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Composite nerve fibers in the hypogastric and pelvic splanchnic nerves: an immunohistochemical study using elderly cadavers.

Jang HS, Cho KH, Hieda K, Kim JH, Murakami G, Abe S, Matsubara A - Anat Cell Biol (2015)

Bottom Line: In both the HGN and PSN, the number of VIP+ fibers was usually greater than that of NOS+ fibers, with frequent co-localization of NOS and VIP.In contrast to pelvic plexus branches, there were no differences in the proportions of NOS+ and VIP+ fibers between nerve bundles in each of the tissue strips.The NOS+ and/or VIP+ fibers in the HGN were most likely ascending postganglionic fibers to the colon, while those in the PSN root may be preganglionic fibers from Onuf's nucleus.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Medical Science, Wonkwang University Graduate School, Iksan, Korea.

ABSTRACT
To determine the proportion of nerve fibers in the hypogastric nerve (HGN) and pelvic splanchnic nerve (PSN), small tissue strips of the HGN and PSN from 12 donated elderly cadavers were examined histologically. Immunohistochemistry for neuronal nitric oxide synthase (NOS), vasoactive intestinal peptide (VIP), and tyrosine hydroxylase (TH) was performed. More than 70% of fibers per bundle in the HGN were positive for TH at the level of the sacral promontory. In addition, NOS- (negative) and/or VIP+ (positive) fibers were observed in small areas of each nerve bundle, although the proportion of each was usually less than 10%. In the PSN near the third sacral nerve root, the proportion of nerve fibers positive for NOS and/or VIP (or TH) was below 30%. In both the HGN and PSN, the number of VIP+ fibers was usually greater than that of NOS+ fibers, with frequent co-localization of NOS and VIP. More fibers in both nerves were positive for TH than for these other markers. In contrast to pelvic plexus branches, there were no differences in the proportions of NOS+ and VIP+ fibers between nerve bundles in each of the tissue strips. Thus, target-dependent sorting of nerve fibers was not apparent in the HGN at the level of the sacral promontory or in the PSN near the third sacral nerve root. The NOS+ and/or VIP+ fibers in the HGN were most likely ascending postganglionic fibers to the colon, while those in the PSN root may be preganglionic fibers from Onuf's nucleus.

No MeSH data available.


Related in: MedlinePlus

Nerve bundles constituting the pelvic splanchnic nerve in a 78-year-old man. These were the same specimens as those shown in Fig. 2. Serial cross sections of two middle-sized bundles, represented by panels (A-C) and panels (D-F), respectively, were incubated with antibodies against neuronal nitric oxide synthase (NOS) (A, D), vasoactive intestinal peptide (VIP) (B, E), and tyrosine hydroxylase (TH) (C, F). In panels (A) and (B), the distributions of NOS+ and VIP+ fibers overlapped and differed from the distribution of TH+ fibers. The numbers of NOS- and VIP+ fibers were greater in panels (A) and (B) than in panels (D) and (E). The density of TH+ fibers (C, F) was much lower than in the hypogastric nerve (Fig. 2C, F). All panels were prepared at the same magnification. Scale bar=0.1 mm (A).
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Figure 5: Nerve bundles constituting the pelvic splanchnic nerve in a 78-year-old man. These were the same specimens as those shown in Fig. 2. Serial cross sections of two middle-sized bundles, represented by panels (A-C) and panels (D-F), respectively, were incubated with antibodies against neuronal nitric oxide synthase (NOS) (A, D), vasoactive intestinal peptide (VIP) (B, E), and tyrosine hydroxylase (TH) (C, F). In panels (A) and (B), the distributions of NOS+ and VIP+ fibers overlapped and differed from the distribution of TH+ fibers. The numbers of NOS- and VIP+ fibers were greater in panels (A) and (B) than in panels (D) and (E). The density of TH+ fibers (C, F) was much lower than in the hypogastric nerve (Fig. 2C, F). All panels were prepared at the same magnification. Scale bar=0.1 mm (A).

Mentions: We examined 3-4 nerve bundles in each tissue strip (Fig. 1G). Although we did not perform myelin sheath staining, such as with Luxor fast blue, the PSNs consistently contained abundant, myelinated, thick nerve fibers. Some of these thick fibers appeared positive for NOS or VIP, but all were negative for TH. There were two groups of nerve bundles: (1) NOS+ or VIP+ fiber-rich bundles of which proportion reached 20%-30% (Fig. 5) and (2) NOS+ or VIP+ fiber-poor bundles (1%-3%), with the latter type observed in only three of the 12 cadavers reason (93M, 76F and 78F in Table 2). Interestingly, the 93-year-old man with abundant NOS+ fibers in the HGN (Fig. 3) had few NOS+ fibers in the PSN (Table 2). About 20%-50% of fibers per bundle were TH+, a proportion higher than that of NOS+ or VIP+ fibers (Table 2, Fig. 5). Differences were observed in the proportions of NOS+ or VIP+ fibers among nerve bundles in each of the tissue strips (Fig. 5), but these differences did not exceed 20%. No ganglion cells were present in any of these PSN specimens. Finally, there were no gender- or age-related differences in the morphologies of the HGN and PSN.


Composite nerve fibers in the hypogastric and pelvic splanchnic nerves: an immunohistochemical study using elderly cadavers.

Jang HS, Cho KH, Hieda K, Kim JH, Murakami G, Abe S, Matsubara A - Anat Cell Biol (2015)

Nerve bundles constituting the pelvic splanchnic nerve in a 78-year-old man. These were the same specimens as those shown in Fig. 2. Serial cross sections of two middle-sized bundles, represented by panels (A-C) and panels (D-F), respectively, were incubated with antibodies against neuronal nitric oxide synthase (NOS) (A, D), vasoactive intestinal peptide (VIP) (B, E), and tyrosine hydroxylase (TH) (C, F). In panels (A) and (B), the distributions of NOS+ and VIP+ fibers overlapped and differed from the distribution of TH+ fibers. The numbers of NOS- and VIP+ fibers were greater in panels (A) and (B) than in panels (D) and (E). The density of TH+ fibers (C, F) was much lower than in the hypogastric nerve (Fig. 2C, F). All panels were prepared at the same magnification. Scale bar=0.1 mm (A).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488639&req=5

Figure 5: Nerve bundles constituting the pelvic splanchnic nerve in a 78-year-old man. These were the same specimens as those shown in Fig. 2. Serial cross sections of two middle-sized bundles, represented by panels (A-C) and panels (D-F), respectively, were incubated with antibodies against neuronal nitric oxide synthase (NOS) (A, D), vasoactive intestinal peptide (VIP) (B, E), and tyrosine hydroxylase (TH) (C, F). In panels (A) and (B), the distributions of NOS+ and VIP+ fibers overlapped and differed from the distribution of TH+ fibers. The numbers of NOS- and VIP+ fibers were greater in panels (A) and (B) than in panels (D) and (E). The density of TH+ fibers (C, F) was much lower than in the hypogastric nerve (Fig. 2C, F). All panels were prepared at the same magnification. Scale bar=0.1 mm (A).
Mentions: We examined 3-4 nerve bundles in each tissue strip (Fig. 1G). Although we did not perform myelin sheath staining, such as with Luxor fast blue, the PSNs consistently contained abundant, myelinated, thick nerve fibers. Some of these thick fibers appeared positive for NOS or VIP, but all were negative for TH. There were two groups of nerve bundles: (1) NOS+ or VIP+ fiber-rich bundles of which proportion reached 20%-30% (Fig. 5) and (2) NOS+ or VIP+ fiber-poor bundles (1%-3%), with the latter type observed in only three of the 12 cadavers reason (93M, 76F and 78F in Table 2). Interestingly, the 93-year-old man with abundant NOS+ fibers in the HGN (Fig. 3) had few NOS+ fibers in the PSN (Table 2). About 20%-50% of fibers per bundle were TH+, a proportion higher than that of NOS+ or VIP+ fibers (Table 2, Fig. 5). Differences were observed in the proportions of NOS+ or VIP+ fibers among nerve bundles in each of the tissue strips (Fig. 5), but these differences did not exceed 20%. No ganglion cells were present in any of these PSN specimens. Finally, there were no gender- or age-related differences in the morphologies of the HGN and PSN.

Bottom Line: In both the HGN and PSN, the number of VIP+ fibers was usually greater than that of NOS+ fibers, with frequent co-localization of NOS and VIP.In contrast to pelvic plexus branches, there were no differences in the proportions of NOS+ and VIP+ fibers between nerve bundles in each of the tissue strips.The NOS+ and/or VIP+ fibers in the HGN were most likely ascending postganglionic fibers to the colon, while those in the PSN root may be preganglionic fibers from Onuf's nucleus.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Medical Science, Wonkwang University Graduate School, Iksan, Korea.

ABSTRACT
To determine the proportion of nerve fibers in the hypogastric nerve (HGN) and pelvic splanchnic nerve (PSN), small tissue strips of the HGN and PSN from 12 donated elderly cadavers were examined histologically. Immunohistochemistry for neuronal nitric oxide synthase (NOS), vasoactive intestinal peptide (VIP), and tyrosine hydroxylase (TH) was performed. More than 70% of fibers per bundle in the HGN were positive for TH at the level of the sacral promontory. In addition, NOS- (negative) and/or VIP+ (positive) fibers were observed in small areas of each nerve bundle, although the proportion of each was usually less than 10%. In the PSN near the third sacral nerve root, the proportion of nerve fibers positive for NOS and/or VIP (or TH) was below 30%. In both the HGN and PSN, the number of VIP+ fibers was usually greater than that of NOS+ fibers, with frequent co-localization of NOS and VIP. More fibers in both nerves were positive for TH than for these other markers. In contrast to pelvic plexus branches, there were no differences in the proportions of NOS+ and VIP+ fibers between nerve bundles in each of the tissue strips. Thus, target-dependent sorting of nerve fibers was not apparent in the HGN at the level of the sacral promontory or in the PSN near the third sacral nerve root. The NOS+ and/or VIP+ fibers in the HGN were most likely ascending postganglionic fibers to the colon, while those in the PSN root may be preganglionic fibers from Onuf's nucleus.

No MeSH data available.


Related in: MedlinePlus