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Characterisation of Structural Proteins from Chronic Bee Paralysis Virus (CBPV) Using Mass Spectrometry.

Chevin A, Coutard B, Blanchard P, Dabert-Gay AS, Ribière-Chabert M, Thiéry R - Viruses (2015)

Bottom Line: The genomic organization of CBPV and in silico analyses have suggested that RNA 1 encodes non-structural proteins, while RNA 2 encodes structural proteins, which are probably encoded by ORFs 2 and 3.Several polypeptides corresponding to proteins encoded by ORF 2 and 3 on RNA 2 were detected.Their role in the formation of the viral capsid is discussed.

View Article: PubMed Central - PubMed

Affiliation: ANSES, Sophia-Antipolis Laboratory, Bee Diseases Unit, BP 111, 06902 Sophia Antipolis, France. aurore.chevin@free.fr.

ABSTRACT
Chronic bee paralysis virus (CBPV) is the etiological agent of chronic paralysis, an infectious and contagious disease in adult honeybees. CBPV is a positive single-stranded RNA virus which contains two major viral RNA fragments. RNA 1 (3674 nt) and RNA 2 (2305 nt) encode three and four putative open reading frames (ORFs), respectively. RNA 1 is thought to encode the viral RNA-dependent RNA polymerase (RdRp) since the amino acid sequence derived from ORF 3 shares similarities with the RdRP of families Nodaviridae and Tombusviridae. The genomic organization of CBPV and in silico analyses have suggested that RNA 1 encodes non-structural proteins, while RNA 2 encodes structural proteins, which are probably encoded by ORFs 2 and 3. In this study, purified CBPV particles were used to characterize virion proteins by mass spectrometry. Several polypeptides corresponding to proteins encoded by ORF 2 and 3 on RNA 2 were detected. Their role in the formation of the viral capsid is discussed.

No MeSH data available.


Related in: MedlinePlus

Diagram of the predicted genome organization of CBPV RNA 1 (A) and RNA 2 (B). Seven putative ORFs are indicated with their positions and putative amino acid sequence length. aa: Amino acid; RdRp: RNA-dependent RNA polymerase; pSP: predicted structural protein; hSP: hypothetical structural protein.
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viruses-07-02774-f001: Diagram of the predicted genome organization of CBPV RNA 1 (A) and RNA 2 (B). Seven putative ORFs are indicated with their positions and putative amino acid sequence length. aa: Amino acid; RdRp: RNA-dependent RNA polymerase; pSP: predicted structural protein; hSP: hypothetical structural protein.

Mentions: Chronic paralysis is an infectious and contagious disease of adult honeybees (Apis mellifera L.). It is known to induce a cluster of clinical signs, such as trembling and flightless bees crawling at the hive entrance [1]. The viral agent that causes this disease is the chronic bee paralysis virus (CBPV). CBPV shows neurotropism in concomitance with observed clinical signs [2]. The viral particle is anisometric and non-enveloped, its size being about 30–60 nm in length and 20 nm in width [3]. CBPV is a positive single-stranded fragmented RNA virus; its genome is composed of two major RNA fragments that have been completely sequenced, RNA 1 (3674 nt) and RNA 2 (2305 nt) [4]. Bioinformatic analysis of RNA sequences has shown that there are seven putative overlapping open reading frames (ORFs), three on RNA 1 and four on RNA 2 [4] (Figure 1).


Characterisation of Structural Proteins from Chronic Bee Paralysis Virus (CBPV) Using Mass Spectrometry.

Chevin A, Coutard B, Blanchard P, Dabert-Gay AS, Ribière-Chabert M, Thiéry R - Viruses (2015)

Diagram of the predicted genome organization of CBPV RNA 1 (A) and RNA 2 (B). Seven putative ORFs are indicated with their positions and putative amino acid sequence length. aa: Amino acid; RdRp: RNA-dependent RNA polymerase; pSP: predicted structural protein; hSP: hypothetical structural protein.
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Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4488741&req=5

viruses-07-02774-f001: Diagram of the predicted genome organization of CBPV RNA 1 (A) and RNA 2 (B). Seven putative ORFs are indicated with their positions and putative amino acid sequence length. aa: Amino acid; RdRp: RNA-dependent RNA polymerase; pSP: predicted structural protein; hSP: hypothetical structural protein.
Mentions: Chronic paralysis is an infectious and contagious disease of adult honeybees (Apis mellifera L.). It is known to induce a cluster of clinical signs, such as trembling and flightless bees crawling at the hive entrance [1]. The viral agent that causes this disease is the chronic bee paralysis virus (CBPV). CBPV shows neurotropism in concomitance with observed clinical signs [2]. The viral particle is anisometric and non-enveloped, its size being about 30–60 nm in length and 20 nm in width [3]. CBPV is a positive single-stranded fragmented RNA virus; its genome is composed of two major RNA fragments that have been completely sequenced, RNA 1 (3674 nt) and RNA 2 (2305 nt) [4]. Bioinformatic analysis of RNA sequences has shown that there are seven putative overlapping open reading frames (ORFs), three on RNA 1 and four on RNA 2 [4] (Figure 1).

Bottom Line: The genomic organization of CBPV and in silico analyses have suggested that RNA 1 encodes non-structural proteins, while RNA 2 encodes structural proteins, which are probably encoded by ORFs 2 and 3.Several polypeptides corresponding to proteins encoded by ORF 2 and 3 on RNA 2 were detected.Their role in the formation of the viral capsid is discussed.

View Article: PubMed Central - PubMed

Affiliation: ANSES, Sophia-Antipolis Laboratory, Bee Diseases Unit, BP 111, 06902 Sophia Antipolis, France. aurore.chevin@free.fr.

ABSTRACT
Chronic bee paralysis virus (CBPV) is the etiological agent of chronic paralysis, an infectious and contagious disease in adult honeybees. CBPV is a positive single-stranded RNA virus which contains two major viral RNA fragments. RNA 1 (3674 nt) and RNA 2 (2305 nt) encode three and four putative open reading frames (ORFs), respectively. RNA 1 is thought to encode the viral RNA-dependent RNA polymerase (RdRp) since the amino acid sequence derived from ORF 3 shares similarities with the RdRP of families Nodaviridae and Tombusviridae. The genomic organization of CBPV and in silico analyses have suggested that RNA 1 encodes non-structural proteins, while RNA 2 encodes structural proteins, which are probably encoded by ORFs 2 and 3. In this study, purified CBPV particles were used to characterize virion proteins by mass spectrometry. Several polypeptides corresponding to proteins encoded by ORF 2 and 3 on RNA 2 were detected. Their role in the formation of the viral capsid is discussed.

No MeSH data available.


Related in: MedlinePlus