of the peroxidase-mediated antioxidant defense system. The results from this study provide insight into the infection strategy built on evolutionarily conserved peroxidases in the rice blast fungus.

No MeSH data available.


Related in: MedlinePlus