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Heat-Processed Scutellariae Radix Enhances Anti-Inflammatory Effect against Lipopolysaccharide-Induced Acute Lung Injury in Mice via NF- κ B Signaling.

Shin YO, Park CH, Lee GH, Yokozawa T, Roh SS, Rhee MH - Evid Based Complement Alternat Med (2015)

Bottom Line: The effects of Scutellariae Radix heat-processed at 160°C (HSR) were compared with those of nonheat-processed Scutellariae Radix (NSR).The reduced superoxide dismutase and catalase increased significantly by both NSR and HSR.Overall, the ameliorative effects of HSR were superior to those when being nonheat-processed.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.

ABSTRACT
The present study was conducted to examine whether heat-processed Scutellariae Radix has an ameliorative effect on lipopolysaccharide- (LPS-) induced acute lung injury in mice. The effects of Scutellariae Radix heat-processed at 160°C (HSR) were compared with those of nonheat-processed Scutellariae Radix (NSR). The LPS-treated group displayed a markedly decreased body weight and significantly increased lung weight; however, the administration of NSR or HSR improved both the body and lung weights. The increased oxidative stress and inflammatory biomarker levels in the serum and lung were reduced significantly with HSR. The reduced superoxide dismutase and catalase increased significantly by both NSR and HSR. Also, the dysregulated oxidative stress and inflammation were significantly ameliorated by NSR and HSR. The expression of inflammatory mediators and cytokines by nuclear factor-kappa B activation was modulated through inhibition of a nuclear factor kappa Bα degradation. Also, lung histological change was markedly suppressed by HSR rather than NSR. Overall, the ameliorative effects of HSR were superior to those when being nonheat-processed. The representative flavonoid contents of Scutellariae Radix that include baicalin, baicalein, and wogonin were greater by heat process. These data reveal heat-processed Scutellariae Radix may be a critical factor involved in the improvement of lung disorders caused by LPS.

No MeSH data available.


Related in: MedlinePlus

IκBα (a), IκBβ (b), and NF-κBp65 (c) protein expressions in lung tissue. N, normal mice; Veh., vehicle-administered and LPS-treated mice; NSR, nonheat-processed Scutellariae Radix-administered and LPS-treated mice; HSR, heat-processed Scutellariae Radix-administered and LPS-treated mice. Data are the mean ± SEM. Significance: ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 versus vehicle-administered and LPS-treated mice.
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fig4: IκBα (a), IκBβ (b), and NF-κBp65 (c) protein expressions in lung tissue. N, normal mice; Veh., vehicle-administered and LPS-treated mice; NSR, nonheat-processed Scutellariae Radix-administered and LPS-treated mice; HSR, heat-processed Scutellariae Radix-administered and LPS-treated mice. Data are the mean ± SEM. Significance: ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 versus vehicle-administered and LPS-treated mice.

Mentions: The protein levels of the oxidative stress-related proteins, such as IκBα, IκBβ, and NF-κBp65, were examined. In the vehicle-administered and LPS-treated mice, the protein expression of IκBα was significantly reduced and that of IκBβ was slightly reduced compared with the normal group (Figures 4(a) and 4(b)). Nonheat-processed Scutellariae Radix administration showed no difference compared with vehicle-administered acute lung injury mice, and heat-processed Scutellariae Radix led to a significant upregulation of IκBα protein expression. Moreover, the vehicle-administered and LPS-treated mice showed upregulation of the nuclear NF-κBp65 protein compared to normal mice. On the other hand, the administration of nonheat-processed and heat-processed Scutellariae Radix led to a significant downregulation of NF-κBp65 protein expression (Figure 4(c)).


Heat-Processed Scutellariae Radix Enhances Anti-Inflammatory Effect against Lipopolysaccharide-Induced Acute Lung Injury in Mice via NF- κ B Signaling.

Shin YO, Park CH, Lee GH, Yokozawa T, Roh SS, Rhee MH - Evid Based Complement Alternat Med (2015)

IκBα (a), IκBβ (b), and NF-κBp65 (c) protein expressions in lung tissue. N, normal mice; Veh., vehicle-administered and LPS-treated mice; NSR, nonheat-processed Scutellariae Radix-administered and LPS-treated mice; HSR, heat-processed Scutellariae Radix-administered and LPS-treated mice. Data are the mean ± SEM. Significance: ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 versus vehicle-administered and LPS-treated mice.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4488546&req=5

fig4: IκBα (a), IκBβ (b), and NF-κBp65 (c) protein expressions in lung tissue. N, normal mice; Veh., vehicle-administered and LPS-treated mice; NSR, nonheat-processed Scutellariae Radix-administered and LPS-treated mice; HSR, heat-processed Scutellariae Radix-administered and LPS-treated mice. Data are the mean ± SEM. Significance: ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 versus vehicle-administered and LPS-treated mice.
Mentions: The protein levels of the oxidative stress-related proteins, such as IκBα, IκBβ, and NF-κBp65, were examined. In the vehicle-administered and LPS-treated mice, the protein expression of IκBα was significantly reduced and that of IκBβ was slightly reduced compared with the normal group (Figures 4(a) and 4(b)). Nonheat-processed Scutellariae Radix administration showed no difference compared with vehicle-administered acute lung injury mice, and heat-processed Scutellariae Radix led to a significant upregulation of IκBα protein expression. Moreover, the vehicle-administered and LPS-treated mice showed upregulation of the nuclear NF-κBp65 protein compared to normal mice. On the other hand, the administration of nonheat-processed and heat-processed Scutellariae Radix led to a significant downregulation of NF-κBp65 protein expression (Figure 4(c)).

Bottom Line: The effects of Scutellariae Radix heat-processed at 160°C (HSR) were compared with those of nonheat-processed Scutellariae Radix (NSR).The reduced superoxide dismutase and catalase increased significantly by both NSR and HSR.Overall, the ameliorative effects of HSR were superior to those when being nonheat-processed.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.

ABSTRACT
The present study was conducted to examine whether heat-processed Scutellariae Radix has an ameliorative effect on lipopolysaccharide- (LPS-) induced acute lung injury in mice. The effects of Scutellariae Radix heat-processed at 160°C (HSR) were compared with those of nonheat-processed Scutellariae Radix (NSR). The LPS-treated group displayed a markedly decreased body weight and significantly increased lung weight; however, the administration of NSR or HSR improved both the body and lung weights. The increased oxidative stress and inflammatory biomarker levels in the serum and lung were reduced significantly with HSR. The reduced superoxide dismutase and catalase increased significantly by both NSR and HSR. Also, the dysregulated oxidative stress and inflammation were significantly ameliorated by NSR and HSR. The expression of inflammatory mediators and cytokines by nuclear factor-kappa B activation was modulated through inhibition of a nuclear factor kappa Bα degradation. Also, lung histological change was markedly suppressed by HSR rather than NSR. Overall, the ameliorative effects of HSR were superior to those when being nonheat-processed. The representative flavonoid contents of Scutellariae Radix that include baicalin, baicalein, and wogonin were greater by heat process. These data reveal heat-processed Scutellariae Radix may be a critical factor involved in the improvement of lung disorders caused by LPS.

No MeSH data available.


Related in: MedlinePlus