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Protective Effect of the Total Saponins from Rosa laevigata Michx Fruit against Carbon Tetrachloride-Induced Liver Fibrosis in Rats.

Dong D, Yin L, Qi Y, Xu L, Peng J - Nutrients (2015)

Bottom Line: The results showed that RLTS significantly rehabilitated the levels of alanine aminotransferase, aspartate aminotransferase, malondialdehyde, glutathione, glutathione peroxidase, catalase, superoxide dismutase, hydroxyproline, α-smooth muscle actin, collagen I, collagen III and fibronectin, which were confirmed using H&E, Sirius Red and Masson histopathological assays.Further research indicated that RLTS markedly reduced cytochrome P450 2E1 activity, attenuated oxidative stress, and suppressed inflammation.In addition, RLTS facilitated matrix degradation through down-regulation of matrix metalloproteinase2, matrix metalloproteinase 9 and metalloproteinases1, and exerted the anti-fibrotic effects through affecting transforming growth factor β/Smad, focal adhesion kinase/phosphatidylinositol-3-kinase/amino kinase terminal/70-kDa ribosomal S6 Kinase (FAK-PI3K-Akt-p70(S6K)) and mitogen-activated protein kinase (MAPK) signaling pathways.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Dalian Medical University, No. 9 Western Lvshun South Road, Dalian 116044, China. deshidong@163.com.

ABSTRACT
In this study, the protective effect of the total saponins from Rosa laevigata Michx (RLTS) against liver fibrosis induced by carbon tetrachloride (CCl4) in rats was evaluated. The results showed that RLTS significantly rehabilitated the levels of alanine aminotransferase, aspartate aminotransferase, malondialdehyde, glutathione, glutathione peroxidase, catalase, superoxide dismutase, hydroxyproline, α-smooth muscle actin, collagen I, collagen III and fibronectin, which were confirmed using H&E, Sirius Red and Masson histopathological assays. Further research indicated that RLTS markedly reduced cytochrome P450 2E1 activity, attenuated oxidative stress, and suppressed inflammation. In addition, RLTS facilitated matrix degradation through down-regulation of matrix metalloproteinase2, matrix metalloproteinase 9 and metalloproteinases1, and exerted the anti-fibrotic effects through affecting transforming growth factor β/Smad, focal adhesion kinase/phosphatidylinositol-3-kinase/amino kinase terminal/70-kDa ribosomal S6 Kinase (FAK-PI3K-Akt-p70(S6K)) and mitogen-activated protein kinase (MAPK) signaling pathways. Taken together, our data indicate that RLTS can be applied as one effective candidate for the treatment of liver fibrosis in the future.

No MeSH data available.


Related in: MedlinePlus

Effect of Rosa laevigata Michx fruit (RLTS) on the protein expression of CYP2E1 (A); Effects of RLTS on the levels of MDA, GSH-Px, GSH, CAT and SOD in livers (B–F); Effects of RLTS on the protein expressions of HO-1, SOD2 and Nrf2 (G–I). (I) RLTS control; (II) Normal control; (III) model; (IV) RLTS (70 mg/kg) + CCl4; (V) RLTS (140 mg/kg) + CCl4; (VI) RLTS (210 mg/kg) + CCl4; (VII) silymarin (210 mg/kg) + CCl4. Values are expressed as mean ± SD (n = 4). * p < 0.05; ** p < 0.01 vs. model group.
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nutrients-07-04829-f005: Effect of Rosa laevigata Michx fruit (RLTS) on the protein expression of CYP2E1 (A); Effects of RLTS on the levels of MDA, GSH-Px, GSH, CAT and SOD in livers (B–F); Effects of RLTS on the protein expressions of HO-1, SOD2 and Nrf2 (G–I). (I) RLTS control; (II) Normal control; (III) model; (IV) RLTS (70 mg/kg) + CCl4; (V) RLTS (140 mg/kg) + CCl4; (VI) RLTS (210 mg/kg) + CCl4; (VII) silymarin (210 mg/kg) + CCl4. Values are expressed as mean ± SD (n = 4). * p < 0.05; ** p < 0.01 vs. model group.

Mentions: As shown in Figure 5A, the protein level of cytochrome P450 2E1 (CYP2E1) was significantly suppressed by RLTS with a dose-dependent manner. Our findings showed that RLTS was able to counter the CCl4-induced oxidative stress and suppressed oxidative liver injury. As shown in Figure 5B–F, in model group, CCl4-induced liver fibrosis provoked a significant promotion of liver MDA content and obvious reductions of liver GSH-Px, GSH, CAT and SOD activities compared with normal group. Our results showed that RLTS dramatically decreased liver MDA level and markedly increased the activities of GSH-Px, GSH, CAT and SOD. As shown in Figure 5G–I, compared with control group, the levels of heme oxygenase-1 (HO-1), superoxide dismutase 2 (SOD2) and nuclear factor erythroid 2-related factor 2 (Nrf2) were markedly decreased in model group. Their expressions in RLTS (210 mg/kg) group were markedly up-regulated by 2.47-, 4.69-, 2.60-fold, respectively, compared with the model group.


Protective Effect of the Total Saponins from Rosa laevigata Michx Fruit against Carbon Tetrachloride-Induced Liver Fibrosis in Rats.

Dong D, Yin L, Qi Y, Xu L, Peng J - Nutrients (2015)

Effect of Rosa laevigata Michx fruit (RLTS) on the protein expression of CYP2E1 (A); Effects of RLTS on the levels of MDA, GSH-Px, GSH, CAT and SOD in livers (B–F); Effects of RLTS on the protein expressions of HO-1, SOD2 and Nrf2 (G–I). (I) RLTS control; (II) Normal control; (III) model; (IV) RLTS (70 mg/kg) + CCl4; (V) RLTS (140 mg/kg) + CCl4; (VI) RLTS (210 mg/kg) + CCl4; (VII) silymarin (210 mg/kg) + CCl4. Values are expressed as mean ± SD (n = 4). * p < 0.05; ** p < 0.01 vs. model group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488818&req=5

nutrients-07-04829-f005: Effect of Rosa laevigata Michx fruit (RLTS) on the protein expression of CYP2E1 (A); Effects of RLTS on the levels of MDA, GSH-Px, GSH, CAT and SOD in livers (B–F); Effects of RLTS on the protein expressions of HO-1, SOD2 and Nrf2 (G–I). (I) RLTS control; (II) Normal control; (III) model; (IV) RLTS (70 mg/kg) + CCl4; (V) RLTS (140 mg/kg) + CCl4; (VI) RLTS (210 mg/kg) + CCl4; (VII) silymarin (210 mg/kg) + CCl4. Values are expressed as mean ± SD (n = 4). * p < 0.05; ** p < 0.01 vs. model group.
Mentions: As shown in Figure 5A, the protein level of cytochrome P450 2E1 (CYP2E1) was significantly suppressed by RLTS with a dose-dependent manner. Our findings showed that RLTS was able to counter the CCl4-induced oxidative stress and suppressed oxidative liver injury. As shown in Figure 5B–F, in model group, CCl4-induced liver fibrosis provoked a significant promotion of liver MDA content and obvious reductions of liver GSH-Px, GSH, CAT and SOD activities compared with normal group. Our results showed that RLTS dramatically decreased liver MDA level and markedly increased the activities of GSH-Px, GSH, CAT and SOD. As shown in Figure 5G–I, compared with control group, the levels of heme oxygenase-1 (HO-1), superoxide dismutase 2 (SOD2) and nuclear factor erythroid 2-related factor 2 (Nrf2) were markedly decreased in model group. Their expressions in RLTS (210 mg/kg) group were markedly up-regulated by 2.47-, 4.69-, 2.60-fold, respectively, compared with the model group.

Bottom Line: The results showed that RLTS significantly rehabilitated the levels of alanine aminotransferase, aspartate aminotransferase, malondialdehyde, glutathione, glutathione peroxidase, catalase, superoxide dismutase, hydroxyproline, α-smooth muscle actin, collagen I, collagen III and fibronectin, which were confirmed using H&E, Sirius Red and Masson histopathological assays.Further research indicated that RLTS markedly reduced cytochrome P450 2E1 activity, attenuated oxidative stress, and suppressed inflammation.In addition, RLTS facilitated matrix degradation through down-regulation of matrix metalloproteinase2, matrix metalloproteinase 9 and metalloproteinases1, and exerted the anti-fibrotic effects through affecting transforming growth factor β/Smad, focal adhesion kinase/phosphatidylinositol-3-kinase/amino kinase terminal/70-kDa ribosomal S6 Kinase (FAK-PI3K-Akt-p70(S6K)) and mitogen-activated protein kinase (MAPK) signaling pathways.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Dalian Medical University, No. 9 Western Lvshun South Road, Dalian 116044, China. deshidong@163.com.

ABSTRACT
In this study, the protective effect of the total saponins from Rosa laevigata Michx (RLTS) against liver fibrosis induced by carbon tetrachloride (CCl4) in rats was evaluated. The results showed that RLTS significantly rehabilitated the levels of alanine aminotransferase, aspartate aminotransferase, malondialdehyde, glutathione, glutathione peroxidase, catalase, superoxide dismutase, hydroxyproline, α-smooth muscle actin, collagen I, collagen III and fibronectin, which were confirmed using H&E, Sirius Red and Masson histopathological assays. Further research indicated that RLTS markedly reduced cytochrome P450 2E1 activity, attenuated oxidative stress, and suppressed inflammation. In addition, RLTS facilitated matrix degradation through down-regulation of matrix metalloproteinase2, matrix metalloproteinase 9 and metalloproteinases1, and exerted the anti-fibrotic effects through affecting transforming growth factor β/Smad, focal adhesion kinase/phosphatidylinositol-3-kinase/amino kinase terminal/70-kDa ribosomal S6 Kinase (FAK-PI3K-Akt-p70(S6K)) and mitogen-activated protein kinase (MAPK) signaling pathways. Taken together, our data indicate that RLTS can be applied as one effective candidate for the treatment of liver fibrosis in the future.

No MeSH data available.


Related in: MedlinePlus