Limits...
Carbon Quantum Dots for Zebrafish Fluorescence Imaging.

Kang YF, Li YH, Fang YW, Xu Y, Wei XM, Yin XB - Sci Rep (2015)

Bottom Line: The distribution of C-QDs in zebrafish embryos and larvae were successfully observed from their fluorescence emission. the bio-toxicity of C-QDs was tested with zebrafish as model and C-QDs do not interfere to the development of zebrafish embryo.The absorption, distribution, metabolism and excretion route (ADME) of C-QDs in zebrafish was revealed by their distribution.Our work provides the useful information for the researchers interested in studying with zebrafish as a model and the applications of C-QDs.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin Key laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071, China.

ABSTRACT
Carbon quantum dots (C-QDs) are becoming a desirable alternative to metal-based QDs and dye probes owing to their high biocompatibility, low toxicity, ease of preparation, and unique photophysical properties. Herein, we describe fluorescence bioimaging of zebrafish using C-QDs as probe in terms of the preparation of C-QDs, zebrafish husbandry, embryo harvesting, and introduction of C-QDs into embryos and larvae by soaking and microinjection. The multicolor of C-QDs was validated with their imaging for zebrafish embryo. The distribution of C-QDs in zebrafish embryos and larvae were successfully observed from their fluorescence emission. the bio-toxicity of C-QDs was tested with zebrafish as model and C-QDs do not interfere to the development of zebrafish embryo. All of the results confirmed the high biocompatibility and low toxicity of C-QDs as imaging probe. The absorption, distribution, metabolism and excretion route (ADME) of C-QDs in zebrafish was revealed by their distribution. Our work provides the useful information for the researchers interested in studying with zebrafish as a model and the applications of C-QDs. The operations related zebrafish are suitable for the study of the toxicity, adverse effects, transport, and biocompatibility of nanomaterials as well as for drug screening with zebrafish as model.

No MeSH data available.


Related in: MedlinePlus

(A) XPS spectrum of C-QDs. High-resolution spectra XPS of C1s (B), N1s (C) and O1s(D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4488761&req=5

f2: (A) XPS spectrum of C-QDs. High-resolution spectra XPS of C1s (B), N1s (C) and O1s(D).

Mentions: X-ray photoelectron spectroscopy (XPS) analysis shows the composition of as-synthesized C-QDs. C, N, and O were found in the C-QDs from the XPS spectrum as shown in Fig. 2A. The C1s peak was resolved into three components centered at 284.6, 285.8, and 287.4 eV, which represent sp2C-sp2C, N-sp2C and C-O/C = O bonds214748, respectively (Fig. 2B). The high-resolution spectra of N1s (Fig. 2C) revealed the presence of both pyridinic (399.2 eV) and pyrrolic (401.2 eV) N atoms2148, illustrating that the C-QDs were successfully doped with nitrogen atoms. Fig. 2C revealed that the O1s peak can be resolved into two components centered at 530.7 and 531.3 eV, representing the presence of the C = O and C-OH/C-O-C groups4950. The XPS results indicate that the surface of the as-synthesized C-QDs is functionalized by multiple oxygen- and nitrogen-containing groups by the reaction between glucose and ethylenediamine.


Carbon Quantum Dots for Zebrafish Fluorescence Imaging.

Kang YF, Li YH, Fang YW, Xu Y, Wei XM, Yin XB - Sci Rep (2015)

(A) XPS spectrum of C-QDs. High-resolution spectra XPS of C1s (B), N1s (C) and O1s(D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488761&req=5

f2: (A) XPS spectrum of C-QDs. High-resolution spectra XPS of C1s (B), N1s (C) and O1s(D).
Mentions: X-ray photoelectron spectroscopy (XPS) analysis shows the composition of as-synthesized C-QDs. C, N, and O were found in the C-QDs from the XPS spectrum as shown in Fig. 2A. The C1s peak was resolved into three components centered at 284.6, 285.8, and 287.4 eV, which represent sp2C-sp2C, N-sp2C and C-O/C = O bonds214748, respectively (Fig. 2B). The high-resolution spectra of N1s (Fig. 2C) revealed the presence of both pyridinic (399.2 eV) and pyrrolic (401.2 eV) N atoms2148, illustrating that the C-QDs were successfully doped with nitrogen atoms. Fig. 2C revealed that the O1s peak can be resolved into two components centered at 530.7 and 531.3 eV, representing the presence of the C = O and C-OH/C-O-C groups4950. The XPS results indicate that the surface of the as-synthesized C-QDs is functionalized by multiple oxygen- and nitrogen-containing groups by the reaction between glucose and ethylenediamine.

Bottom Line: The distribution of C-QDs in zebrafish embryos and larvae were successfully observed from their fluorescence emission. the bio-toxicity of C-QDs was tested with zebrafish as model and C-QDs do not interfere to the development of zebrafish embryo.The absorption, distribution, metabolism and excretion route (ADME) of C-QDs in zebrafish was revealed by their distribution.Our work provides the useful information for the researchers interested in studying with zebrafish as a model and the applications of C-QDs.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin Key laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071, China.

ABSTRACT
Carbon quantum dots (C-QDs) are becoming a desirable alternative to metal-based QDs and dye probes owing to their high biocompatibility, low toxicity, ease of preparation, and unique photophysical properties. Herein, we describe fluorescence bioimaging of zebrafish using C-QDs as probe in terms of the preparation of C-QDs, zebrafish husbandry, embryo harvesting, and introduction of C-QDs into embryos and larvae by soaking and microinjection. The multicolor of C-QDs was validated with their imaging for zebrafish embryo. The distribution of C-QDs in zebrafish embryos and larvae were successfully observed from their fluorescence emission. the bio-toxicity of C-QDs was tested with zebrafish as model and C-QDs do not interfere to the development of zebrafish embryo. All of the results confirmed the high biocompatibility and low toxicity of C-QDs as imaging probe. The absorption, distribution, metabolism and excretion route (ADME) of C-QDs in zebrafish was revealed by their distribution. Our work provides the useful information for the researchers interested in studying with zebrafish as a model and the applications of C-QDs. The operations related zebrafish are suitable for the study of the toxicity, adverse effects, transport, and biocompatibility of nanomaterials as well as for drug screening with zebrafish as model.

No MeSH data available.


Related in: MedlinePlus