Limits...
Diet affects the redox system in developing Atlantic cod (Gadus morhua) larvae.

Penglase S, Edvardsen RB, Furmanek T, Rønnestad I, Karlsen Ø, van der Meeren T, Hamre K - Redox Biol (2015)

Bottom Line: The growth and development of marine fish larvae fed copepods is superior to those fed rotifers, but the underlying molecular reasons for this are unclear.The oxidised and reduced glutathione levels, the redox potential, and the mRNA expression of 100 genes in redox system pathways were then compared between treatments during larval development.We found that rotifer/Artemia-fed cod larvae had lower levels of oxidised glutathione, a more reduced redox potential, and altered expression of approximately half of the redox system genes when compared to copepod-fed larvae.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Nutrition and Seafood Research (NIFES), PO Box 2029, NO-5817 Bergen, Norway.

No MeSH data available.


The effect of diet on the expression of antioxidant coding genes in cod larvae.The mRNA expression of catalase (A), glutathione peroxidase 1a (B), 3 (C), 4b (D), 7 (E), peroxiredoxin 2 (F), 3 (G), 5 (H), selenoprotein p1a (I) and superoxide dismutase 2 (J) in developing cod larvae fed either rotifers (□ red line) or copepods (○ blue line). Shaded areas cover life stages that copepod-fed larvae had elevated growth rates compared to rotifer-fed larvae. Letters indicate statistical relationships between all data points, with * and p values indicating statistical factorial and main effects, respectively, of diet (p<0.05). See Fig. 1 for more details. Data are mean±SEM, n=3.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4488531&req=5

f0025: The effect of diet on the expression of antioxidant coding genes in cod larvae.The mRNA expression of catalase (A), glutathione peroxidase 1a (B), 3 (C), 4b (D), 7 (E), peroxiredoxin 2 (F), 3 (G), 5 (H), selenoprotein p1a (I) and superoxide dismutase 2 (J) in developing cod larvae fed either rotifers (□ red line) or copepods (○ blue line). Shaded areas cover life stages that copepod-fed larvae had elevated growth rates compared to rotifer-fed larvae. Letters indicate statistical relationships between all data points, with * and p values indicating statistical factorial and main effects, respectively, of diet (p<0.05). See Fig. 1 for more details. Data are mean±SEM, n=3.

Mentions: Around half (53%, Table 1) of the analysed genes from antioxidant pathways were affected by the treatments, but results were mixed in terms of regulation direction, even for closely related genes (Fig. 5). For example, in the glutathione peroxidase family both gpx1 and gpx4b were up-regulated, while gpx7 was down-regulated in rotifer compared to copepod-fed cod larvae (Fig. 5B, C and E); similar contrasts were observed within the peroxiredoxin family (F–H). Much of the diet induced differential regulation of the antioxidant coding genes occurred in the early development stages (i.e. stage 1, sepp1; stage 2, cat, gpx4b, 7, prdx2 and 3, Fig. 5). However several genes, such as gpx3 and prdx3 were differentially regulated at later stages, and thus overall the antioxidant gene family was both an early and persistent responder to dietary differences (Fig. 5). Diet also affected which antioxidant genes were most dynamically expressed during development. For example, up then down-regulation (or vice versa) of gene expression during development occurred for cat, gpx1a, prdx2 in copepod-fed larvae, but for gpx4b and sepp1a in rotifer-fed larvae (Fig. 5).


Diet affects the redox system in developing Atlantic cod (Gadus morhua) larvae.

Penglase S, Edvardsen RB, Furmanek T, Rønnestad I, Karlsen Ø, van der Meeren T, Hamre K - Redox Biol (2015)

The effect of diet on the expression of antioxidant coding genes in cod larvae.The mRNA expression of catalase (A), glutathione peroxidase 1a (B), 3 (C), 4b (D), 7 (E), peroxiredoxin 2 (F), 3 (G), 5 (H), selenoprotein p1a (I) and superoxide dismutase 2 (J) in developing cod larvae fed either rotifers (□ red line) or copepods (○ blue line). Shaded areas cover life stages that copepod-fed larvae had elevated growth rates compared to rotifer-fed larvae. Letters indicate statistical relationships between all data points, with * and p values indicating statistical factorial and main effects, respectively, of diet (p<0.05). See Fig. 1 for more details. Data are mean±SEM, n=3.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4488531&req=5

f0025: The effect of diet on the expression of antioxidant coding genes in cod larvae.The mRNA expression of catalase (A), glutathione peroxidase 1a (B), 3 (C), 4b (D), 7 (E), peroxiredoxin 2 (F), 3 (G), 5 (H), selenoprotein p1a (I) and superoxide dismutase 2 (J) in developing cod larvae fed either rotifers (□ red line) or copepods (○ blue line). Shaded areas cover life stages that copepod-fed larvae had elevated growth rates compared to rotifer-fed larvae. Letters indicate statistical relationships between all data points, with * and p values indicating statistical factorial and main effects, respectively, of diet (p<0.05). See Fig. 1 for more details. Data are mean±SEM, n=3.
Mentions: Around half (53%, Table 1) of the analysed genes from antioxidant pathways were affected by the treatments, but results were mixed in terms of regulation direction, even for closely related genes (Fig. 5). For example, in the glutathione peroxidase family both gpx1 and gpx4b were up-regulated, while gpx7 was down-regulated in rotifer compared to copepod-fed cod larvae (Fig. 5B, C and E); similar contrasts were observed within the peroxiredoxin family (F–H). Much of the diet induced differential regulation of the antioxidant coding genes occurred in the early development stages (i.e. stage 1, sepp1; stage 2, cat, gpx4b, 7, prdx2 and 3, Fig. 5). However several genes, such as gpx3 and prdx3 were differentially regulated at later stages, and thus overall the antioxidant gene family was both an early and persistent responder to dietary differences (Fig. 5). Diet also affected which antioxidant genes were most dynamically expressed during development. For example, up then down-regulation (or vice versa) of gene expression during development occurred for cat, gpx1a, prdx2 in copepod-fed larvae, but for gpx4b and sepp1a in rotifer-fed larvae (Fig. 5).

Bottom Line: The growth and development of marine fish larvae fed copepods is superior to those fed rotifers, but the underlying molecular reasons for this are unclear.The oxidised and reduced glutathione levels, the redox potential, and the mRNA expression of 100 genes in redox system pathways were then compared between treatments during larval development.We found that rotifer/Artemia-fed cod larvae had lower levels of oxidised glutathione, a more reduced redox potential, and altered expression of approximately half of the redox system genes when compared to copepod-fed larvae.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Nutrition and Seafood Research (NIFES), PO Box 2029, NO-5817 Bergen, Norway.

No MeSH data available.