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Acyl-CoA N-acyltransferase influences fertility by regulating lipid metabolism and jasmonic acid biogenesis in cotton.

Fu W, Shen Y, Hao J, Wu J, Ke L, Wu C, Huang K, Luo B, Xu M, Cheng X, Zhou X, Sun J, Xing C, Sun Y - Sci Rep (2015)

Bottom Line: C312) resulted in indehiscent anthers that were full of pollen, diminished filaments and stamens, and plant sterility.We found GhACNAT was involved in lipid metabolism and jasmonic acid (JA) biosynthesis.In GhACNAT-silenced plants, the expression levels of genes involved in lipid metabolism and jasmonic acid biosynthesis were significantly changed, the amount of JA in leaves and reproductive organs was significantly decreased compared with the amounts in C312.

View Article: PubMed Central - PubMed

Affiliation: 1] College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, Zhejiang, China [2] College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, 310016, Zhejiang, China.

ABSTRACT
Cotton (Gossypium spp.) is an important economic crop and there is obvious heterosis in cotton, fertility has played an important role in this heterosis. However, the genes that exhibit critical roles in anther development and fertility are not well understood. Here, we report an acyl-CoA N-acyltransferase (EC2.3; GhACNAT) that plays a key role in anther development and fertility. Suppression of GhACNAT by virus-induced gene silencing in transgenic cotton (G. hirsutum L. cv. C312) resulted in indehiscent anthers that were full of pollen, diminished filaments and stamens, and plant sterility. We found GhACNAT was involved in lipid metabolism and jasmonic acid (JA) biosynthesis. The genes differentially expressed in GhACNAT-silenced plants and C312 were mainly involved in catalytic activity and transcription regulator activity in lipid metabolism. In GhACNAT-silenced plants, the expression levels of genes involved in lipid metabolism and jasmonic acid biosynthesis were significantly changed, the amount of JA in leaves and reproductive organs was significantly decreased compared with the amounts in C312. Treatments with exogenous methyl jasmonate rescued anther dehiscence and pollen release in GhACNAT-silenced plants and caused self-fertility. The GhACNAT gene may play an important role in controlling cotton fertility by regulating the pathways of lipid synthesis and JA biogenesis.

No MeSH data available.


Related in: MedlinePlus

The GhACNAT gene expression pattern in the cotton variety C312.R: root; S: stem; L: leaves, B: bract, P: petals, St: stigmas and stamens, and fibers of different development stages (0-, 5-, 10-, 15-, 20, 25-DPA) in C312. (DPA: days post anthesis). The values are the means±s.ds. for three biological replicates. The asterisks indicate statistically significant differences between the transgenic and WT plants (*P < 0.05, **P < 0.01, Student’s t-test).
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f2: The GhACNAT gene expression pattern in the cotton variety C312.R: root; S: stem; L: leaves, B: bract, P: petals, St: stigmas and stamens, and fibers of different development stages (0-, 5-, 10-, 15-, 20, 25-DPA) in C312. (DPA: days post anthesis). The values are the means±s.ds. for three biological replicates. The asterisks indicate statistically significant differences between the transgenic and WT plants (*P < 0.05, **P < 0.01, Student’s t-test).

Mentions: The GhACNAT gene was expressed at different levels in the tested tissues and organs, including roots, stems, leaves, bracts, petals, stamens stigmas, and fibers of different development stages (0-, 5-, 10-, 15-, 20-, and 25-DPA (days post anthesis)) in C312 (Fig. 2). The expression levels of the GhACNAT gene in the reproductive organs (bracts, petals, stigmas and stamens) were significantly higher than in the vegetative organs (roots, stems, and leaves) and fibers of different developmental stages. The GhACNAT gene maintained significantly high-level expression in the flower organs, including the sepals, petals, stamens and stigmas.


Acyl-CoA N-acyltransferase influences fertility by regulating lipid metabolism and jasmonic acid biogenesis in cotton.

Fu W, Shen Y, Hao J, Wu J, Ke L, Wu C, Huang K, Luo B, Xu M, Cheng X, Zhou X, Sun J, Xing C, Sun Y - Sci Rep (2015)

The GhACNAT gene expression pattern in the cotton variety C312.R: root; S: stem; L: leaves, B: bract, P: petals, St: stigmas and stamens, and fibers of different development stages (0-, 5-, 10-, 15-, 20, 25-DPA) in C312. (DPA: days post anthesis). The values are the means±s.ds. for three biological replicates. The asterisks indicate statistically significant differences between the transgenic and WT plants (*P < 0.05, **P < 0.01, Student’s t-test).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488762&req=5

f2: The GhACNAT gene expression pattern in the cotton variety C312.R: root; S: stem; L: leaves, B: bract, P: petals, St: stigmas and stamens, and fibers of different development stages (0-, 5-, 10-, 15-, 20, 25-DPA) in C312. (DPA: days post anthesis). The values are the means±s.ds. for three biological replicates. The asterisks indicate statistically significant differences between the transgenic and WT plants (*P < 0.05, **P < 0.01, Student’s t-test).
Mentions: The GhACNAT gene was expressed at different levels in the tested tissues and organs, including roots, stems, leaves, bracts, petals, stamens stigmas, and fibers of different development stages (0-, 5-, 10-, 15-, 20-, and 25-DPA (days post anthesis)) in C312 (Fig. 2). The expression levels of the GhACNAT gene in the reproductive organs (bracts, petals, stigmas and stamens) were significantly higher than in the vegetative organs (roots, stems, and leaves) and fibers of different developmental stages. The GhACNAT gene maintained significantly high-level expression in the flower organs, including the sepals, petals, stamens and stigmas.

Bottom Line: C312) resulted in indehiscent anthers that were full of pollen, diminished filaments and stamens, and plant sterility.We found GhACNAT was involved in lipid metabolism and jasmonic acid (JA) biosynthesis.In GhACNAT-silenced plants, the expression levels of genes involved in lipid metabolism and jasmonic acid biosynthesis were significantly changed, the amount of JA in leaves and reproductive organs was significantly decreased compared with the amounts in C312.

View Article: PubMed Central - PubMed

Affiliation: 1] College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, Zhejiang, China [2] College of Life and Environmental Science, Hangzhou Normal University, Hangzhou, 310016, Zhejiang, China.

ABSTRACT
Cotton (Gossypium spp.) is an important economic crop and there is obvious heterosis in cotton, fertility has played an important role in this heterosis. However, the genes that exhibit critical roles in anther development and fertility are not well understood. Here, we report an acyl-CoA N-acyltransferase (EC2.3; GhACNAT) that plays a key role in anther development and fertility. Suppression of GhACNAT by virus-induced gene silencing in transgenic cotton (G. hirsutum L. cv. C312) resulted in indehiscent anthers that were full of pollen, diminished filaments and stamens, and plant sterility. We found GhACNAT was involved in lipid metabolism and jasmonic acid (JA) biosynthesis. The genes differentially expressed in GhACNAT-silenced plants and C312 were mainly involved in catalytic activity and transcription regulator activity in lipid metabolism. In GhACNAT-silenced plants, the expression levels of genes involved in lipid metabolism and jasmonic acid biosynthesis were significantly changed, the amount of JA in leaves and reproductive organs was significantly decreased compared with the amounts in C312. Treatments with exogenous methyl jasmonate rescued anther dehiscence and pollen release in GhACNAT-silenced plants and caused self-fertility. The GhACNAT gene may play an important role in controlling cotton fertility by regulating the pathways of lipid synthesis and JA biogenesis.

No MeSH data available.


Related in: MedlinePlus