Limits...
Anti-fibrotic effect of natural toxin bee venom on animal model of unilateral ureteral obstruction.

An HJ, Kim KH, Lee WR, Kim JY, Lee SJ, Pak SC, Han SM, Park KK - Toxins (Basel) (2015)

Bottom Line: However, BV treatment markedly reduced these reactions compared with untreated UUO mice.The expression levels of TNF-α and IL-1β were significantly reduced in BV treated mice compared with UUO mice.In conclusion, BV may be a useful therapeutic agent for the prevention of fibrosis that characterizes progression of chronic kidney disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, College of Medicine, Catholic University of Daegu, 3056-6, Daemyung-4-Dong, Nam-gu, Daegu 705-718, Korea. ahj119@cu.ac.kr.

ABSTRACT
Progressive renal fibrosis is the final common pathway for all kidney diseases leading to chronic renal failure. Bee venom (BV) has been widely used as a traditional medicine for various diseases. However, the precise mechanism of BV in ameliorating the renal fibrosis is not fully understood. To investigate the therapeutic effects of BV against unilateral ureteral obstruction (UUO)-induced renal fibrosis, BV was given intraperitoneally after ureteral ligation. At seven days after UUO surgery, the kidney tissues were collected for protein analysis and histologic examination. Histological observation revealed that UUO induced a considerable increase in the number of infiltrated inflammatory cells. However, BV treatment markedly reduced these reactions compared with untreated UUO mice. The expression levels of TNF-α and IL-1β were significantly reduced in BV treated mice compared with UUO mice. In addition, treatment with BV significantly inhibited TGF-β1 and fibronectin expression in UUO mice. Moreover, the expression of α-SMA was markedly withdrawn after treatment with BV. These findings suggest that BV attenuates renal fibrosis and reduces inflammatory responses by suppression of multiple growth factor-mediated pro-fibrotic genes. In conclusion, BV may be a useful therapeutic agent for the prevention of fibrosis that characterizes progression of chronic kidney disease.

No MeSH data available.


Related in: MedlinePlus

BV inhibits renal fibrosis in obstructed kidney. (A) Histological sections of mouse kidney stained with H&E at seven days after UUO surgery. (B) Kidney sections are stained with Masson’s trichrome, which accentuates interstitial fibrosis by staining collagen blue. (C) Masson’s trichrome staining was used to evaluate the extent of renal fibrosis which was subsequently quantified. NC, normal control; UUO, kidney injury induced by UUO; UUO+BV, UUO treated with 0.01 mg/kg of BV. Representative images from each study group. Magnification 400×. Results are expressed as means ± SE of three independent determinations. *p < 0.05 vs. NC group. †p < 0.05 vs. UUO group.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4488681&req=5

toxins-07-01917-f001: BV inhibits renal fibrosis in obstructed kidney. (A) Histological sections of mouse kidney stained with H&E at seven days after UUO surgery. (B) Kidney sections are stained with Masson’s trichrome, which accentuates interstitial fibrosis by staining collagen blue. (C) Masson’s trichrome staining was used to evaluate the extent of renal fibrosis which was subsequently quantified. NC, normal control; UUO, kidney injury induced by UUO; UUO+BV, UUO treated with 0.01 mg/kg of BV. Representative images from each study group. Magnification 400×. Results are expressed as means ± SE of three independent determinations. *p < 0.05 vs. NC group. †p < 0.05 vs. UUO group.

Mentions: The morphological changes in the kidney tissue caused by UUO were visualized in sections stained by hematoxylin and eosin (Figure 1A). Tubular dilatation with flattening of epithelial cells was visualized in UUO kidneys. However, BV treatment significantly reduced these changes when compared to the UUO group. BV attenuated renal histologic damage in UUO mice. The extent of collagen deposition was viewed using Masson’s trichrome staining of renal tissue (Figure 1B) and renal fibrosis was calculated using a well described semiquantitative score derived from the percentage of the positive staining per grid field (Figure 1C). Seven days after UUO surgery, the UUO group demonstrated significant interstitial fibrosis compared with the NC group. However, there was a significant reduction in the number of collagen fibers in the BV treated mice.


Anti-fibrotic effect of natural toxin bee venom on animal model of unilateral ureteral obstruction.

An HJ, Kim KH, Lee WR, Kim JY, Lee SJ, Pak SC, Han SM, Park KK - Toxins (Basel) (2015)

BV inhibits renal fibrosis in obstructed kidney. (A) Histological sections of mouse kidney stained with H&E at seven days after UUO surgery. (B) Kidney sections are stained with Masson’s trichrome, which accentuates interstitial fibrosis by staining collagen blue. (C) Masson’s trichrome staining was used to evaluate the extent of renal fibrosis which was subsequently quantified. NC, normal control; UUO, kidney injury induced by UUO; UUO+BV, UUO treated with 0.01 mg/kg of BV. Representative images from each study group. Magnification 400×. Results are expressed as means ± SE of three independent determinations. *p < 0.05 vs. NC group. †p < 0.05 vs. UUO group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488681&req=5

toxins-07-01917-f001: BV inhibits renal fibrosis in obstructed kidney. (A) Histological sections of mouse kidney stained with H&E at seven days after UUO surgery. (B) Kidney sections are stained with Masson’s trichrome, which accentuates interstitial fibrosis by staining collagen blue. (C) Masson’s trichrome staining was used to evaluate the extent of renal fibrosis which was subsequently quantified. NC, normal control; UUO, kidney injury induced by UUO; UUO+BV, UUO treated with 0.01 mg/kg of BV. Representative images from each study group. Magnification 400×. Results are expressed as means ± SE of three independent determinations. *p < 0.05 vs. NC group. †p < 0.05 vs. UUO group.
Mentions: The morphological changes in the kidney tissue caused by UUO were visualized in sections stained by hematoxylin and eosin (Figure 1A). Tubular dilatation with flattening of epithelial cells was visualized in UUO kidneys. However, BV treatment significantly reduced these changes when compared to the UUO group. BV attenuated renal histologic damage in UUO mice. The extent of collagen deposition was viewed using Masson’s trichrome staining of renal tissue (Figure 1B) and renal fibrosis was calculated using a well described semiquantitative score derived from the percentage of the positive staining per grid field (Figure 1C). Seven days after UUO surgery, the UUO group demonstrated significant interstitial fibrosis compared with the NC group. However, there was a significant reduction in the number of collagen fibers in the BV treated mice.

Bottom Line: However, BV treatment markedly reduced these reactions compared with untreated UUO mice.The expression levels of TNF-α and IL-1β were significantly reduced in BV treated mice compared with UUO mice.In conclusion, BV may be a useful therapeutic agent for the prevention of fibrosis that characterizes progression of chronic kidney disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, College of Medicine, Catholic University of Daegu, 3056-6, Daemyung-4-Dong, Nam-gu, Daegu 705-718, Korea. ahj119@cu.ac.kr.

ABSTRACT
Progressive renal fibrosis is the final common pathway for all kidney diseases leading to chronic renal failure. Bee venom (BV) has been widely used as a traditional medicine for various diseases. However, the precise mechanism of BV in ameliorating the renal fibrosis is not fully understood. To investigate the therapeutic effects of BV against unilateral ureteral obstruction (UUO)-induced renal fibrosis, BV was given intraperitoneally after ureteral ligation. At seven days after UUO surgery, the kidney tissues were collected for protein analysis and histologic examination. Histological observation revealed that UUO induced a considerable increase in the number of infiltrated inflammatory cells. However, BV treatment markedly reduced these reactions compared with untreated UUO mice. The expression levels of TNF-α and IL-1β were significantly reduced in BV treated mice compared with UUO mice. In addition, treatment with BV significantly inhibited TGF-β1 and fibronectin expression in UUO mice. Moreover, the expression of α-SMA was markedly withdrawn after treatment with BV. These findings suggest that BV attenuates renal fibrosis and reduces inflammatory responses by suppression of multiple growth factor-mediated pro-fibrotic genes. In conclusion, BV may be a useful therapeutic agent for the prevention of fibrosis that characterizes progression of chronic kidney disease.

No MeSH data available.


Related in: MedlinePlus