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Development of myenteric plexus in human foetuses: a quantitative study.

Singh S, Shariff A, Roy T, Das T, Rani N - Anat Cell Biol (2015)

Bottom Line: This study revealed that the maturational event increases after 17WG and extensive innervations is established at 23WG.During prenatal life there is an increase in the neuronal cell size from 14-23WG signifying maturational process.Such studies are essential for clinicians and surgeons to correlate the normal and pathologic development of the enteric nervous system.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, All India Institute of Medical Sciences, New Delhi, India.

ABSTRACT
Maturation of neurons of the myenteric plexus (MP) of human fetal sigmoid colon was studied at various weeks of gestation (WG). There is abundant literature on the development of MP in various segments of the gut but there are fewer reports on the development of MP in human sigmoid colon which is a site of various disorders. Sigmoid colonic segments from 12 aborted foetuses aged 14-23WG were processed for NADPH histochemistry. Stereologic evaluation of the neuronal cell profiles, numerical density, number of neurons per ganglion and myenteric fraction was conducted using using imageJ software. According to gestational age, foetuses were assigned into two groups (group 1 [n=7], less than <17WG and group 2 [n=5], more than >17WG). The overall size of neuronal cell profiles in the MP was significantly increased (P<0.05). The numerical density of neurons decreased in group 2 in comparison to group 1, the number of neurons per ganglion and myenteric fraction were increased in group 2 but all these were not statistically significant. This study revealed that the maturational event increases after 17WG and extensive innervations is established at 23WG. During prenatal life there is an increase in the neuronal cell size from 14-23WG signifying maturational process. Such studies are essential for clinicians and surgeons to correlate the normal and pathologic development of the enteric nervous system.

No MeSH data available.


Related in: MedlinePlus

Microphotograph of cross-section of human foetal sigmoid colon in NADPH-diaphorase stain at 14 weeks of gestation (WG) and 16WG. (A) Myenteric plexus (MP) at 14WG having neurons (N) with round to oval nucleus (n) and scanty cytoplasm. Neuronal processes (NP) can be clearly demarcated. Scattered neurons and thin nerve fibres present in submucosa (SM). (B) There is progressive increase in the sizes of myenteric ganglia (MG) and N at 16WG. Nerve fibres increase in inner circular muscle (CM). There is also gradually increase in neuropil. Submucosal plexus (SP) present in the SM towards the CM. LM, outer longitudinal muscle. Scale bar=50 µm (A, B). Reproduced from J Morphol Sci 2013;30:156-66 [18], with permission of Brazilian Society of Anatomy and Pan-American Association of Anatomy.
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Figure 1: Microphotograph of cross-section of human foetal sigmoid colon in NADPH-diaphorase stain at 14 weeks of gestation (WG) and 16WG. (A) Myenteric plexus (MP) at 14WG having neurons (N) with round to oval nucleus (n) and scanty cytoplasm. Neuronal processes (NP) can be clearly demarcated. Scattered neurons and thin nerve fibres present in submucosa (SM). (B) There is progressive increase in the sizes of myenteric ganglia (MG) and N at 16WG. Nerve fibres increase in inner circular muscle (CM). There is also gradually increase in neuropil. Submucosal plexus (SP) present in the SM towards the CM. LM, outer longitudinal muscle. Scale bar=50 µm (A, B). Reproduced from J Morphol Sci 2013;30:156-66 [18], with permission of Brazilian Society of Anatomy and Pan-American Association of Anatomy.

Mentions: At 14WG, the MP appeared as a continuous band of small neuronal cells. Binucleate neuronal cells were often observed. Large oval to round nuclei were there with little cytoplasm characterized the neuronal cells. Neuronal processes were observed emerging from the surface of the soma. Among the neuronal cells only simple neuropil was present at this age. Very few nerve fibers ran parallel to the circular muscle fibers. Scattered neuronal cells were present in the submucosa towards inner border of the circular muscle (Fig. 1A). At 15WG and 16WG, continuous band of cells formed into the elongated ganglia. Ganglia and neuronal cells were larger in comparison to the previous age group. There was obvious increase in the neuropil also. The nerve fibers were seen to colonize inside the circular muscle which were parallel to long axis of muscle fibers. Submucosal plexus was noted close to the circular muscle layer (Fig. 1B).


Development of myenteric plexus in human foetuses: a quantitative study.

Singh S, Shariff A, Roy T, Das T, Rani N - Anat Cell Biol (2015)

Microphotograph of cross-section of human foetal sigmoid colon in NADPH-diaphorase stain at 14 weeks of gestation (WG) and 16WG. (A) Myenteric plexus (MP) at 14WG having neurons (N) with round to oval nucleus (n) and scanty cytoplasm. Neuronal processes (NP) can be clearly demarcated. Scattered neurons and thin nerve fibres present in submucosa (SM). (B) There is progressive increase in the sizes of myenteric ganglia (MG) and N at 16WG. Nerve fibres increase in inner circular muscle (CM). There is also gradually increase in neuropil. Submucosal plexus (SP) present in the SM towards the CM. LM, outer longitudinal muscle. Scale bar=50 µm (A, B). Reproduced from J Morphol Sci 2013;30:156-66 [18], with permission of Brazilian Society of Anatomy and Pan-American Association of Anatomy.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488640&req=5

Figure 1: Microphotograph of cross-section of human foetal sigmoid colon in NADPH-diaphorase stain at 14 weeks of gestation (WG) and 16WG. (A) Myenteric plexus (MP) at 14WG having neurons (N) with round to oval nucleus (n) and scanty cytoplasm. Neuronal processes (NP) can be clearly demarcated. Scattered neurons and thin nerve fibres present in submucosa (SM). (B) There is progressive increase in the sizes of myenteric ganglia (MG) and N at 16WG. Nerve fibres increase in inner circular muscle (CM). There is also gradually increase in neuropil. Submucosal plexus (SP) present in the SM towards the CM. LM, outer longitudinal muscle. Scale bar=50 µm (A, B). Reproduced from J Morphol Sci 2013;30:156-66 [18], with permission of Brazilian Society of Anatomy and Pan-American Association of Anatomy.
Mentions: At 14WG, the MP appeared as a continuous band of small neuronal cells. Binucleate neuronal cells were often observed. Large oval to round nuclei were there with little cytoplasm characterized the neuronal cells. Neuronal processes were observed emerging from the surface of the soma. Among the neuronal cells only simple neuropil was present at this age. Very few nerve fibers ran parallel to the circular muscle fibers. Scattered neuronal cells were present in the submucosa towards inner border of the circular muscle (Fig. 1A). At 15WG and 16WG, continuous band of cells formed into the elongated ganglia. Ganglia and neuronal cells were larger in comparison to the previous age group. There was obvious increase in the neuropil also. The nerve fibers were seen to colonize inside the circular muscle which were parallel to long axis of muscle fibers. Submucosal plexus was noted close to the circular muscle layer (Fig. 1B).

Bottom Line: This study revealed that the maturational event increases after 17WG and extensive innervations is established at 23WG.During prenatal life there is an increase in the neuronal cell size from 14-23WG signifying maturational process.Such studies are essential for clinicians and surgeons to correlate the normal and pathologic development of the enteric nervous system.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, All India Institute of Medical Sciences, New Delhi, India.

ABSTRACT
Maturation of neurons of the myenteric plexus (MP) of human fetal sigmoid colon was studied at various weeks of gestation (WG). There is abundant literature on the development of MP in various segments of the gut but there are fewer reports on the development of MP in human sigmoid colon which is a site of various disorders. Sigmoid colonic segments from 12 aborted foetuses aged 14-23WG were processed for NADPH histochemistry. Stereologic evaluation of the neuronal cell profiles, numerical density, number of neurons per ganglion and myenteric fraction was conducted using using imageJ software. According to gestational age, foetuses were assigned into two groups (group 1 [n=7], less than <17WG and group 2 [n=5], more than >17WG). The overall size of neuronal cell profiles in the MP was significantly increased (P<0.05). The numerical density of neurons decreased in group 2 in comparison to group 1, the number of neurons per ganglion and myenteric fraction were increased in group 2 but all these were not statistically significant. This study revealed that the maturational event increases after 17WG and extensive innervations is established at 23WG. During prenatal life there is an increase in the neuronal cell size from 14-23WG signifying maturational process. Such studies are essential for clinicians and surgeons to correlate the normal and pathologic development of the enteric nervous system.

No MeSH data available.


Related in: MedlinePlus