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In Vivo Osteogenic Potential of Biomimetic Hydroxyapatite/Collagen Microspheres: Comparison with Injectable Cement Pastes.

Cuzmar E, Perez RA, Manzanares MC, Ginebra MP, Franch J - PLoS ONE (2015)

Bottom Line: The osteogenic capacity of biomimetic calcium deficient hydroxyapatite microspheres with and without collagen obtained by emulsification of a calcium phosphate cement paste has been evaluated in an in vivo model, and compared with an injectable calcium phosphate cement with the same composition.Consequently, bone ingrowth was enhanced by the microspheres, with a tenfold increase compared to the cement, which was associated to the higher accessibility for the cells provided by the macroporous network between the microspheres, and the larger surface area available for osteoconduction.No significant differences were found in terms of bone formation associated with the presence of collagen in the materials, although a more extensive erosion of the collagen-containing microspheres was observed.

View Article: PubMed Central - PubMed

Affiliation: Medicine and Animal Surgery Department, Autonomous University of Barcelona (UAB), V Building, 08193 Bellaterra, Spain; Veterinarian Clinical Science Institute, Universidad Austral de Chile. Fundo Teja Norte, Valdivia, Chile.

ABSTRACT
The osteogenic capacity of biomimetic calcium deficient hydroxyapatite microspheres with and without collagen obtained by emulsification of a calcium phosphate cement paste has been evaluated in an in vivo model, and compared with an injectable calcium phosphate cement with the same composition. The materials were implanted into a 5 mm defect in the femur condyle of rabbits, and bone formation was assessed after 1 and 3 months. The histological analysis revealed that the cements presented cellular activity only in the margins of the material, whereas each one of the individual microspheres was covered with osteogenic cells. Consequently, bone ingrowth was enhanced by the microspheres, with a tenfold increase compared to the cement, which was associated to the higher accessibility for the cells provided by the macroporous network between the microspheres, and the larger surface area available for osteoconduction. No significant differences were found in terms of bone formation associated with the presence of collagen in the materials, although a more extensive erosion of the collagen-containing microspheres was observed.

No MeSH data available.


Related in: MedlinePlus

Scanning electron micrographs of the 4 implanted materials.CPC (A-D) and MS (E-H), in the absence (A,C,E,G) or presence (B,D,F,H) of collagen. (C,D,G,H) Microstructural morphology of the calcium deficient hydroxyapatite produced after hydrolysis of the starting α-TCP powder. The pristine CPC (C) and the coll-CPC (D) presented identical morphology, having typical hydroxyapatite crystals. The MS presented similar crystal morphology with bigger crystal domains (G). In the case of coll-MS collagen was exposed in the surface of the microspheres (H).
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pone.0131188.g001: Scanning electron micrographs of the 4 implanted materials.CPC (A-D) and MS (E-H), in the absence (A,C,E,G) or presence (B,D,F,H) of collagen. (C,D,G,H) Microstructural morphology of the calcium deficient hydroxyapatite produced after hydrolysis of the starting α-TCP powder. The pristine CPC (C) and the coll-CPC (D) presented identical morphology, having typical hydroxyapatite crystals. The MS presented similar crystal morphology with bigger crystal domains (G). In the case of coll-MS collagen was exposed in the surface of the microspheres (H).

Mentions: Fig 1 shows the SEM morphology for the different materials studied. Similar microstructures were observed for CPC (Fig 1A and 1C) and coll-CPC (Fig 1B and 1D), with the typical entangled hydroxyapatite crystals (Fig 1C and 1D). In contrast, the addition of collagen introduced significant differences in the morphology of the microspheres obtained from the corresponding ceramic slurries. Whereas the size range of the different microspheres was similar, since in both cases a sieving between 100 and 400 μm was applied, the coll-MS (Fig 1F) exhibited higher sphericity as well as a more homogenous size distribution than the MS (Fig 1E). At higher magnifications, the hydroxyapatite platelets were clearly visible on the surface of the MS (Fig 1G), similar to those observed in the CPC, although bigger in size. In contrast, in the surface of the coll-MS the presence of collagen was visible between the inorganic crystals (Fig 1H).


In Vivo Osteogenic Potential of Biomimetic Hydroxyapatite/Collagen Microspheres: Comparison with Injectable Cement Pastes.

Cuzmar E, Perez RA, Manzanares MC, Ginebra MP, Franch J - PLoS ONE (2015)

Scanning electron micrographs of the 4 implanted materials.CPC (A-D) and MS (E-H), in the absence (A,C,E,G) or presence (B,D,F,H) of collagen. (C,D,G,H) Microstructural morphology of the calcium deficient hydroxyapatite produced after hydrolysis of the starting α-TCP powder. The pristine CPC (C) and the coll-CPC (D) presented identical morphology, having typical hydroxyapatite crystals. The MS presented similar crystal morphology with bigger crystal domains (G). In the case of coll-MS collagen was exposed in the surface of the microspheres (H).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488499&req=5

pone.0131188.g001: Scanning electron micrographs of the 4 implanted materials.CPC (A-D) and MS (E-H), in the absence (A,C,E,G) or presence (B,D,F,H) of collagen. (C,D,G,H) Microstructural morphology of the calcium deficient hydroxyapatite produced after hydrolysis of the starting α-TCP powder. The pristine CPC (C) and the coll-CPC (D) presented identical morphology, having typical hydroxyapatite crystals. The MS presented similar crystal morphology with bigger crystal domains (G). In the case of coll-MS collagen was exposed in the surface of the microspheres (H).
Mentions: Fig 1 shows the SEM morphology for the different materials studied. Similar microstructures were observed for CPC (Fig 1A and 1C) and coll-CPC (Fig 1B and 1D), with the typical entangled hydroxyapatite crystals (Fig 1C and 1D). In contrast, the addition of collagen introduced significant differences in the morphology of the microspheres obtained from the corresponding ceramic slurries. Whereas the size range of the different microspheres was similar, since in both cases a sieving between 100 and 400 μm was applied, the coll-MS (Fig 1F) exhibited higher sphericity as well as a more homogenous size distribution than the MS (Fig 1E). At higher magnifications, the hydroxyapatite platelets were clearly visible on the surface of the MS (Fig 1G), similar to those observed in the CPC, although bigger in size. In contrast, in the surface of the coll-MS the presence of collagen was visible between the inorganic crystals (Fig 1H).

Bottom Line: The osteogenic capacity of biomimetic calcium deficient hydroxyapatite microspheres with and without collagen obtained by emulsification of a calcium phosphate cement paste has been evaluated in an in vivo model, and compared with an injectable calcium phosphate cement with the same composition.Consequently, bone ingrowth was enhanced by the microspheres, with a tenfold increase compared to the cement, which was associated to the higher accessibility for the cells provided by the macroporous network between the microspheres, and the larger surface area available for osteoconduction.No significant differences were found in terms of bone formation associated with the presence of collagen in the materials, although a more extensive erosion of the collagen-containing microspheres was observed.

View Article: PubMed Central - PubMed

Affiliation: Medicine and Animal Surgery Department, Autonomous University of Barcelona (UAB), V Building, 08193 Bellaterra, Spain; Veterinarian Clinical Science Institute, Universidad Austral de Chile. Fundo Teja Norte, Valdivia, Chile.

ABSTRACT
The osteogenic capacity of biomimetic calcium deficient hydroxyapatite microspheres with and without collagen obtained by emulsification of a calcium phosphate cement paste has been evaluated in an in vivo model, and compared with an injectable calcium phosphate cement with the same composition. The materials were implanted into a 5 mm defect in the femur condyle of rabbits, and bone formation was assessed after 1 and 3 months. The histological analysis revealed that the cements presented cellular activity only in the margins of the material, whereas each one of the individual microspheres was covered with osteogenic cells. Consequently, bone ingrowth was enhanced by the microspheres, with a tenfold increase compared to the cement, which was associated to the higher accessibility for the cells provided by the macroporous network between the microspheres, and the larger surface area available for osteoconduction. No significant differences were found in terms of bone formation associated with the presence of collagen in the materials, although a more extensive erosion of the collagen-containing microspheres was observed.

No MeSH data available.


Related in: MedlinePlus