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Anti-fibrotic effect of natural toxin bee venom on animal model of unilateral ureteral obstruction.

An HJ, Kim KH, Lee WR, Kim JY, Lee SJ, Pak SC, Han SM, Park KK - Toxins (Basel) (2015)

Bottom Line: However, BV treatment markedly reduced these reactions compared with untreated UUO mice.The expression levels of TNF-α and IL-1β were significantly reduced in BV treated mice compared with UUO mice.In conclusion, BV may be a useful therapeutic agent for the prevention of fibrosis that characterizes progression of chronic kidney disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, College of Medicine, Catholic University of Daegu, 3056-6, Daemyung-4-Dong, Nam-gu, Daegu 705-718, Korea. ahj119@cu.ac.kr.

ABSTRACT
Progressive renal fibrosis is the final common pathway for all kidney diseases leading to chronic renal failure. Bee venom (BV) has been widely used as a traditional medicine for various diseases. However, the precise mechanism of BV in ameliorating the renal fibrosis is not fully understood. To investigate the therapeutic effects of BV against unilateral ureteral obstruction (UUO)-induced renal fibrosis, BV was given intraperitoneally after ureteral ligation. At seven days after UUO surgery, the kidney tissues were collected for protein analysis and histologic examination. Histological observation revealed that UUO induced a considerable increase in the number of infiltrated inflammatory cells. However, BV treatment markedly reduced these reactions compared with untreated UUO mice. The expression levels of TNF-α and IL-1β were significantly reduced in BV treated mice compared with UUO mice. In addition, treatment with BV significantly inhibited TGF-β1 and fibronectin expression in UUO mice. Moreover, the expression of α-SMA was markedly withdrawn after treatment with BV. These findings suggest that BV attenuates renal fibrosis and reduces inflammatory responses by suppression of multiple growth factor-mediated pro-fibrotic genes. In conclusion, BV may be a useful therapeutic agent for the prevention of fibrosis that characterizes progression of chronic kidney disease.

No MeSH data available.


Related in: MedlinePlus

BV abolishes the expression of α-SMA in obstructed kidneys. (A) Immunofluorescence staining shows that BV treatment reduces α-SMA positive cells in the kidneys at seven days after UUO surgery. Visible green color indicates α-SMA. Representative images from each study group. (B) Immunofluorescence staining was used to evaluate the extent of α-SMA, which was subsequently quantified. Magnification 200×. Results are expressed as means ± SE of three independent determinations. *p < 0.05 vs. NC group. †p < 0.05 vs. UUO group.
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toxins-07-01917-f004: BV abolishes the expression of α-SMA in obstructed kidneys. (A) Immunofluorescence staining shows that BV treatment reduces α-SMA positive cells in the kidneys at seven days after UUO surgery. Visible green color indicates α-SMA. Representative images from each study group. (B) Immunofluorescence staining was used to evaluate the extent of α-SMA, which was subsequently quantified. Magnification 200×. Results are expressed as means ± SE of three independent determinations. *p < 0.05 vs. NC group. †p < 0.05 vs. UUO group.

Mentions: To investigate the ability of BV to suppress myofibroblast activation, this study examined the expression of α-SMA, a representative marker of activated myofibroblasts, by immunofluorescence staining. In normal kidneys, α-SMA positive cells are found only in the blood vessel wall. However, α-SMA positive cells are scattered in the interstitial space of obstructed kidneys, whereas this population of cells was reduced significantly by BV treatment (Figure 4). This data shows clearly that BV plays a critical role in the inactivation of renal fibroblasts after obstructive injury.


Anti-fibrotic effect of natural toxin bee venom on animal model of unilateral ureteral obstruction.

An HJ, Kim KH, Lee WR, Kim JY, Lee SJ, Pak SC, Han SM, Park KK - Toxins (Basel) (2015)

BV abolishes the expression of α-SMA in obstructed kidneys. (A) Immunofluorescence staining shows that BV treatment reduces α-SMA positive cells in the kidneys at seven days after UUO surgery. Visible green color indicates α-SMA. Representative images from each study group. (B) Immunofluorescence staining was used to evaluate the extent of α-SMA, which was subsequently quantified. Magnification 200×. Results are expressed as means ± SE of three independent determinations. *p < 0.05 vs. NC group. †p < 0.05 vs. UUO group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488681&req=5

toxins-07-01917-f004: BV abolishes the expression of α-SMA in obstructed kidneys. (A) Immunofluorescence staining shows that BV treatment reduces α-SMA positive cells in the kidneys at seven days after UUO surgery. Visible green color indicates α-SMA. Representative images from each study group. (B) Immunofluorescence staining was used to evaluate the extent of α-SMA, which was subsequently quantified. Magnification 200×. Results are expressed as means ± SE of three independent determinations. *p < 0.05 vs. NC group. †p < 0.05 vs. UUO group.
Mentions: To investigate the ability of BV to suppress myofibroblast activation, this study examined the expression of α-SMA, a representative marker of activated myofibroblasts, by immunofluorescence staining. In normal kidneys, α-SMA positive cells are found only in the blood vessel wall. However, α-SMA positive cells are scattered in the interstitial space of obstructed kidneys, whereas this population of cells was reduced significantly by BV treatment (Figure 4). This data shows clearly that BV plays a critical role in the inactivation of renal fibroblasts after obstructive injury.

Bottom Line: However, BV treatment markedly reduced these reactions compared with untreated UUO mice.The expression levels of TNF-α and IL-1β were significantly reduced in BV treated mice compared with UUO mice.In conclusion, BV may be a useful therapeutic agent for the prevention of fibrosis that characterizes progression of chronic kidney disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, College of Medicine, Catholic University of Daegu, 3056-6, Daemyung-4-Dong, Nam-gu, Daegu 705-718, Korea. ahj119@cu.ac.kr.

ABSTRACT
Progressive renal fibrosis is the final common pathway for all kidney diseases leading to chronic renal failure. Bee venom (BV) has been widely used as a traditional medicine for various diseases. However, the precise mechanism of BV in ameliorating the renal fibrosis is not fully understood. To investigate the therapeutic effects of BV against unilateral ureteral obstruction (UUO)-induced renal fibrosis, BV was given intraperitoneally after ureteral ligation. At seven days after UUO surgery, the kidney tissues were collected for protein analysis and histologic examination. Histological observation revealed that UUO induced a considerable increase in the number of infiltrated inflammatory cells. However, BV treatment markedly reduced these reactions compared with untreated UUO mice. The expression levels of TNF-α and IL-1β were significantly reduced in BV treated mice compared with UUO mice. In addition, treatment with BV significantly inhibited TGF-β1 and fibronectin expression in UUO mice. Moreover, the expression of α-SMA was markedly withdrawn after treatment with BV. These findings suggest that BV attenuates renal fibrosis and reduces inflammatory responses by suppression of multiple growth factor-mediated pro-fibrotic genes. In conclusion, BV may be a useful therapeutic agent for the prevention of fibrosis that characterizes progression of chronic kidney disease.

No MeSH data available.


Related in: MedlinePlus