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Anti-Inflammatory Effect of Angelica gigas via Heme Oxygenase (HO)-1 Expression.

Cho JH, Kwon JE, Cho Y, Kim I, Kang SC - Nutrients (2015)

Bottom Line: Although decursin had the best NO inhibition and anti-oxidative effects, the effects of AGE treatment far surpassed that of decursin.The expression of HO-1 is an effective indicator of the anti-inflammatory effects of AG.Based on the results of the coumarin compounds, HO-1 expression was found to be dose dependent and specific to decursin.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological and Environmental Science, Dongguk University, Goyang 410-820, Korea. jhcho@dongguk.edu.

ABSTRACT
Angelica gigas (AG) is effective against various medical conditions such as bacterial infection, inflammation, and cancer. It contains a number of coumarin compounds and the group of interest is the pyranocoumarin, which comprises decursin and decursinol angelate. This group has an effect on controlling inflammation, which is caused by excessive nitric oxide (NO) production. Heme oxygenases (HOs), particularly HO-1, play a role in regulating the production of NO. Thus, this study aimed to investigate the anti-inflammatory effects of AG by measuring HO-1 expression. Treatments with CH2Cl2 layer and Angelica gigas extract (AGE) showed the highest NO inhibition effects. Decursin, decursinol angelate, and nodakenin were isolated from the CH2Cl2 layer of AGE. Decursin also demonstrated the highest anti-oxidative effect among the coumarins. Although decursin had the best NO inhibition and anti-oxidative effects, the effects of AGE treatment far surpassed that of decursin. This is owing to the combination effect of the coumarins present within AGE, which is a solvent extract of AG. The expression of HO-1 is an effective indicator of the anti-inflammatory effects of AG. Based on the results of the coumarin compounds, HO-1 expression was found to be dose dependent and specific to decursin.

No MeSH data available.


Related in: MedlinePlus

Isolation scheme for determining constituents of A. gigas with anti-inflammatory properties.
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nutrients-07-04862-f001: Isolation scheme for determining constituents of A. gigas with anti-inflammatory properties.

Mentions: Dried and powdered rhizome of AG (200 g) was extracted with 1 L of 95% ethanol (EtOH extract, AGE) for 24 h at room temperature. Extracts were filtered through Whatman No. 1 filter paper and then concentrated under reduced pressure at 40 °C using a rotary evaporator and stored at 4 °C until use. This crude extract (AGE) was suspended in distilled water and sequentially partitioned with n-hexane, CH2Cl2, EtOAc, and n-BuOH. The CH2Cl2 layer was subjected to silica column chromatography (CC, n-hexane-CH2Cl2-EtOAc, 20:1:1) and then compounds 1 and 2 were separated by C18 reversed-phase HPLC (75% MeOH) and recycling HPLC (JAI-GEL, GS-310) with MeOH as an eluent. The n-BuOH layer was assessed using ODS column chromatography (CC, 45% aq MeOH) and then purified to yield nodakenin (3) (Figure 1). NMR experiments were performed on a Varian Unity INOVA 500 spectrometer with the usual pulse sequences. EIMS data were obtained on an HP 6890 series GC System equipped with a 5973 Mass Selective Detector. Column chromatography was carried out on Si gel 60 (Merck, 230–400 mesh) and Sephadex LH-20 (Lipophilic Sephadex, Sigma-Aldrich, 25–100 µm). HPLC and recycling HPLC were performed using a phenomenex Luna 10 µ C18 (2) (250 × 10 mm) column and JAIGEL-GS310 column, respectively, and a UV detector (254 nm).


Anti-Inflammatory Effect of Angelica gigas via Heme Oxygenase (HO)-1 Expression.

Cho JH, Kwon JE, Cho Y, Kim I, Kang SC - Nutrients (2015)

Isolation scheme for determining constituents of A. gigas with anti-inflammatory properties.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488820&req=5

nutrients-07-04862-f001: Isolation scheme for determining constituents of A. gigas with anti-inflammatory properties.
Mentions: Dried and powdered rhizome of AG (200 g) was extracted with 1 L of 95% ethanol (EtOH extract, AGE) for 24 h at room temperature. Extracts were filtered through Whatman No. 1 filter paper and then concentrated under reduced pressure at 40 °C using a rotary evaporator and stored at 4 °C until use. This crude extract (AGE) was suspended in distilled water and sequentially partitioned with n-hexane, CH2Cl2, EtOAc, and n-BuOH. The CH2Cl2 layer was subjected to silica column chromatography (CC, n-hexane-CH2Cl2-EtOAc, 20:1:1) and then compounds 1 and 2 were separated by C18 reversed-phase HPLC (75% MeOH) and recycling HPLC (JAI-GEL, GS-310) with MeOH as an eluent. The n-BuOH layer was assessed using ODS column chromatography (CC, 45% aq MeOH) and then purified to yield nodakenin (3) (Figure 1). NMR experiments were performed on a Varian Unity INOVA 500 spectrometer with the usual pulse sequences. EIMS data were obtained on an HP 6890 series GC System equipped with a 5973 Mass Selective Detector. Column chromatography was carried out on Si gel 60 (Merck, 230–400 mesh) and Sephadex LH-20 (Lipophilic Sephadex, Sigma-Aldrich, 25–100 µm). HPLC and recycling HPLC were performed using a phenomenex Luna 10 µ C18 (2) (250 × 10 mm) column and JAIGEL-GS310 column, respectively, and a UV detector (254 nm).

Bottom Line: Although decursin had the best NO inhibition and anti-oxidative effects, the effects of AGE treatment far surpassed that of decursin.The expression of HO-1 is an effective indicator of the anti-inflammatory effects of AG.Based on the results of the coumarin compounds, HO-1 expression was found to be dose dependent and specific to decursin.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological and Environmental Science, Dongguk University, Goyang 410-820, Korea. jhcho@dongguk.edu.

ABSTRACT
Angelica gigas (AG) is effective against various medical conditions such as bacterial infection, inflammation, and cancer. It contains a number of coumarin compounds and the group of interest is the pyranocoumarin, which comprises decursin and decursinol angelate. This group has an effect on controlling inflammation, which is caused by excessive nitric oxide (NO) production. Heme oxygenases (HOs), particularly HO-1, play a role in regulating the production of NO. Thus, this study aimed to investigate the anti-inflammatory effects of AG by measuring HO-1 expression. Treatments with CH2Cl2 layer and Angelica gigas extract (AGE) showed the highest NO inhibition effects. Decursin, decursinol angelate, and nodakenin were isolated from the CH2Cl2 layer of AGE. Decursin also demonstrated the highest anti-oxidative effect among the coumarins. Although decursin had the best NO inhibition and anti-oxidative effects, the effects of AGE treatment far surpassed that of decursin. This is owing to the combination effect of the coumarins present within AGE, which is a solvent extract of AG. The expression of HO-1 is an effective indicator of the anti-inflammatory effects of AG. Based on the results of the coumarin compounds, HO-1 expression was found to be dose dependent and specific to decursin.

No MeSH data available.


Related in: MedlinePlus