Limits...
Proteome Analysis for Understanding Abiotic Stress (Salinity and Drought) Tolerance in Date Palm (Phoenix dactylifera L.).

El Rabey HA, Al-Malki AL, Abulnaja KO, Rohde W - Int J Genomics (2015)

Bottom Line: Mass spectrometric analysis identified 12 proteins; three out of them were significantly changed under both salt and drought stress, while the other nine were significantly changed only in salt-stressed plants.The levels of ATP synthase alpha and beta subunits, an unknown protein and some of RubisCO fragments were significantly changed under both salt and drought stress conditions.Changes in abundance of superoxide dismutase, chlorophyll A-B binding protein, light-harvesting complex1 protein Lhca1, RubisCO activase, phosphoglycerate kinase, chloroplast light-harvesting chlorophyll a/b-binding protein, phosphoribulokinase, transketolase, RubisCO, and some of RubisCO fragments were significant only for salt stress.

View Article: PubMed Central - PubMed

Affiliation: Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia ; Bioinformatics Department, Genetic Engineering and Biotechnology Institute, Sadat City University, Sadat City, Minufiya, Egypt.

ABSTRACT
This study was carried out to study the proteome of date palm under salinity and drought stress conditions to possibly identify proteins involved in stress tolerance. For this purpose, three-month-old seedlings of date palm cultivar "Sagie" were subjected to drought (27.5 g/L polyethylene glycol 6000) and salinity stress conditions (16 g/L NaCl) for one month. DIGE analysis of protein extracts identified 47 differentially expressed proteins in leaves of salt- and drought-treated palm seedlings. Mass spectrometric analysis identified 12 proteins; three out of them were significantly changed under both salt and drought stress, while the other nine were significantly changed only in salt-stressed plants. The levels of ATP synthase alpha and beta subunits, an unknown protein and some of RubisCO fragments were significantly changed under both salt and drought stress conditions. Changes in abundance of superoxide dismutase, chlorophyll A-B binding protein, light-harvesting complex1 protein Lhca1, RubisCO activase, phosphoglycerate kinase, chloroplast light-harvesting chlorophyll a/b-binding protein, phosphoribulokinase, transketolase, RubisCO, and some of RubisCO fragments were significant only for salt stress.

No MeSH data available.


Related in: MedlinePlus

Fluorescence scans of control (C) versus salt stress (SS): spots shown in red had higher standardized abundance in control compared to salt stress and blue color highlighted the spots with lower abundance. Red marked regions contained intensive spots significantly decreased in abundance in salt stress sample, and the blue one shows increased abundance.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4488584&req=5

fig3: Fluorescence scans of control (C) versus salt stress (SS): spots shown in red had higher standardized abundance in control compared to salt stress and blue color highlighted the spots with lower abundance. Red marked regions contained intensive spots significantly decreased in abundance in salt stress sample, and the blue one shows increased abundance.

Mentions: For this analysis, the standardized abundance of each spot on the gel was calculated for two samples in the gel using the spot intensities of the internal standard. Significant differences between the samples were visualized showing blue or red spot contours for higher or lower standardized abundance (threshold 3) of the spots, respectively. Regions with intensive spots changing significantly were manually marked. Figure 3 shows fluorescence scan of the control versus salt stress, Figure 4 shows fluorescence scan of control versus drought stress, and Figure 5 shows fluorescence scan of the salt stress versus drought stress.


Proteome Analysis for Understanding Abiotic Stress (Salinity and Drought) Tolerance in Date Palm (Phoenix dactylifera L.).

El Rabey HA, Al-Malki AL, Abulnaja KO, Rohde W - Int J Genomics (2015)

Fluorescence scans of control (C) versus salt stress (SS): spots shown in red had higher standardized abundance in control compared to salt stress and blue color highlighted the spots with lower abundance. Red marked regions contained intensive spots significantly decreased in abundance in salt stress sample, and the blue one shows increased abundance.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4488584&req=5

fig3: Fluorescence scans of control (C) versus salt stress (SS): spots shown in red had higher standardized abundance in control compared to salt stress and blue color highlighted the spots with lower abundance. Red marked regions contained intensive spots significantly decreased in abundance in salt stress sample, and the blue one shows increased abundance.
Mentions: For this analysis, the standardized abundance of each spot on the gel was calculated for two samples in the gel using the spot intensities of the internal standard. Significant differences between the samples were visualized showing blue or red spot contours for higher or lower standardized abundance (threshold 3) of the spots, respectively. Regions with intensive spots changing significantly were manually marked. Figure 3 shows fluorescence scan of the control versus salt stress, Figure 4 shows fluorescence scan of control versus drought stress, and Figure 5 shows fluorescence scan of the salt stress versus drought stress.

Bottom Line: Mass spectrometric analysis identified 12 proteins; three out of them were significantly changed under both salt and drought stress, while the other nine were significantly changed only in salt-stressed plants.The levels of ATP synthase alpha and beta subunits, an unknown protein and some of RubisCO fragments were significantly changed under both salt and drought stress conditions.Changes in abundance of superoxide dismutase, chlorophyll A-B binding protein, light-harvesting complex1 protein Lhca1, RubisCO activase, phosphoglycerate kinase, chloroplast light-harvesting chlorophyll a/b-binding protein, phosphoribulokinase, transketolase, RubisCO, and some of RubisCO fragments were significant only for salt stress.

View Article: PubMed Central - PubMed

Affiliation: Biochemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia ; Bioinformatics Department, Genetic Engineering and Biotechnology Institute, Sadat City University, Sadat City, Minufiya, Egypt.

ABSTRACT
This study was carried out to study the proteome of date palm under salinity and drought stress conditions to possibly identify proteins involved in stress tolerance. For this purpose, three-month-old seedlings of date palm cultivar "Sagie" were subjected to drought (27.5 g/L polyethylene glycol 6000) and salinity stress conditions (16 g/L NaCl) for one month. DIGE analysis of protein extracts identified 47 differentially expressed proteins in leaves of salt- and drought-treated palm seedlings. Mass spectrometric analysis identified 12 proteins; three out of them were significantly changed under both salt and drought stress, while the other nine were significantly changed only in salt-stressed plants. The levels of ATP synthase alpha and beta subunits, an unknown protein and some of RubisCO fragments were significantly changed under both salt and drought stress conditions. Changes in abundance of superoxide dismutase, chlorophyll A-B binding protein, light-harvesting complex1 protein Lhca1, RubisCO activase, phosphoglycerate kinase, chloroplast light-harvesting chlorophyll a/b-binding protein, phosphoribulokinase, transketolase, RubisCO, and some of RubisCO fragments were significant only for salt stress.

No MeSH data available.


Related in: MedlinePlus