Limits...
Diet affects the redox system in developing Atlantic cod (Gadus morhua) larvae.

Penglase S, Edvardsen RB, Furmanek T, Rønnestad I, Karlsen Ø, van der Meeren T, Hamre K - Redox Biol (2015)

Bottom Line: The growth and development of marine fish larvae fed copepods is superior to those fed rotifers, but the underlying molecular reasons for this are unclear.The oxidised and reduced glutathione levels, the redox potential, and the mRNA expression of 100 genes in redox system pathways were then compared between treatments during larval development.We found that rotifer/Artemia-fed cod larvae had lower levels of oxidised glutathione, a more reduced redox potential, and altered expression of approximately half of the redox system genes when compared to copepod-fed larvae.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Nutrition and Seafood Research (NIFES), PO Box 2029, NO-5817 Bergen, Norway.

No MeSH data available.


The effect of diet on the expression of genes in thiol oxidoreductase pathways in cod larvae.The mRNA expression of glutaredoxin (A), thioredoxin-like 1 (B), 4 (C), thioredoxin interacting protein a (D), b (E), thioredoxin reductase 1 (F), 3 (G) and sulfiredoxin 1 (H) in developing cod larvae fed either rotifers (□ red line) or copepods (○ blue line). Shaded areas cover life stages that copepod-fed larvae had elevated growth rates compared to rotifer-fed larvae. Letters indicate statistical relationships between all data points, with ⁎ and p values indicating statistical factorial and main effects, respectively, of diet (p<0.05). See Fig. 1 for more details. Data are mean±SEM, n=3.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4488531&req=5

f0030: The effect of diet on the expression of genes in thiol oxidoreductase pathways in cod larvae.The mRNA expression of glutaredoxin (A), thioredoxin-like 1 (B), 4 (C), thioredoxin interacting protein a (D), b (E), thioredoxin reductase 1 (F), 3 (G) and sulfiredoxin 1 (H) in developing cod larvae fed either rotifers (□ red line) or copepods (○ blue line). Shaded areas cover life stages that copepod-fed larvae had elevated growth rates compared to rotifer-fed larvae. Letters indicate statistical relationships between all data points, with ⁎ and p values indicating statistical factorial and main effects, respectively, of diet (p<0.05). See Fig. 1 for more details. Data are mean±SEM, n=3.

Mentions: Over half (57%, Table 1) of the analysed genes coding for proteins that reduce oxidised cysteine residues (thiol oxidoreductases), or interact directly with these enzymes (thioredoxin interacting proteins; txnip’s) were affected by the diets (Fig. 6). Of these, the txnrd’s (1 and 3), txnipa and srxn1 were upregulated, while glrx, txnl1, txnl4 and txnipb were downregulated at either a single development stage or as a main effect in rotifer-fed larvae (Fig. 6). The largest number of differences induced by the diets was observed at development stage 3 (3 out of 5 factorial effects, Fig. 6). Only txnl1 in copepod-fed larvae (Fig. 6B) was dynamically expressed (up then down regulated) during development. With this exception, any changes in gene regulation that occurred in either treatment with development generally persisted until the last stage analysed. For example txnrd3 was upregulated at stage 2 in rotifer fed larvae (p<0.05), from where it remained upregulated until stage 5 (Fig. 6G). The expression of several genes; txnrd1, srxn1 did not change due to development (p>0.05) in either diet group (Fig. 6F and H), or changed late in development, for example txnipa and b in copepod-fed fish, and glrx for both diets (A, D and E).


Diet affects the redox system in developing Atlantic cod (Gadus morhua) larvae.

Penglase S, Edvardsen RB, Furmanek T, Rønnestad I, Karlsen Ø, van der Meeren T, Hamre K - Redox Biol (2015)

The effect of diet on the expression of genes in thiol oxidoreductase pathways in cod larvae.The mRNA expression of glutaredoxin (A), thioredoxin-like 1 (B), 4 (C), thioredoxin interacting protein a (D), b (E), thioredoxin reductase 1 (F), 3 (G) and sulfiredoxin 1 (H) in developing cod larvae fed either rotifers (□ red line) or copepods (○ blue line). Shaded areas cover life stages that copepod-fed larvae had elevated growth rates compared to rotifer-fed larvae. Letters indicate statistical relationships between all data points, with ⁎ and p values indicating statistical factorial and main effects, respectively, of diet (p<0.05). See Fig. 1 for more details. Data are mean±SEM, n=3.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4488531&req=5

f0030: The effect of diet on the expression of genes in thiol oxidoreductase pathways in cod larvae.The mRNA expression of glutaredoxin (A), thioredoxin-like 1 (B), 4 (C), thioredoxin interacting protein a (D), b (E), thioredoxin reductase 1 (F), 3 (G) and sulfiredoxin 1 (H) in developing cod larvae fed either rotifers (□ red line) or copepods (○ blue line). Shaded areas cover life stages that copepod-fed larvae had elevated growth rates compared to rotifer-fed larvae. Letters indicate statistical relationships between all data points, with ⁎ and p values indicating statistical factorial and main effects, respectively, of diet (p<0.05). See Fig. 1 for more details. Data are mean±SEM, n=3.
Mentions: Over half (57%, Table 1) of the analysed genes coding for proteins that reduce oxidised cysteine residues (thiol oxidoreductases), or interact directly with these enzymes (thioredoxin interacting proteins; txnip’s) were affected by the diets (Fig. 6). Of these, the txnrd’s (1 and 3), txnipa and srxn1 were upregulated, while glrx, txnl1, txnl4 and txnipb were downregulated at either a single development stage or as a main effect in rotifer-fed larvae (Fig. 6). The largest number of differences induced by the diets was observed at development stage 3 (3 out of 5 factorial effects, Fig. 6). Only txnl1 in copepod-fed larvae (Fig. 6B) was dynamically expressed (up then down regulated) during development. With this exception, any changes in gene regulation that occurred in either treatment with development generally persisted until the last stage analysed. For example txnrd3 was upregulated at stage 2 in rotifer fed larvae (p<0.05), from where it remained upregulated until stage 5 (Fig. 6G). The expression of several genes; txnrd1, srxn1 did not change due to development (p>0.05) in either diet group (Fig. 6F and H), or changed late in development, for example txnipa and b in copepod-fed fish, and glrx for both diets (A, D and E).

Bottom Line: The growth and development of marine fish larvae fed copepods is superior to those fed rotifers, but the underlying molecular reasons for this are unclear.The oxidised and reduced glutathione levels, the redox potential, and the mRNA expression of 100 genes in redox system pathways were then compared between treatments during larval development.We found that rotifer/Artemia-fed cod larvae had lower levels of oxidised glutathione, a more reduced redox potential, and altered expression of approximately half of the redox system genes when compared to copepod-fed larvae.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Nutrition and Seafood Research (NIFES), PO Box 2029, NO-5817 Bergen, Norway.

No MeSH data available.