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In Vivo Osteogenic Potential of Biomimetic Hydroxyapatite/Collagen Microspheres: Comparison with Injectable Cement Pastes.

Cuzmar E, Perez RA, Manzanares MC, Ginebra MP, Franch J - PLoS ONE (2015)

Bottom Line: The osteogenic capacity of biomimetic calcium deficient hydroxyapatite microspheres with and without collagen obtained by emulsification of a calcium phosphate cement paste has been evaluated in an in vivo model, and compared with an injectable calcium phosphate cement with the same composition.Consequently, bone ingrowth was enhanced by the microspheres, with a tenfold increase compared to the cement, which was associated to the higher accessibility for the cells provided by the macroporous network between the microspheres, and the larger surface area available for osteoconduction.No significant differences were found in terms of bone formation associated with the presence of collagen in the materials, although a more extensive erosion of the collagen-containing microspheres was observed.

View Article: PubMed Central - PubMed

Affiliation: Medicine and Animal Surgery Department, Autonomous University of Barcelona (UAB), V Building, 08193 Bellaterra, Spain; Veterinarian Clinical Science Institute, Universidad Austral de Chile. Fundo Teja Norte, Valdivia, Chile.

ABSTRACT
The osteogenic capacity of biomimetic calcium deficient hydroxyapatite microspheres with and without collagen obtained by emulsification of a calcium phosphate cement paste has been evaluated in an in vivo model, and compared with an injectable calcium phosphate cement with the same composition. The materials were implanted into a 5 mm defect in the femur condyle of rabbits, and bone formation was assessed after 1 and 3 months. The histological analysis revealed that the cements presented cellular activity only in the margins of the material, whereas each one of the individual microspheres was covered with osteogenic cells. Consequently, bone ingrowth was enhanced by the microspheres, with a tenfold increase compared to the cement, which was associated to the higher accessibility for the cells provided by the macroporous network between the microspheres, and the larger surface area available for osteoconduction. No significant differences were found in terms of bone formation associated with the presence of collagen in the materials, although a more extensive erosion of the collagen-containing microspheres was observed.

No MeSH data available.


Related in: MedlinePlus

Radiographical assessment of the different samples implanted in the rat femur after 3 month implantation.(A) CPC, (B) coll-CPC, (C) MS and (D) coll-MS. The CPC samples (A,B) clearly showed evidence of material remaining in the site of implantation, whereas the microspheres (C,D) were less visible at the site of implantation.
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pone.0131188.g003: Radiographical assessment of the different samples implanted in the rat femur after 3 month implantation.(A) CPC, (B) coll-CPC, (C) MS and (D) coll-MS. The CPC samples (A,B) clearly showed evidence of material remaining in the site of implantation, whereas the microspheres (C,D) were less visible at the site of implantation.

Mentions: No tendency to particle dispersion was observed intraoperatively by the surgeon during the handling of the microspheres-embebed clot when filling the defect. No clinical evidence of inflammatory response or adverse tissue reaction was found around implants and no toxic signs were observed for the experimental period. One month after implantation, the radiographical assessment showed complete consolidation of the samples in the defect site, with no detectable migration of the material, although the displacement of single microspheres could not be excluded. The radiological images after 3 months are shown in Fig 3. The materials started reducing their radiopacity, especially in the case of the MS (Fig 3C and 3D), with a loss of definition in the defect border as well as a reduction in the initial granular aspect.


In Vivo Osteogenic Potential of Biomimetic Hydroxyapatite/Collagen Microspheres: Comparison with Injectable Cement Pastes.

Cuzmar E, Perez RA, Manzanares MC, Ginebra MP, Franch J - PLoS ONE (2015)

Radiographical assessment of the different samples implanted in the rat femur after 3 month implantation.(A) CPC, (B) coll-CPC, (C) MS and (D) coll-MS. The CPC samples (A,B) clearly showed evidence of material remaining in the site of implantation, whereas the microspheres (C,D) were less visible at the site of implantation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488499&req=5

pone.0131188.g003: Radiographical assessment of the different samples implanted in the rat femur after 3 month implantation.(A) CPC, (B) coll-CPC, (C) MS and (D) coll-MS. The CPC samples (A,B) clearly showed evidence of material remaining in the site of implantation, whereas the microspheres (C,D) were less visible at the site of implantation.
Mentions: No tendency to particle dispersion was observed intraoperatively by the surgeon during the handling of the microspheres-embebed clot when filling the defect. No clinical evidence of inflammatory response or adverse tissue reaction was found around implants and no toxic signs were observed for the experimental period. One month after implantation, the radiographical assessment showed complete consolidation of the samples in the defect site, with no detectable migration of the material, although the displacement of single microspheres could not be excluded. The radiological images after 3 months are shown in Fig 3. The materials started reducing their radiopacity, especially in the case of the MS (Fig 3C and 3D), with a loss of definition in the defect border as well as a reduction in the initial granular aspect.

Bottom Line: The osteogenic capacity of biomimetic calcium deficient hydroxyapatite microspheres with and without collagen obtained by emulsification of a calcium phosphate cement paste has been evaluated in an in vivo model, and compared with an injectable calcium phosphate cement with the same composition.Consequently, bone ingrowth was enhanced by the microspheres, with a tenfold increase compared to the cement, which was associated to the higher accessibility for the cells provided by the macroporous network between the microspheres, and the larger surface area available for osteoconduction.No significant differences were found in terms of bone formation associated with the presence of collagen in the materials, although a more extensive erosion of the collagen-containing microspheres was observed.

View Article: PubMed Central - PubMed

Affiliation: Medicine and Animal Surgery Department, Autonomous University of Barcelona (UAB), V Building, 08193 Bellaterra, Spain; Veterinarian Clinical Science Institute, Universidad Austral de Chile. Fundo Teja Norte, Valdivia, Chile.

ABSTRACT
The osteogenic capacity of biomimetic calcium deficient hydroxyapatite microspheres with and without collagen obtained by emulsification of a calcium phosphate cement paste has been evaluated in an in vivo model, and compared with an injectable calcium phosphate cement with the same composition. The materials were implanted into a 5 mm defect in the femur condyle of rabbits, and bone formation was assessed after 1 and 3 months. The histological analysis revealed that the cements presented cellular activity only in the margins of the material, whereas each one of the individual microspheres was covered with osteogenic cells. Consequently, bone ingrowth was enhanced by the microspheres, with a tenfold increase compared to the cement, which was associated to the higher accessibility for the cells provided by the macroporous network between the microspheres, and the larger surface area available for osteoconduction. No significant differences were found in terms of bone formation associated with the presence of collagen in the materials, although a more extensive erosion of the collagen-containing microspheres was observed.

No MeSH data available.


Related in: MedlinePlus