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Diet affects the redox system in developing Atlantic cod (Gadus morhua) larvae.

Penglase S, Edvardsen RB, Furmanek T, Rønnestad I, Karlsen Ø, van der Meeren T, Hamre K - Redox Biol (2015)

Bottom Line: The growth and development of marine fish larvae fed copepods is superior to those fed rotifers, but the underlying molecular reasons for this are unclear.The oxidised and reduced glutathione levels, the redox potential, and the mRNA expression of 100 genes in redox system pathways were then compared between treatments during larval development.We found that rotifer/Artemia-fed cod larvae had lower levels of oxidised glutathione, a more reduced redox potential, and altered expression of approximately half of the redox system genes when compared to copepod-fed larvae.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Nutrition and Seafood Research (NIFES), PO Box 2029, NO-5817 Bergen, Norway.

No MeSH data available.


The effect of diet on gene expression in glutathione synthesis/recycling pathways in cod larvae. The mRNA expression of glutamate–cysteine ligase catalytic subunit (A) and modifier subunit (B), glutathione synthetase (C), glutathione reductase (D), glucose-6-phosphate dehydrogenase a (E) and b (F) genes in developing cod larvae fed either rotifers (□ red line) or copepods (○ blue line). Shaded areas cover life stages that copepod-fed larvae had elevated growth rates compared to those fed rotifers. Letters indicate statistical relationships between all data points, with ⁎ and p values indicating statistical factorial and main effects, respectively, of diet (p<0.05). See Fig. 1 for more details. Data are mean±SEM, n=3.
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f0015: The effect of diet on gene expression in glutathione synthesis/recycling pathways in cod larvae. The mRNA expression of glutamate–cysteine ligase catalytic subunit (A) and modifier subunit (B), glutathione synthetase (C), glutathione reductase (D), glucose-6-phosphate dehydrogenase a (E) and b (F) genes in developing cod larvae fed either rotifers (□ red line) or copepods (○ blue line). Shaded areas cover life stages that copepod-fed larvae had elevated growth rates compared to those fed rotifers. Letters indicate statistical relationships between all data points, with ⁎ and p values indicating statistical factorial and main effects, respectively, of diet (p<0.05). See Fig. 1 for more details. Data are mean±SEM, n=3.

Mentions: All the analysed genes coding for proteins in the GSH synthesis/recycling pathway; gclc, gclm, gss, gsr, g6pda and g6pdb; were upregulated (p<0.05) in rotifer compared to copepod-fed cod larvae at one or more development stages (Fig. 3 and Table 1). In relation to key developmental stages, rotifer-fed cod larvae had stable, while copepod-fed cod larvae had decreasing, expression of gclm and gss (development stages 2–4; Fig. 3B and C). Both treatments had decreased expression of gclc between stages 2 and 4 (Fig. 3A, p<0.05), while development stage did not affect g6pd or gsr gene expression (p>0.05, Fig. 3E F).


Diet affects the redox system in developing Atlantic cod (Gadus morhua) larvae.

Penglase S, Edvardsen RB, Furmanek T, Rønnestad I, Karlsen Ø, van der Meeren T, Hamre K - Redox Biol (2015)

The effect of diet on gene expression in glutathione synthesis/recycling pathways in cod larvae. The mRNA expression of glutamate–cysteine ligase catalytic subunit (A) and modifier subunit (B), glutathione synthetase (C), glutathione reductase (D), glucose-6-phosphate dehydrogenase a (E) and b (F) genes in developing cod larvae fed either rotifers (□ red line) or copepods (○ blue line). Shaded areas cover life stages that copepod-fed larvae had elevated growth rates compared to those fed rotifers. Letters indicate statistical relationships between all data points, with ⁎ and p values indicating statistical factorial and main effects, respectively, of diet (p<0.05). See Fig. 1 for more details. Data are mean±SEM, n=3.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4488531&req=5

f0015: The effect of diet on gene expression in glutathione synthesis/recycling pathways in cod larvae. The mRNA expression of glutamate–cysteine ligase catalytic subunit (A) and modifier subunit (B), glutathione synthetase (C), glutathione reductase (D), glucose-6-phosphate dehydrogenase a (E) and b (F) genes in developing cod larvae fed either rotifers (□ red line) or copepods (○ blue line). Shaded areas cover life stages that copepod-fed larvae had elevated growth rates compared to those fed rotifers. Letters indicate statistical relationships between all data points, with ⁎ and p values indicating statistical factorial and main effects, respectively, of diet (p<0.05). See Fig. 1 for more details. Data are mean±SEM, n=3.
Mentions: All the analysed genes coding for proteins in the GSH synthesis/recycling pathway; gclc, gclm, gss, gsr, g6pda and g6pdb; were upregulated (p<0.05) in rotifer compared to copepod-fed cod larvae at one or more development stages (Fig. 3 and Table 1). In relation to key developmental stages, rotifer-fed cod larvae had stable, while copepod-fed cod larvae had decreasing, expression of gclm and gss (development stages 2–4; Fig. 3B and C). Both treatments had decreased expression of gclc between stages 2 and 4 (Fig. 3A, p<0.05), while development stage did not affect g6pd or gsr gene expression (p>0.05, Fig. 3E F).

Bottom Line: The growth and development of marine fish larvae fed copepods is superior to those fed rotifers, but the underlying molecular reasons for this are unclear.The oxidised and reduced glutathione levels, the redox potential, and the mRNA expression of 100 genes in redox system pathways were then compared between treatments during larval development.We found that rotifer/Artemia-fed cod larvae had lower levels of oxidised glutathione, a more reduced redox potential, and altered expression of approximately half of the redox system genes when compared to copepod-fed larvae.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Nutrition and Seafood Research (NIFES), PO Box 2029, NO-5817 Bergen, Norway.

No MeSH data available.