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Neuroprotective Properties of the Standardized Extract from Camellia sinensis (Green Tea) and Its Main Bioactive Components, Epicatechin and Epigallocatechin Gallate, in the 6-OHDA Model of Parkinson's Disease.

Bitu Pinto N, da Silva Alexandre B, Neves KR, Silva AH, Leal LK, Viana GS - Evid Based Complement Alternat Med (2015)

Bottom Line: The results showed that CS and catechins reverted behavioral changes, indicating neuroprotection manifested as decreased rotational behavior, increased locomotor activity, antidepressive effects, and improvement of cognitive dysfunction, as compared to the untreated 6-OHDA-lesioned group.Besides, CS, EP, and EGCG reversed the striatal oxidative stress and immunohistochemistry alterations.These results show that the neuroprotective effects of CS and its catechins are probably and in great part due to its powerful antioxidant and anti-inflammatory properties, pointing out their potential for the prevention and treatment of PD.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Medicine of the Federal University of Ceará, Rua Nunes de Melo 1127 (Rodolfo Teófilo), 60430-270 Fortaleza, CE, Brazil ; Faculty of Medicine Estácio of Juazeiro do Norte, Avenida Tenente Raimundo Rocha 515 (Cidade Universitária), 63048-080 Juazeiro do Norte, CE, Brazil.

ABSTRACT
Camellia sinensis (green tea) is largely consumed, mainly in Asia. It possesses several biological effects such as antioxidant and anti-inflammatory properties. The objectives were to investigate the neuroprotective actions of the standardized extract (CS), epicatechin (EC) and epigallocatechin gallate (EGCG), on a model of Parkinson's disease. Male Wistar rats were divided into SO (sham-operated controls), untreated 6-OHDA-lesioned and 6-OHDA-lesioned treated for 2 weeks with CS (25, 50, or 100 mg/kg), EC (10 mg/kg), or EGCG (10 mg/kg) groups. One hour after the last administration, animals were submitted to behavioral tests and euthanized and their striata and hippocampi were dissected for neurochemical (DA, DOPAC, and HVA) and antioxidant activity determinations, as well as immunohistochemistry evaluations (TH, COX-2, and iNOS). The results showed that CS and catechins reverted behavioral changes, indicating neuroprotection manifested as decreased rotational behavior, increased locomotor activity, antidepressive effects, and improvement of cognitive dysfunction, as compared to the untreated 6-OHDA-lesioned group. Besides, CS, EP, and EGCG reversed the striatal oxidative stress and immunohistochemistry alterations. These results show that the neuroprotective effects of CS and its catechins are probably and in great part due to its powerful antioxidant and anti-inflammatory properties, pointing out their potential for the prevention and treatment of PD.

No MeSH data available.


Related in: MedlinePlus

Effects presented by the 6-OHDA-lesioned animals, before and after treatments with CS25, CS50, EC10, or EGCG10, on the time (s) to find the platform as assessed by the water maze test. a: versus SO, q = 5.92; b: versus 6-OHDA + CS50, q = 4.54; c: versus 6-OHDA + EC10, q = 3.71 (one-way ANOVA and Newman-Keuls as the post hoc test).
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fig4: Effects presented by the 6-OHDA-lesioned animals, before and after treatments with CS25, CS50, EC10, or EGCG10, on the time (s) to find the platform as assessed by the water maze test. a: versus SO, q = 5.92; b: versus 6-OHDA + CS50, q = 4.54; c: versus 6-OHDA + EC10, q = 3.71 (one-way ANOVA and Newman-Keuls as the post hoc test).

Mentions: We demonstrated (Figure 4) a 3.2-fold increase in the time to find the platform by the untreated 6-OHDA group, as related to the SO, indicating an alteration of the spacial memory and hippocampal dysfunction. The increase was lower in the 6-OHDA groups after treatments with CS25 and CS50 (2.4- and 1.6-fold, resp.). In the 6-OHDA groups treated with EC10 and EGCG10 the values were close to those of the SO group.


Neuroprotective Properties of the Standardized Extract from Camellia sinensis (Green Tea) and Its Main Bioactive Components, Epicatechin and Epigallocatechin Gallate, in the 6-OHDA Model of Parkinson's Disease.

Bitu Pinto N, da Silva Alexandre B, Neves KR, Silva AH, Leal LK, Viana GS - Evid Based Complement Alternat Med (2015)

Effects presented by the 6-OHDA-lesioned animals, before and after treatments with CS25, CS50, EC10, or EGCG10, on the time (s) to find the platform as assessed by the water maze test. a: versus SO, q = 5.92; b: versus 6-OHDA + CS50, q = 4.54; c: versus 6-OHDA + EC10, q = 3.71 (one-way ANOVA and Newman-Keuls as the post hoc test).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4488543&req=5

fig4: Effects presented by the 6-OHDA-lesioned animals, before and after treatments with CS25, CS50, EC10, or EGCG10, on the time (s) to find the platform as assessed by the water maze test. a: versus SO, q = 5.92; b: versus 6-OHDA + CS50, q = 4.54; c: versus 6-OHDA + EC10, q = 3.71 (one-way ANOVA and Newman-Keuls as the post hoc test).
Mentions: We demonstrated (Figure 4) a 3.2-fold increase in the time to find the platform by the untreated 6-OHDA group, as related to the SO, indicating an alteration of the spacial memory and hippocampal dysfunction. The increase was lower in the 6-OHDA groups after treatments with CS25 and CS50 (2.4- and 1.6-fold, resp.). In the 6-OHDA groups treated with EC10 and EGCG10 the values were close to those of the SO group.

Bottom Line: The results showed that CS and catechins reverted behavioral changes, indicating neuroprotection manifested as decreased rotational behavior, increased locomotor activity, antidepressive effects, and improvement of cognitive dysfunction, as compared to the untreated 6-OHDA-lesioned group.Besides, CS, EP, and EGCG reversed the striatal oxidative stress and immunohistochemistry alterations.These results show that the neuroprotective effects of CS and its catechins are probably and in great part due to its powerful antioxidant and anti-inflammatory properties, pointing out their potential for the prevention and treatment of PD.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Medicine of the Federal University of Ceará, Rua Nunes de Melo 1127 (Rodolfo Teófilo), 60430-270 Fortaleza, CE, Brazil ; Faculty of Medicine Estácio of Juazeiro do Norte, Avenida Tenente Raimundo Rocha 515 (Cidade Universitária), 63048-080 Juazeiro do Norte, CE, Brazil.

ABSTRACT
Camellia sinensis (green tea) is largely consumed, mainly in Asia. It possesses several biological effects such as antioxidant and anti-inflammatory properties. The objectives were to investigate the neuroprotective actions of the standardized extract (CS), epicatechin (EC) and epigallocatechin gallate (EGCG), on a model of Parkinson's disease. Male Wistar rats were divided into SO (sham-operated controls), untreated 6-OHDA-lesioned and 6-OHDA-lesioned treated for 2 weeks with CS (25, 50, or 100 mg/kg), EC (10 mg/kg), or EGCG (10 mg/kg) groups. One hour after the last administration, animals were submitted to behavioral tests and euthanized and their striata and hippocampi were dissected for neurochemical (DA, DOPAC, and HVA) and antioxidant activity determinations, as well as immunohistochemistry evaluations (TH, COX-2, and iNOS). The results showed that CS and catechins reverted behavioral changes, indicating neuroprotection manifested as decreased rotational behavior, increased locomotor activity, antidepressive effects, and improvement of cognitive dysfunction, as compared to the untreated 6-OHDA-lesioned group. Besides, CS, EP, and EGCG reversed the striatal oxidative stress and immunohistochemistry alterations. These results show that the neuroprotective effects of CS and its catechins are probably and in great part due to its powerful antioxidant and anti-inflammatory properties, pointing out their potential for the prevention and treatment of PD.

No MeSH data available.


Related in: MedlinePlus