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Neuroprotective Properties of the Standardized Extract from Camellia sinensis (Green Tea) and Its Main Bioactive Components, Epicatechin and Epigallocatechin Gallate, in the 6-OHDA Model of Parkinson's Disease.

Bitu Pinto N, da Silva Alexandre B, Neves KR, Silva AH, Leal LK, Viana GS - Evid Based Complement Alternat Med (2015)

Bottom Line: The results showed that CS and catechins reverted behavioral changes, indicating neuroprotection manifested as decreased rotational behavior, increased locomotor activity, antidepressive effects, and improvement of cognitive dysfunction, as compared to the untreated 6-OHDA-lesioned group.Besides, CS, EP, and EGCG reversed the striatal oxidative stress and immunohistochemistry alterations.These results show that the neuroprotective effects of CS and its catechins are probably and in great part due to its powerful antioxidant and anti-inflammatory properties, pointing out their potential for the prevention and treatment of PD.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Medicine of the Federal University of Ceará, Rua Nunes de Melo 1127 (Rodolfo Teófilo), 60430-270 Fortaleza, CE, Brazil ; Faculty of Medicine Estácio of Juazeiro do Norte, Avenida Tenente Raimundo Rocha 515 (Cidade Universitária), 63048-080 Juazeiro do Norte, CE, Brazil.

ABSTRACT
Camellia sinensis (green tea) is largely consumed, mainly in Asia. It possesses several biological effects such as antioxidant and anti-inflammatory properties. The objectives were to investigate the neuroprotective actions of the standardized extract (CS), epicatechin (EC) and epigallocatechin gallate (EGCG), on a model of Parkinson's disease. Male Wistar rats were divided into SO (sham-operated controls), untreated 6-OHDA-lesioned and 6-OHDA-lesioned treated for 2 weeks with CS (25, 50, or 100 mg/kg), EC (10 mg/kg), or EGCG (10 mg/kg) groups. One hour after the last administration, animals were submitted to behavioral tests and euthanized and their striata and hippocampi were dissected for neurochemical (DA, DOPAC, and HVA) and antioxidant activity determinations, as well as immunohistochemistry evaluations (TH, COX-2, and iNOS). The results showed that CS and catechins reverted behavioral changes, indicating neuroprotection manifested as decreased rotational behavior, increased locomotor activity, antidepressive effects, and improvement of cognitive dysfunction, as compared to the untreated 6-OHDA-lesioned group. Besides, CS, EP, and EGCG reversed the striatal oxidative stress and immunohistochemistry alterations. These results show that the neuroprotective effects of CS and its catechins are probably and in great part due to its powerful antioxidant and anti-inflammatory properties, pointing out their potential for the prevention and treatment of PD.

No MeSH data available.


Related in: MedlinePlus

Treatments with CS25 or EGCG10 of 6-OHDA-lesioned animals partly reversed the drastic increase in immunostaining for COX-2 in the striata. The data were quantified by the Image J software. a: versus SO, q = 12.27; b: versus 6-OHDA + CS25, q = 6.07; c: versus 6-OHDA + EGCG10, q = 6.68; d: versus SO, q = 7.12; e: versus SO, q = 6.47 (one-way ANOVA and Newman-Keuls as the post hoc test).
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fig10: Treatments with CS25 or EGCG10 of 6-OHDA-lesioned animals partly reversed the drastic increase in immunostaining for COX-2 in the striata. The data were quantified by the Image J software. a: versus SO, q = 12.27; b: versus 6-OHDA + CS25, q = 6.07; c: versus 6-OHDA + EGCG10, q = 6.68; d: versus SO, q = 7.12; e: versus SO, q = 6.47 (one-way ANOVA and Newman-Keuls as the post hoc test).

Mentions: The untreated 6-OHDA group showed around 80% decreases in striatal immunostaining for TH (Figure 9), as related to the SO group. However, the results observed in the 6-OHDA group after treatment with CS (6-OHDA + CS25) presented immunostaining similar to that observed in the SO group. A 4-fold increase in COX-2 immunostaining (Figure 10) was observed in striatal tissue from untreated 6-OHDA animals, as related to the SO group. In addition, increases of only 2.7- and 2.5-fold were observed in the 6-OHDA groups after treatments with CS25 or EGCG10. Immunohistochemistry assays were also performed for hippocampal tissues in these same groups. The results showed around 24-, 129-, and 10-fold increases in immunostainings for iNOS, in CA1, CA3, and DG subfields in the untreated 6-OHDA animals, as related to the SO group. The values decreased towards those of the SO group in the 6-OHDA group after treatments with CS25 and EGCG10, mainly at CA1 and CA3 areas (Figure 11).


Neuroprotective Properties of the Standardized Extract from Camellia sinensis (Green Tea) and Its Main Bioactive Components, Epicatechin and Epigallocatechin Gallate, in the 6-OHDA Model of Parkinson's Disease.

Bitu Pinto N, da Silva Alexandre B, Neves KR, Silva AH, Leal LK, Viana GS - Evid Based Complement Alternat Med (2015)

Treatments with CS25 or EGCG10 of 6-OHDA-lesioned animals partly reversed the drastic increase in immunostaining for COX-2 in the striata. The data were quantified by the Image J software. a: versus SO, q = 12.27; b: versus 6-OHDA + CS25, q = 6.07; c: versus 6-OHDA + EGCG10, q = 6.68; d: versus SO, q = 7.12; e: versus SO, q = 6.47 (one-way ANOVA and Newman-Keuls as the post hoc test).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4488543&req=5

fig10: Treatments with CS25 or EGCG10 of 6-OHDA-lesioned animals partly reversed the drastic increase in immunostaining for COX-2 in the striata. The data were quantified by the Image J software. a: versus SO, q = 12.27; b: versus 6-OHDA + CS25, q = 6.07; c: versus 6-OHDA + EGCG10, q = 6.68; d: versus SO, q = 7.12; e: versus SO, q = 6.47 (one-way ANOVA and Newman-Keuls as the post hoc test).
Mentions: The untreated 6-OHDA group showed around 80% decreases in striatal immunostaining for TH (Figure 9), as related to the SO group. However, the results observed in the 6-OHDA group after treatment with CS (6-OHDA + CS25) presented immunostaining similar to that observed in the SO group. A 4-fold increase in COX-2 immunostaining (Figure 10) was observed in striatal tissue from untreated 6-OHDA animals, as related to the SO group. In addition, increases of only 2.7- and 2.5-fold were observed in the 6-OHDA groups after treatments with CS25 or EGCG10. Immunohistochemistry assays were also performed for hippocampal tissues in these same groups. The results showed around 24-, 129-, and 10-fold increases in immunostainings for iNOS, in CA1, CA3, and DG subfields in the untreated 6-OHDA animals, as related to the SO group. The values decreased towards those of the SO group in the 6-OHDA group after treatments with CS25 and EGCG10, mainly at CA1 and CA3 areas (Figure 11).

Bottom Line: The results showed that CS and catechins reverted behavioral changes, indicating neuroprotection manifested as decreased rotational behavior, increased locomotor activity, antidepressive effects, and improvement of cognitive dysfunction, as compared to the untreated 6-OHDA-lesioned group.Besides, CS, EP, and EGCG reversed the striatal oxidative stress and immunohistochemistry alterations.These results show that the neuroprotective effects of CS and its catechins are probably and in great part due to its powerful antioxidant and anti-inflammatory properties, pointing out their potential for the prevention and treatment of PD.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Medicine of the Federal University of Ceará, Rua Nunes de Melo 1127 (Rodolfo Teófilo), 60430-270 Fortaleza, CE, Brazil ; Faculty of Medicine Estácio of Juazeiro do Norte, Avenida Tenente Raimundo Rocha 515 (Cidade Universitária), 63048-080 Juazeiro do Norte, CE, Brazil.

ABSTRACT
Camellia sinensis (green tea) is largely consumed, mainly in Asia. It possesses several biological effects such as antioxidant and anti-inflammatory properties. The objectives were to investigate the neuroprotective actions of the standardized extract (CS), epicatechin (EC) and epigallocatechin gallate (EGCG), on a model of Parkinson's disease. Male Wistar rats were divided into SO (sham-operated controls), untreated 6-OHDA-lesioned and 6-OHDA-lesioned treated for 2 weeks with CS (25, 50, or 100 mg/kg), EC (10 mg/kg), or EGCG (10 mg/kg) groups. One hour after the last administration, animals were submitted to behavioral tests and euthanized and their striata and hippocampi were dissected for neurochemical (DA, DOPAC, and HVA) and antioxidant activity determinations, as well as immunohistochemistry evaluations (TH, COX-2, and iNOS). The results showed that CS and catechins reverted behavioral changes, indicating neuroprotection manifested as decreased rotational behavior, increased locomotor activity, antidepressive effects, and improvement of cognitive dysfunction, as compared to the untreated 6-OHDA-lesioned group. Besides, CS, EP, and EGCG reversed the striatal oxidative stress and immunohistochemistry alterations. These results show that the neuroprotective effects of CS and its catechins are probably and in great part due to its powerful antioxidant and anti-inflammatory properties, pointing out their potential for the prevention and treatment of PD.

No MeSH data available.


Related in: MedlinePlus