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Exploring salivary microbiota in AIDS patients with different periodontal statuses using 454 GS-FLX Titanium pyrosequencing.

Zhang F, He S, Jin J, Dong G, Wu H - Front Cell Infect Microbiol (2015)

Bottom Line: Extracted DNA was used as template to amplify bacterial 16S rRNA.Patients with necrotic periodontitis had a distinct salivary bacterial profile from those with chronic periodontitis.This is the first study using advanced sequencing techniques focused on hospitalized AIDS patients showing the diversity of their salivary microbiota.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University Chengdu, China.

ABSTRACT
Patients with acquired immunodeficiency syndrome (AIDS) are at high risk of opportunistic infections. Oral manifestations have been associated with the level of immunosuppression, these include periodontal diseases, and understanding the microbial populations in the oral cavity is crucial for clinical management. The aim of this study was to examine the salivary bacterial diversity in patients newly admitted to the AIDS ward of the Public Health Clinical Center (China). Saliva samples were collected from 15 patients with AIDS who were randomly recruited between December 2013 and March 2014. Extracted DNA was used as template to amplify bacterial 16S rRNA. Sequencing of the amplicon library was performed using a 454 GS-FLX Titanium sequencing platform. Reads were optimized and clustered into operational taxonomic units for further analysis. A total of 10 bacterial phyla (106 genera) were detected. Firmicutes, Bacteroidetes, and Proteobacteria were preponderant in the salivary microbiota in AIDS patients. The pathogen, Capnocytophaga sp., and others not considered pathogenic such as Neisseria elongata, Streptococcus mitis, and Mycoplasma salivarium but which may be opportunistic infective agents were detected. Dialister pneumosintes, Eubacterium infirmum, Rothia mucilaginosa, and Treponema parvum were preponderant in AIDS patients with periodontitis. Patients with necrotic periodontitis had a distinct salivary bacterial profile from those with chronic periodontitis. This is the first study using advanced sequencing techniques focused on hospitalized AIDS patients showing the diversity of their salivary microbiota.

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Heatmap of relative abundance at species level of salivary bacterial profile in AIDS patients with different periodontal statuses.
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Figure 3: Heatmap of relative abundance at species level of salivary bacterial profile in AIDS patients with different periodontal statuses.

Mentions: Figures 3, 4 show the analysis results for species diversity among samples analyzed by PCoA. A heatmap was used to demonstrate the profile of salivary microbiota in AIDS patients (Figure 3): 71.4% of all samples in the periodontitis group and one sample from the gingivitis group were clustered in one tree, while all the other samples were clustered in the other tree, which were mainly samples from the periodontal health and gingivitis groups. Salivary microbiota in AIDS patients showed discrepancy between groups with and without periodontitis.


Exploring salivary microbiota in AIDS patients with different periodontal statuses using 454 GS-FLX Titanium pyrosequencing.

Zhang F, He S, Jin J, Dong G, Wu H - Front Cell Infect Microbiol (2015)

Heatmap of relative abundance at species level of salivary bacterial profile in AIDS patients with different periodontal statuses.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488753&req=5

Figure 3: Heatmap of relative abundance at species level of salivary bacterial profile in AIDS patients with different periodontal statuses.
Mentions: Figures 3, 4 show the analysis results for species diversity among samples analyzed by PCoA. A heatmap was used to demonstrate the profile of salivary microbiota in AIDS patients (Figure 3): 71.4% of all samples in the periodontitis group and one sample from the gingivitis group were clustered in one tree, while all the other samples were clustered in the other tree, which were mainly samples from the periodontal health and gingivitis groups. Salivary microbiota in AIDS patients showed discrepancy between groups with and without periodontitis.

Bottom Line: Extracted DNA was used as template to amplify bacterial 16S rRNA.Patients with necrotic periodontitis had a distinct salivary bacterial profile from those with chronic periodontitis.This is the first study using advanced sequencing techniques focused on hospitalized AIDS patients showing the diversity of their salivary microbiota.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University Chengdu, China.

ABSTRACT
Patients with acquired immunodeficiency syndrome (AIDS) are at high risk of opportunistic infections. Oral manifestations have been associated with the level of immunosuppression, these include periodontal diseases, and understanding the microbial populations in the oral cavity is crucial for clinical management. The aim of this study was to examine the salivary bacterial diversity in patients newly admitted to the AIDS ward of the Public Health Clinical Center (China). Saliva samples were collected from 15 patients with AIDS who were randomly recruited between December 2013 and March 2014. Extracted DNA was used as template to amplify bacterial 16S rRNA. Sequencing of the amplicon library was performed using a 454 GS-FLX Titanium sequencing platform. Reads were optimized and clustered into operational taxonomic units for further analysis. A total of 10 bacterial phyla (106 genera) were detected. Firmicutes, Bacteroidetes, and Proteobacteria were preponderant in the salivary microbiota in AIDS patients. The pathogen, Capnocytophaga sp., and others not considered pathogenic such as Neisseria elongata, Streptococcus mitis, and Mycoplasma salivarium but which may be opportunistic infective agents were detected. Dialister pneumosintes, Eubacterium infirmum, Rothia mucilaginosa, and Treponema parvum were preponderant in AIDS patients with periodontitis. Patients with necrotic periodontitis had a distinct salivary bacterial profile from those with chronic periodontitis. This is the first study using advanced sequencing techniques focused on hospitalized AIDS patients showing the diversity of their salivary microbiota.

Show MeSH
Related in: MedlinePlus