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Acyl-CoA N-acyltransferase influences fertility by regulating lipid metabolism and jasmonic acid biogenesis in cotton.

Fu W, Shen Y, Hao J, Wu J, Ke L, Wu C, Huang K, Luo B, Xu M, Cheng X, Zhou X, Sun J, Xing C, Sun Y - Sci Rep (2015)

Bottom Line: C312) resulted in indehiscent anthers that were full of pollen, diminished filaments and stamens, and plant sterility.We found GhACNAT was involved in lipid metabolism and jasmonic acid (JA) biosynthesis.In GhACNAT-silenced plants, the expression levels of genes involved in lipid metabolism and jasmonic acid biosynthesis were significantly changed, the amount of JA in leaves and reproductive organs was significantly decreased compared with the amounts in C312.

View Article: PubMed Central - PubMed

Affiliation: 1] College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, Zhejiang, China [2] College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, 310016, Zhejiang, China.

ABSTRACT
Cotton (Gossypium spp.) is an important economic crop and there is obvious heterosis in cotton, fertility has played an important role in this heterosis. However, the genes that exhibit critical roles in anther development and fertility are not well understood. Here, we report an acyl-CoA N-acyltransferase (EC2.3; GhACNAT) that plays a key role in anther development and fertility. Suppression of GhACNAT by virus-induced gene silencing in transgenic cotton (G. hirsutum L. cv. C312) resulted in indehiscent anthers that were full of pollen, diminished filaments and stamens, and plant sterility. We found GhACNAT was involved in lipid metabolism and jasmonic acid (JA) biosynthesis. The genes differentially expressed in GhACNAT-silenced plants and C312 were mainly involved in catalytic activity and transcription regulator activity in lipid metabolism. In GhACNAT-silenced plants, the expression levels of genes involved in lipid metabolism and jasmonic acid biosynthesis were significantly changed, the amount of JA in leaves and reproductive organs was significantly decreased compared with the amounts in C312. Treatments with exogenous methyl jasmonate rescued anther dehiscence and pollen release in GhACNAT-silenced plants and caused self-fertility. The GhACNAT gene may play an important role in controlling cotton fertility by regulating the pathways of lipid synthesis and JA biogenesis.

No MeSH data available.


Related in: MedlinePlus

Functional classification into different categories of differentially expressed fragments from reproductive organs of pCLCrV-empty and transgenic GhACNAT-silenced plants.
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f5: Functional classification into different categories of differentially expressed fragments from reproductive organs of pCLCrV-empty and transgenic GhACNAT-silenced plants.

Mentions: To study differentially expressed genes related to the alteration of reproductive organs in the transgenic GhACNAT-silenced plants, 3 individual plants of wild type C312 and the transgenic pCLCrV-GhACNAT-silenced lines were selected for differential expression analysis by cDNA-AFLP. The stigmas and stamens of the flowers (on the day of anthesis) were picked and collected for total RNA isolation. The differentially expressed genes were selected, and 450 fragments (100–200 bp) were cloned and sequenced. The function annotations of the differentially expressed genes in the reproductive organs of the control plants and the transgenic GhACNAT-silenced plants were divided into several categories (Fig. 5). The main differentially expressed genes were focused on catalytic activity, translation regulator activity and metabolic processes involved in lipid metabolism. Acyl-CoA and acyl-CoA N-acyltransferase were involved in the lipid metabolic pathways.


Acyl-CoA N-acyltransferase influences fertility by regulating lipid metabolism and jasmonic acid biogenesis in cotton.

Fu W, Shen Y, Hao J, Wu J, Ke L, Wu C, Huang K, Luo B, Xu M, Cheng X, Zhou X, Sun J, Xing C, Sun Y - Sci Rep (2015)

Functional classification into different categories of differentially expressed fragments from reproductive organs of pCLCrV-empty and transgenic GhACNAT-silenced plants.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488762&req=5

f5: Functional classification into different categories of differentially expressed fragments from reproductive organs of pCLCrV-empty and transgenic GhACNAT-silenced plants.
Mentions: To study differentially expressed genes related to the alteration of reproductive organs in the transgenic GhACNAT-silenced plants, 3 individual plants of wild type C312 and the transgenic pCLCrV-GhACNAT-silenced lines were selected for differential expression analysis by cDNA-AFLP. The stigmas and stamens of the flowers (on the day of anthesis) were picked and collected for total RNA isolation. The differentially expressed genes were selected, and 450 fragments (100–200 bp) were cloned and sequenced. The function annotations of the differentially expressed genes in the reproductive organs of the control plants and the transgenic GhACNAT-silenced plants were divided into several categories (Fig. 5). The main differentially expressed genes were focused on catalytic activity, translation regulator activity and metabolic processes involved in lipid metabolism. Acyl-CoA and acyl-CoA N-acyltransferase were involved in the lipid metabolic pathways.

Bottom Line: C312) resulted in indehiscent anthers that were full of pollen, diminished filaments and stamens, and plant sterility.We found GhACNAT was involved in lipid metabolism and jasmonic acid (JA) biosynthesis.In GhACNAT-silenced plants, the expression levels of genes involved in lipid metabolism and jasmonic acid biosynthesis were significantly changed, the amount of JA in leaves and reproductive organs was significantly decreased compared with the amounts in C312.

View Article: PubMed Central - PubMed

Affiliation: 1] College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, Zhejiang, China [2] College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, 310016, Zhejiang, China.

ABSTRACT
Cotton (Gossypium spp.) is an important economic crop and there is obvious heterosis in cotton, fertility has played an important role in this heterosis. However, the genes that exhibit critical roles in anther development and fertility are not well understood. Here, we report an acyl-CoA N-acyltransferase (EC2.3; GhACNAT) that plays a key role in anther development and fertility. Suppression of GhACNAT by virus-induced gene silencing in transgenic cotton (G. hirsutum L. cv. C312) resulted in indehiscent anthers that were full of pollen, diminished filaments and stamens, and plant sterility. We found GhACNAT was involved in lipid metabolism and jasmonic acid (JA) biosynthesis. The genes differentially expressed in GhACNAT-silenced plants and C312 were mainly involved in catalytic activity and transcription regulator activity in lipid metabolism. In GhACNAT-silenced plants, the expression levels of genes involved in lipid metabolism and jasmonic acid biosynthesis were significantly changed, the amount of JA in leaves and reproductive organs was significantly decreased compared with the amounts in C312. Treatments with exogenous methyl jasmonate rescued anther dehiscence and pollen release in GhACNAT-silenced plants and caused self-fertility. The GhACNAT gene may play an important role in controlling cotton fertility by regulating the pathways of lipid synthesis and JA biogenesis.

No MeSH data available.


Related in: MedlinePlus