Limits...
Pyrosequencing analysis reveals changes in intestinal microbiota of healthy adults who received a daily dose of immunomodulatory probiotic strains.

Plaza-Díaz J, Fernández-Caballero JÁ, Chueca N, García F, Gómez-Llorente C, Sáez-Lara MJ, Fontana L, Gil Á - Nutrients (2015)

Bottom Line: We have recently described the immunomodulatory effects of three probiotic strains (Lactobacillus rhamnosus CNCM I-4036, Lactobacillus paracasei CNCM I-4034, and Bifidobacterium breve CNCM I-4035).The Shannon indices were significantly increased in these two groups.Our results also revealed a significant increase in the Lactobacillus genus after the intervention with L. rhamnosus CNCM I-4036.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology II, School of Pharmacy, University of Granada, Granada 18071, Spain. jrplaza@ugr.es.

ABSTRACT
The colon microbiota plays a crucial role in human gastrointestinal health. Current attempts to manipulate the colon microbiota composition are aimed at finding remedies for various diseases. We have recently described the immunomodulatory effects of three probiotic strains (Lactobacillus rhamnosus CNCM I-4036, Lactobacillus paracasei CNCM I-4034, and Bifidobacterium breve CNCM I-4035). The goal of the present study was to analyze the compositions of the fecal microbiota of healthy adults who received one of these strains using high-throughput 16S ribosomal RNA gene sequencing. Bacteroides was the most abundant genus in the groups that received L. rhamnosus CNCM I-4036 or L. paracasei CNCM I-4034. The Shannon indices were significantly increased in these two groups. Our results also revealed a significant increase in the Lactobacillus genus after the intervention with L. rhamnosus CNCM I-4036. The initially different colon microbiota became homogeneous in the subjects who received L. rhamnosus CNCM I-4036. While some orders that were initially present disappeared after the administration of L. rhamnosus CNCM I-4036, other orders, such as Sphingobacteriales, Nitrospirales, Desulfobacterales, Thiotrichales, and Synergistetes, were detected after the intervention. In summary, our results show that the intake of these three bacterial strains induced changes in the colon microbiota.

No MeSH data available.


Related in: MedlinePlus

Tree diagram of the different L. rhamnosus CNCM I-4036 intake phases. (A) First washout. (B) Intervention. (C) Second washout. Each tree diagram represents the composition of the intestinal microbiota of five healthy volunteers who received L. rhamnosus CNCM I-4036. Colors represent orders. FW, First washout; I, intervention; and SW, second washout.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4488769&req=5

nutrients-07-03999-f003: Tree diagram of the different L. rhamnosus CNCM I-4036 intake phases. (A) First washout. (B) Intervention. (C) Second washout. Each tree diagram represents the composition of the intestinal microbiota of five healthy volunteers who received L. rhamnosus CNCM I-4036. Colors represent orders. FW, First washout; I, intervention; and SW, second washout.

Mentions: The tree diagram (Figure 3A–C) revealed differences in the three time points. The first washout (Figure 3A) presented a characteristic composition at the order level. The orders Gemmatimonadales, Rhodobacterales, and Chroococcales disappeared after the intervention and also after the second washout. The order Nautilales also vanished after the intervention but was detected again after the second washout (t3).


Pyrosequencing analysis reveals changes in intestinal microbiota of healthy adults who received a daily dose of immunomodulatory probiotic strains.

Plaza-Díaz J, Fernández-Caballero JÁ, Chueca N, García F, Gómez-Llorente C, Sáez-Lara MJ, Fontana L, Gil Á - Nutrients (2015)

Tree diagram of the different L. rhamnosus CNCM I-4036 intake phases. (A) First washout. (B) Intervention. (C) Second washout. Each tree diagram represents the composition of the intestinal microbiota of five healthy volunteers who received L. rhamnosus CNCM I-4036. Colors represent orders. FW, First washout; I, intervention; and SW, second washout.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488769&req=5

nutrients-07-03999-f003: Tree diagram of the different L. rhamnosus CNCM I-4036 intake phases. (A) First washout. (B) Intervention. (C) Second washout. Each tree diagram represents the composition of the intestinal microbiota of five healthy volunteers who received L. rhamnosus CNCM I-4036. Colors represent orders. FW, First washout; I, intervention; and SW, second washout.
Mentions: The tree diagram (Figure 3A–C) revealed differences in the three time points. The first washout (Figure 3A) presented a characteristic composition at the order level. The orders Gemmatimonadales, Rhodobacterales, and Chroococcales disappeared after the intervention and also after the second washout. The order Nautilales also vanished after the intervention but was detected again after the second washout (t3).

Bottom Line: We have recently described the immunomodulatory effects of three probiotic strains (Lactobacillus rhamnosus CNCM I-4036, Lactobacillus paracasei CNCM I-4034, and Bifidobacterium breve CNCM I-4035).The Shannon indices were significantly increased in these two groups.Our results also revealed a significant increase in the Lactobacillus genus after the intervention with L. rhamnosus CNCM I-4036.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology II, School of Pharmacy, University of Granada, Granada 18071, Spain. jrplaza@ugr.es.

ABSTRACT
The colon microbiota plays a crucial role in human gastrointestinal health. Current attempts to manipulate the colon microbiota composition are aimed at finding remedies for various diseases. We have recently described the immunomodulatory effects of three probiotic strains (Lactobacillus rhamnosus CNCM I-4036, Lactobacillus paracasei CNCM I-4034, and Bifidobacterium breve CNCM I-4035). The goal of the present study was to analyze the compositions of the fecal microbiota of healthy adults who received one of these strains using high-throughput 16S ribosomal RNA gene sequencing. Bacteroides was the most abundant genus in the groups that received L. rhamnosus CNCM I-4036 or L. paracasei CNCM I-4034. The Shannon indices were significantly increased in these two groups. Our results also revealed a significant increase in the Lactobacillus genus after the intervention with L. rhamnosus CNCM I-4036. The initially different colon microbiota became homogeneous in the subjects who received L. rhamnosus CNCM I-4036. While some orders that were initially present disappeared after the administration of L. rhamnosus CNCM I-4036, other orders, such as Sphingobacteriales, Nitrospirales, Desulfobacterales, Thiotrichales, and Synergistetes, were detected after the intervention. In summary, our results show that the intake of these three bacterial strains induced changes in the colon microbiota.

No MeSH data available.


Related in: MedlinePlus