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Effects of Wheat Naturally Contaminated with Fusarium Mycotoxins on Growth Performance and Selected Health Indices of Red Tilapia (Oreochromis niloticus × O. mossambicus).

Tola S, Bureau DP, Hooft JM, Beamish FW, Sulyok M, Krska R, Encarnação P, Petkam R - Toxins (Basel) (2015)

Bottom Line: Groups of 50 fish were randomly allocated into each of 20 aquaria and fed to near-satiety for eight weeks.Growth rate, feed intake and feed efficiency of fish fed the experimental diets decreased linearly with increasing levels of Fusarium mycotoxins (p < 0.05).Though there was no clear evidence of overt DON toxicity to red tilapia, it is recommended that feed ingredients should be screened for Fusarium mycotoxin contamination to ensure optimal growth performance.

View Article: PubMed Central - PubMed

Affiliation: Department of Fisheries, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand. pattola4445@gmail.com.

ABSTRACT
An 8-week feeding trial was conducted to examine effects of wheat naturally contaminated with Fusarium mycotoxins (deoxynivalenol, DON 41 mg·kg(-1)) on growth performance and selected health indices of red tilapia (Oreochromis niloticus × O. mossambicus; initial weight = 4.3 g/fish). Five experimental diets were formulated by replacement of clean wheat with naturally contaminated wheat resulting in graded levels of DON and zearalenone (ZEN) (Diet 1 0.07/0.01, Diet 2 0.31/0.09, Diet 3 0.50/0.21, Diet 4 0.92/0.37 and Diet 5 1.15/0.98 mg·kg(-1)). Groups of 50 fish were randomly allocated into each of 20 aquaria and fed to near-satiety for eight weeks. Growth rate, feed intake and feed efficiency of fish fed the experimental diets decreased linearly with increasing levels of Fusarium mycotoxins (p < 0.05). Although growth depression was associated with feeding diets naturally contaminated with Fusarium mycotoxins, especially DON, no biochemical and histopathological parameters measured in blood and liver appeared affected by Fusarium mycotoxin concentrations of diets (p > 0.05). Though there was no clear evidence of overt DON toxicity to red tilapia, it is recommended that feed ingredients should be screened for Fusarium mycotoxin contamination to ensure optimal growth performance.

No MeSH data available.


Related in: MedlinePlus

Light microscopy (×250, H & E stain) of the liver of red tilapia fed experimental diets for eight weeks. (a) Liver of red tilapia fed control diet (0.07 mg DON kg−1); (b) distribution of focal necrosis (arrow) in liver of red tilapia fed Diet 3 (0.50 mg DON kg−1); (c) cytoplasmic vacuolation in liver of red tilapia fed Diet 2 (0.31 mg DON kg−1) and (d) subcapsular edema (arrow) in liver of red tilapia fed Diet 2 (0.31 mg DON kg−1).
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toxins-07-01929-f001: Light microscopy (×250, H & E stain) of the liver of red tilapia fed experimental diets for eight weeks. (a) Liver of red tilapia fed control diet (0.07 mg DON kg−1); (b) distribution of focal necrosis (arrow) in liver of red tilapia fed Diet 3 (0.50 mg DON kg−1); (c) cytoplasmic vacuolation in liver of red tilapia fed Diet 2 (0.31 mg DON kg−1) and (d) subcapsular edema (arrow) in liver of red tilapia fed Diet 2 (0.31 mg DON kg−1).

Mentions: Although histopathological change is not a toxicity-specific response, hepatocytes are known to respond to a variety of toxicants [54]. Most red tilapia fed the control and other diets had healthy livers (n = 12), only few livers appeared to have changes upon gross and microscopic examinations (Figure 1 (a)). Areas of focal necrosis were observed in red tilapia fed diet 3 (Figure 1 (b)) and 5. Cytoplasmic vacuolation was observed in each liver from fish fed diet 2 (Figure 1 (c)) and 3. Seven samples of fish fed diets 2 (Figure 1 (d)), 3 and 4 showed subcapsular edema in both of the gross and microscopic examinations.


Effects of Wheat Naturally Contaminated with Fusarium Mycotoxins on Growth Performance and Selected Health Indices of Red Tilapia (Oreochromis niloticus × O. mossambicus).

Tola S, Bureau DP, Hooft JM, Beamish FW, Sulyok M, Krska R, Encarnação P, Petkam R - Toxins (Basel) (2015)

Light microscopy (×250, H & E stain) of the liver of red tilapia fed experimental diets for eight weeks. (a) Liver of red tilapia fed control diet (0.07 mg DON kg−1); (b) distribution of focal necrosis (arrow) in liver of red tilapia fed Diet 3 (0.50 mg DON kg−1); (c) cytoplasmic vacuolation in liver of red tilapia fed Diet 2 (0.31 mg DON kg−1) and (d) subcapsular edema (arrow) in liver of red tilapia fed Diet 2 (0.31 mg DON kg−1).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488682&req=5

toxins-07-01929-f001: Light microscopy (×250, H & E stain) of the liver of red tilapia fed experimental diets for eight weeks. (a) Liver of red tilapia fed control diet (0.07 mg DON kg−1); (b) distribution of focal necrosis (arrow) in liver of red tilapia fed Diet 3 (0.50 mg DON kg−1); (c) cytoplasmic vacuolation in liver of red tilapia fed Diet 2 (0.31 mg DON kg−1) and (d) subcapsular edema (arrow) in liver of red tilapia fed Diet 2 (0.31 mg DON kg−1).
Mentions: Although histopathological change is not a toxicity-specific response, hepatocytes are known to respond to a variety of toxicants [54]. Most red tilapia fed the control and other diets had healthy livers (n = 12), only few livers appeared to have changes upon gross and microscopic examinations (Figure 1 (a)). Areas of focal necrosis were observed in red tilapia fed diet 3 (Figure 1 (b)) and 5. Cytoplasmic vacuolation was observed in each liver from fish fed diet 2 (Figure 1 (c)) and 3. Seven samples of fish fed diets 2 (Figure 1 (d)), 3 and 4 showed subcapsular edema in both of the gross and microscopic examinations.

Bottom Line: Groups of 50 fish were randomly allocated into each of 20 aquaria and fed to near-satiety for eight weeks.Growth rate, feed intake and feed efficiency of fish fed the experimental diets decreased linearly with increasing levels of Fusarium mycotoxins (p < 0.05).Though there was no clear evidence of overt DON toxicity to red tilapia, it is recommended that feed ingredients should be screened for Fusarium mycotoxin contamination to ensure optimal growth performance.

View Article: PubMed Central - PubMed

Affiliation: Department of Fisheries, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand. pattola4445@gmail.com.

ABSTRACT
An 8-week feeding trial was conducted to examine effects of wheat naturally contaminated with Fusarium mycotoxins (deoxynivalenol, DON 41 mg·kg(-1)) on growth performance and selected health indices of red tilapia (Oreochromis niloticus × O. mossambicus; initial weight = 4.3 g/fish). Five experimental diets were formulated by replacement of clean wheat with naturally contaminated wheat resulting in graded levels of DON and zearalenone (ZEN) (Diet 1 0.07/0.01, Diet 2 0.31/0.09, Diet 3 0.50/0.21, Diet 4 0.92/0.37 and Diet 5 1.15/0.98 mg·kg(-1)). Groups of 50 fish were randomly allocated into each of 20 aquaria and fed to near-satiety for eight weeks. Growth rate, feed intake and feed efficiency of fish fed the experimental diets decreased linearly with increasing levels of Fusarium mycotoxins (p < 0.05). Although growth depression was associated with feeding diets naturally contaminated with Fusarium mycotoxins, especially DON, no biochemical and histopathological parameters measured in blood and liver appeared affected by Fusarium mycotoxin concentrations of diets (p > 0.05). Though there was no clear evidence of overt DON toxicity to red tilapia, it is recommended that feed ingredients should be screened for Fusarium mycotoxin contamination to ensure optimal growth performance.

No MeSH data available.


Related in: MedlinePlus