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Carbon Quantum Dots for Zebrafish Fluorescence Imaging.

Kang YF, Li YH, Fang YW, Xu Y, Wei XM, Yin XB - Sci Rep (2015)

Bottom Line: The distribution of C-QDs in zebrafish embryos and larvae were successfully observed from their fluorescence emission. the bio-toxicity of C-QDs was tested with zebrafish as model and C-QDs do not interfere to the development of zebrafish embryo.The absorption, distribution, metabolism and excretion route (ADME) of C-QDs in zebrafish was revealed by their distribution.Our work provides the useful information for the researchers interested in studying with zebrafish as a model and the applications of C-QDs.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin Key laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071, China.

ABSTRACT
Carbon quantum dots (C-QDs) are becoming a desirable alternative to metal-based QDs and dye probes owing to their high biocompatibility, low toxicity, ease of preparation, and unique photophysical properties. Herein, we describe fluorescence bioimaging of zebrafish using C-QDs as probe in terms of the preparation of C-QDs, zebrafish husbandry, embryo harvesting, and introduction of C-QDs into embryos and larvae by soaking and microinjection. The multicolor of C-QDs was validated with their imaging for zebrafish embryo. The distribution of C-QDs in zebrafish embryos and larvae were successfully observed from their fluorescence emission. the bio-toxicity of C-QDs was tested with zebrafish as model and C-QDs do not interfere to the development of zebrafish embryo. All of the results confirmed the high biocompatibility and low toxicity of C-QDs as imaging probe. The absorption, distribution, metabolism and excretion route (ADME) of C-QDs in zebrafish was revealed by their distribution. Our work provides the useful information for the researchers interested in studying with zebrafish as a model and the applications of C-QDs. The operations related zebrafish are suitable for the study of the toxicity, adverse effects, transport, and biocompatibility of nanomaterials as well as for drug screening with zebrafish as model.

No MeSH data available.


Related in: MedlinePlus

Brightfield (upper) and fluorescence (lower) images of (A) whole bodies, (B) head, (C) yolk sac, and (D) tail of zebrafish larvae at 84 hpf after soaking for 10 h in C-QDs solution of different concentrations. Enlarged images showing (B) eye and lens, (C) yolk sac and intestine, as well as (D) vessel in tail, with fluorescent images. A 10× ocular lens was used for (A, B, C, and D). A 4× objective lens was used for (A) and a 10× objective lens was used for (B, C, and D). Scale bars, 1.0 mm for (A) and 500 μm for (B), (C) and (D).
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f5: Brightfield (upper) and fluorescence (lower) images of (A) whole bodies, (B) head, (C) yolk sac, and (D) tail of zebrafish larvae at 84 hpf after soaking for 10 h in C-QDs solution of different concentrations. Enlarged images showing (B) eye and lens, (C) yolk sac and intestine, as well as (D) vessel in tail, with fluorescent images. A 10× ocular lens was used for (A, B, C, and D). A 4× objective lens was used for (A) and a 10× objective lens was used for (B, C, and D). Scale bars, 1.0 mm for (A) and 500 μm for (B), (C) and (D).

Mentions: Zebrafish larva was used as a model to validate the in vivo imaging application and tissue distribution of C-QDs. Figure 5 shows images of whole bodies and the amplified parts of zebrafish larva after soaking in C-QDs solution at different concentrations for 10 h. The zebrafish larvae exposed to the C-QDs became brighter and brighter with increased C-QD concentration in a concentration-dependence mode, illustrating C-QDs were successfully introduced into the larvae. After C-QDs enter into the larvae body through swallowing and skin-absorption5152, they accumulate selectively in the head, yolk sac and the tail, showing the tissue-dependent affinity of C-QDs (Fig. 5). The brightness of the dorsal aorta reveals that C-QDs have entered the circulatory system (Fig. 5D), which is important for C-QDs transport in zebrafish. The eyes were the brightest part of the zebrafish head and its brightness increased with C-QDs concentration, and the lens can be readily distinguished from the eyeball (Fig. 5B). This indicates that C-QDs can enter the eye across the blood–ocular barrier. C-QDs in the yolk sac mainly accumulated in the intestine and indicated that C-QDs entered into the digestive system and can be eliminated from the body (Fig. 5C). Some C-QDs were removed from the zebrafish larva by metabolism, which was confirmed by the bright gut (Fig. 5A). The C-QDs preferentially accumulate at the periphery of the zebrafish (Fig. 5B,D), reveals that skin-absorption is one important route for C-QDs entering into zebrafish. The outline of the zebrafish is therefore illustrated by the fluorescence emitted from C-QDs.


Carbon Quantum Dots for Zebrafish Fluorescence Imaging.

Kang YF, Li YH, Fang YW, Xu Y, Wei XM, Yin XB - Sci Rep (2015)

Brightfield (upper) and fluorescence (lower) images of (A) whole bodies, (B) head, (C) yolk sac, and (D) tail of zebrafish larvae at 84 hpf after soaking for 10 h in C-QDs solution of different concentrations. Enlarged images showing (B) eye and lens, (C) yolk sac and intestine, as well as (D) vessel in tail, with fluorescent images. A 10× ocular lens was used for (A, B, C, and D). A 4× objective lens was used for (A) and a 10× objective lens was used for (B, C, and D). Scale bars, 1.0 mm for (A) and 500 μm for (B), (C) and (D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488761&req=5

f5: Brightfield (upper) and fluorescence (lower) images of (A) whole bodies, (B) head, (C) yolk sac, and (D) tail of zebrafish larvae at 84 hpf after soaking for 10 h in C-QDs solution of different concentrations. Enlarged images showing (B) eye and lens, (C) yolk sac and intestine, as well as (D) vessel in tail, with fluorescent images. A 10× ocular lens was used for (A, B, C, and D). A 4× objective lens was used for (A) and a 10× objective lens was used for (B, C, and D). Scale bars, 1.0 mm for (A) and 500 μm for (B), (C) and (D).
Mentions: Zebrafish larva was used as a model to validate the in vivo imaging application and tissue distribution of C-QDs. Figure 5 shows images of whole bodies and the amplified parts of zebrafish larva after soaking in C-QDs solution at different concentrations for 10 h. The zebrafish larvae exposed to the C-QDs became brighter and brighter with increased C-QD concentration in a concentration-dependence mode, illustrating C-QDs were successfully introduced into the larvae. After C-QDs enter into the larvae body through swallowing and skin-absorption5152, they accumulate selectively in the head, yolk sac and the tail, showing the tissue-dependent affinity of C-QDs (Fig. 5). The brightness of the dorsal aorta reveals that C-QDs have entered the circulatory system (Fig. 5D), which is important for C-QDs transport in zebrafish. The eyes were the brightest part of the zebrafish head and its brightness increased with C-QDs concentration, and the lens can be readily distinguished from the eyeball (Fig. 5B). This indicates that C-QDs can enter the eye across the blood–ocular barrier. C-QDs in the yolk sac mainly accumulated in the intestine and indicated that C-QDs entered into the digestive system and can be eliminated from the body (Fig. 5C). Some C-QDs were removed from the zebrafish larva by metabolism, which was confirmed by the bright gut (Fig. 5A). The C-QDs preferentially accumulate at the periphery of the zebrafish (Fig. 5B,D), reveals that skin-absorption is one important route for C-QDs entering into zebrafish. The outline of the zebrafish is therefore illustrated by the fluorescence emitted from C-QDs.

Bottom Line: The distribution of C-QDs in zebrafish embryos and larvae were successfully observed from their fluorescence emission. the bio-toxicity of C-QDs was tested with zebrafish as model and C-QDs do not interfere to the development of zebrafish embryo.The absorption, distribution, metabolism and excretion route (ADME) of C-QDs in zebrafish was revealed by their distribution.Our work provides the useful information for the researchers interested in studying with zebrafish as a model and the applications of C-QDs.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin Key laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071, China.

ABSTRACT
Carbon quantum dots (C-QDs) are becoming a desirable alternative to metal-based QDs and dye probes owing to their high biocompatibility, low toxicity, ease of preparation, and unique photophysical properties. Herein, we describe fluorescence bioimaging of zebrafish using C-QDs as probe in terms of the preparation of C-QDs, zebrafish husbandry, embryo harvesting, and introduction of C-QDs into embryos and larvae by soaking and microinjection. The multicolor of C-QDs was validated with their imaging for zebrafish embryo. The distribution of C-QDs in zebrafish embryos and larvae were successfully observed from their fluorescence emission. the bio-toxicity of C-QDs was tested with zebrafish as model and C-QDs do not interfere to the development of zebrafish embryo. All of the results confirmed the high biocompatibility and low toxicity of C-QDs as imaging probe. The absorption, distribution, metabolism and excretion route (ADME) of C-QDs in zebrafish was revealed by their distribution. Our work provides the useful information for the researchers interested in studying with zebrafish as a model and the applications of C-QDs. The operations related zebrafish are suitable for the study of the toxicity, adverse effects, transport, and biocompatibility of nanomaterials as well as for drug screening with zebrafish as model.

No MeSH data available.


Related in: MedlinePlus