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Protective Effect of the Total Saponins from Rosa laevigata Michx Fruit against Carbon Tetrachloride-Induced Liver Fibrosis in Rats.

Dong D, Yin L, Qi Y, Xu L, Peng J - Nutrients (2015)

Bottom Line: The results showed that RLTS significantly rehabilitated the levels of alanine aminotransferase, aspartate aminotransferase, malondialdehyde, glutathione, glutathione peroxidase, catalase, superoxide dismutase, hydroxyproline, α-smooth muscle actin, collagen I, collagen III and fibronectin, which were confirmed using H&E, Sirius Red and Masson histopathological assays.Further research indicated that RLTS markedly reduced cytochrome P450 2E1 activity, attenuated oxidative stress, and suppressed inflammation.In addition, RLTS facilitated matrix degradation through down-regulation of matrix metalloproteinase2, matrix metalloproteinase 9 and metalloproteinases1, and exerted the anti-fibrotic effects through affecting transforming growth factor β/Smad, focal adhesion kinase/phosphatidylinositol-3-kinase/amino kinase terminal/70-kDa ribosomal S6 Kinase (FAK-PI3K-Akt-p70(S6K)) and mitogen-activated protein kinase (MAPK) signaling pathways.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Dalian Medical University, No. 9 Western Lvshun South Road, Dalian 116044, China. deshidong@163.com.

ABSTRACT
In this study, the protective effect of the total saponins from Rosa laevigata Michx (RLTS) against liver fibrosis induced by carbon tetrachloride (CCl4) in rats was evaluated. The results showed that RLTS significantly rehabilitated the levels of alanine aminotransferase, aspartate aminotransferase, malondialdehyde, glutathione, glutathione peroxidase, catalase, superoxide dismutase, hydroxyproline, α-smooth muscle actin, collagen I, collagen III and fibronectin, which were confirmed using H&E, Sirius Red and Masson histopathological assays. Further research indicated that RLTS markedly reduced cytochrome P450 2E1 activity, attenuated oxidative stress, and suppressed inflammation. In addition, RLTS facilitated matrix degradation through down-regulation of matrix metalloproteinase2, matrix metalloproteinase 9 and metalloproteinases1, and exerted the anti-fibrotic effects through affecting transforming growth factor β/Smad, focal adhesion kinase/phosphatidylinositol-3-kinase/amino kinase terminal/70-kDa ribosomal S6 Kinase (FAK-PI3K-Akt-p70(S6K)) and mitogen-activated protein kinase (MAPK) signaling pathways. Taken together, our data indicate that RLTS can be applied as one effective candidate for the treatment of liver fibrosis in the future.

No MeSH data available.


Related in: MedlinePlus

Effects of Rosa laevigata Michx fruit (RLTS) on histopathological examination based on H&E staining (A); Effect of RLTS against CCl4-induced liver fibrosis based on transmission electron microscopy (TEM) assay (magnification, 25,000×). The red, blue and pink arrows indicate nuclei, endoplasmic reticulum and mitochondria, respectively (B); Effects of RLTS on serum ALT, AST activities and the bilirubin content (C–E). (I) RLTS control; (II) Normal control; (III) model; (IV) RLTS (70 mg/kg) + CCl4; (V) RLTS (140 mg/kg) + CCl4; (VI) RLTS (210 mg/kg) + CCl4; (VII) silymarin (210 mg/kg) + CCl4. Values are expressed as mean ± SD (n = 10). * p < 0.05; ** p < 0.01 vs. model group.
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nutrients-07-04829-f001: Effects of Rosa laevigata Michx fruit (RLTS) on histopathological examination based on H&E staining (A); Effect of RLTS against CCl4-induced liver fibrosis based on transmission electron microscopy (TEM) assay (magnification, 25,000×). The red, blue and pink arrows indicate nuclei, endoplasmic reticulum and mitochondria, respectively (B); Effects of RLTS on serum ALT, AST activities and the bilirubin content (C–E). (I) RLTS control; (II) Normal control; (III) model; (IV) RLTS (70 mg/kg) + CCl4; (V) RLTS (140 mg/kg) + CCl4; (VI) RLTS (210 mg/kg) + CCl4; (VII) silymarin (210 mg/kg) + CCl4. Values are expressed as mean ± SD (n = 10). * p < 0.05; ** p < 0.01 vs. model group.

Mentions: As shown in Figure 1A, the liver from the model group showed extensive changes including marked necrosis, hepatocyte ballooning and infiltration of inflammatory cells, but the changes were obviously attenuated by RLTS. As shown in Figure 1B, irregular nuclei, dilated endoplasmic reticulum, swollen mitochondria and chromatin condensation were found in model group. However, RLTS treatment significantly attenuated the changes caused by CCl4. As shown in Figure 1C–E, significant increased levels of ALT, AST and bilirubin were observed in model group, which were all markedly reduced by RLTS or silymarin.


Protective Effect of the Total Saponins from Rosa laevigata Michx Fruit against Carbon Tetrachloride-Induced Liver Fibrosis in Rats.

Dong D, Yin L, Qi Y, Xu L, Peng J - Nutrients (2015)

Effects of Rosa laevigata Michx fruit (RLTS) on histopathological examination based on H&E staining (A); Effect of RLTS against CCl4-induced liver fibrosis based on transmission electron microscopy (TEM) assay (magnification, 25,000×). The red, blue and pink arrows indicate nuclei, endoplasmic reticulum and mitochondria, respectively (B); Effects of RLTS on serum ALT, AST activities and the bilirubin content (C–E). (I) RLTS control; (II) Normal control; (III) model; (IV) RLTS (70 mg/kg) + CCl4; (V) RLTS (140 mg/kg) + CCl4; (VI) RLTS (210 mg/kg) + CCl4; (VII) silymarin (210 mg/kg) + CCl4. Values are expressed as mean ± SD (n = 10). * p < 0.05; ** p < 0.01 vs. model group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488818&req=5

nutrients-07-04829-f001: Effects of Rosa laevigata Michx fruit (RLTS) on histopathological examination based on H&E staining (A); Effect of RLTS against CCl4-induced liver fibrosis based on transmission electron microscopy (TEM) assay (magnification, 25,000×). The red, blue and pink arrows indicate nuclei, endoplasmic reticulum and mitochondria, respectively (B); Effects of RLTS on serum ALT, AST activities and the bilirubin content (C–E). (I) RLTS control; (II) Normal control; (III) model; (IV) RLTS (70 mg/kg) + CCl4; (V) RLTS (140 mg/kg) + CCl4; (VI) RLTS (210 mg/kg) + CCl4; (VII) silymarin (210 mg/kg) + CCl4. Values are expressed as mean ± SD (n = 10). * p < 0.05; ** p < 0.01 vs. model group.
Mentions: As shown in Figure 1A, the liver from the model group showed extensive changes including marked necrosis, hepatocyte ballooning and infiltration of inflammatory cells, but the changes were obviously attenuated by RLTS. As shown in Figure 1B, irregular nuclei, dilated endoplasmic reticulum, swollen mitochondria and chromatin condensation were found in model group. However, RLTS treatment significantly attenuated the changes caused by CCl4. As shown in Figure 1C–E, significant increased levels of ALT, AST and bilirubin were observed in model group, which were all markedly reduced by RLTS or silymarin.

Bottom Line: The results showed that RLTS significantly rehabilitated the levels of alanine aminotransferase, aspartate aminotransferase, malondialdehyde, glutathione, glutathione peroxidase, catalase, superoxide dismutase, hydroxyproline, α-smooth muscle actin, collagen I, collagen III and fibronectin, which were confirmed using H&E, Sirius Red and Masson histopathological assays.Further research indicated that RLTS markedly reduced cytochrome P450 2E1 activity, attenuated oxidative stress, and suppressed inflammation.In addition, RLTS facilitated matrix degradation through down-regulation of matrix metalloproteinase2, matrix metalloproteinase 9 and metalloproteinases1, and exerted the anti-fibrotic effects through affecting transforming growth factor β/Smad, focal adhesion kinase/phosphatidylinositol-3-kinase/amino kinase terminal/70-kDa ribosomal S6 Kinase (FAK-PI3K-Akt-p70(S6K)) and mitogen-activated protein kinase (MAPK) signaling pathways.

View Article: PubMed Central - PubMed

Affiliation: College of Pharmacy, Dalian Medical University, No. 9 Western Lvshun South Road, Dalian 116044, China. deshidong@163.com.

ABSTRACT
In this study, the protective effect of the total saponins from Rosa laevigata Michx (RLTS) against liver fibrosis induced by carbon tetrachloride (CCl4) in rats was evaluated. The results showed that RLTS significantly rehabilitated the levels of alanine aminotransferase, aspartate aminotransferase, malondialdehyde, glutathione, glutathione peroxidase, catalase, superoxide dismutase, hydroxyproline, α-smooth muscle actin, collagen I, collagen III and fibronectin, which were confirmed using H&E, Sirius Red and Masson histopathological assays. Further research indicated that RLTS markedly reduced cytochrome P450 2E1 activity, attenuated oxidative stress, and suppressed inflammation. In addition, RLTS facilitated matrix degradation through down-regulation of matrix metalloproteinase2, matrix metalloproteinase 9 and metalloproteinases1, and exerted the anti-fibrotic effects through affecting transforming growth factor β/Smad, focal adhesion kinase/phosphatidylinositol-3-kinase/amino kinase terminal/70-kDa ribosomal S6 Kinase (FAK-PI3K-Akt-p70(S6K)) and mitogen-activated protein kinase (MAPK) signaling pathways. Taken together, our data indicate that RLTS can be applied as one effective candidate for the treatment of liver fibrosis in the future.

No MeSH data available.


Related in: MedlinePlus