Limits...
Multi-Biomarkers for Early Detection of Type 2 Diabetes, Including 10- and 12-(Z,E)-Hydroxyoctadecadienoic Acids, Insulin, Leptin, and Adiponectin.

Umeno A, Yoshino K, Hashimoto Y, Shichiri M, Kataoka M, Yoshida Y - PLoS ONE (2015)

Bottom Line: We have previously found that fasting plasma levels of totally assessed 10- and 12-(Z,E)-hydroxyoctadecadienoic acid (HODE) correlated well with levels of glycated hemoglobin (HbA1c) and glucose during oral glucose tolerance tests (OGTT); these levels were determined via liquid chromatography-mass spectrometry after reduction and saponification.We then used these groups to evaluate the potential biomarkers for the early detection of type 2 diabetes.Plasma levels of RBP4 and glycoalbumin were higher in Group HN+IGT, compared to those in Group N, and fasting levels of 10- and 12-(Z,E)-HODE/linoleic acids were significantly correlated with levels of RBP4 (p = 0.003, r = 0.380) and glycoalbumin (p = 0.006, r = 0.316).

View Article: PubMed Central - PubMed

Affiliation: Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu, Kagawa 761-0395, Japan.

ABSTRACT
We have previously found that fasting plasma levels of totally assessed 10- and 12-(Z,E)-hydroxyoctadecadienoic acid (HODE) correlated well with levels of glycated hemoglobin (HbA1c) and glucose during oral glucose tolerance tests (OGTT); these levels were determined via liquid chromatography-mass spectrometry after reduction and saponification. However, 10- and 12-(Z,E)-HODE alone cannot perfectly detect early impaired glucose tolerance (IGT) and/or insulin resistance, which ultimately lead to diabetes. In this study, we randomly recruited healthy volunteers (n = 57) who had no known history of any diseases, and who were evaluated using the OGTT, the HODE biomarkers, and several additional proposed biomarkers, including retinol binding protein 4 (RBP4), adiponectin, leptin, insulin, glycoalbumin, and high sensitivity-C-reactive protein. The OGTT revealed that our volunteers included normal individuals (n = 44; Group N), "high-normal" individuals (fasting plasma glucose 100-109 mg/dL) with IGT (n = 11; Group HN+IGT), and diabetic individuals (n = 2; Group D). We then used these groups to evaluate the potential biomarkers for the early detection of type 2 diabetes. Plasma levels of RBP4 and glycoalbumin were higher in Group HN+IGT, compared to those in Group N, and fasting levels of 10- and 12-(Z,E)-HODE/linoleic acids were significantly correlated with levels of RBP4 (p = 0.003, r = 0.380) and glycoalbumin (p = 0.006, r = 0.316). Furthermore, we developed a stepwise multiple linear regression models to predict the individuals' insulin resistance index (the Matsuda Index 3). Fasting plasma levels of 10- and 12-(Z,E)-HODE/linoleic acids, glucose, insulin, and leptin/adiponectin were selected as the explanatory variables for the models. The risks of type 2 diabetes, early IGT, and insulin resistance were perfectly predicted by comparing fasting glucose levels to the estimated Matsuda Index 3 (fasting levels of 10- and 12-(Z,E)-HODE/linoleic acids, insulin, and leptin/adiponectin).

No MeSH data available.


Related in: MedlinePlus

Classification of glucose tolerance using the oral glucose tolerance test.Circle, Group N (normal); square, Group HN+IGT (“high-normal” and impaired glucose tolerance); triangle, Group D (diabetic). Blue, normal insulin resistance; red, borderline insulin resistance; green, insulin resistance determined by homeostasis model assessment of insulin resistance and Matsuda Index 3.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4488492&req=5

pone.0130971.g002: Classification of glucose tolerance using the oral glucose tolerance test.Circle, Group N (normal); square, Group HN+IGT (“high-normal” and impaired glucose tolerance); triangle, Group D (diabetic). Blue, normal insulin resistance; red, borderline insulin resistance; green, insulin resistance determined by homeostasis model assessment of insulin resistance and Matsuda Index 3.

Mentions: The main characteristics and metabolic parameters of the subjects after the 75-g OGTT are shown in Table 1 and Fig 2. Among the 57 volunteers, 44 were characterized as normal (Group N), 11 as “high-normal” (fasting glucose levels of 100–109 mg/dL) with IGT (Group HN+IGT), and 2 as diabetic (Group D). It was surprising that 2 patients with diabetes were included among the volunteers, although the Japan Diabetes Society has reported that approximately 25–40% of “high-normal” subjects develop pre-diabetes and diabetes. Therefore, we considered “high-normal” and IGT subjects as a single group. There were significant differences between Group HN+IGT and Group N in their height and weight, although not in their BMI. The levels of HbA1c, RBP4, glycoalbumin, and hs-CRP tended to increase with reduced glucose tolerance, as indicated by the glucose levels. Fasting levels of RBP4 and glycoalbumin in Group HN+IGT were significantly higher than those from Group N. In contrast, fasting levels of adiponectin, leptin, and insulin in Group HN+IGT were not significantly different from those in Group N.


Multi-Biomarkers for Early Detection of Type 2 Diabetes, Including 10- and 12-(Z,E)-Hydroxyoctadecadienoic Acids, Insulin, Leptin, and Adiponectin.

Umeno A, Yoshino K, Hashimoto Y, Shichiri M, Kataoka M, Yoshida Y - PLoS ONE (2015)

Classification of glucose tolerance using the oral glucose tolerance test.Circle, Group N (normal); square, Group HN+IGT (“high-normal” and impaired glucose tolerance); triangle, Group D (diabetic). Blue, normal insulin resistance; red, borderline insulin resistance; green, insulin resistance determined by homeostasis model assessment of insulin resistance and Matsuda Index 3.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488492&req=5

pone.0130971.g002: Classification of glucose tolerance using the oral glucose tolerance test.Circle, Group N (normal); square, Group HN+IGT (“high-normal” and impaired glucose tolerance); triangle, Group D (diabetic). Blue, normal insulin resistance; red, borderline insulin resistance; green, insulin resistance determined by homeostasis model assessment of insulin resistance and Matsuda Index 3.
Mentions: The main characteristics and metabolic parameters of the subjects after the 75-g OGTT are shown in Table 1 and Fig 2. Among the 57 volunteers, 44 were characterized as normal (Group N), 11 as “high-normal” (fasting glucose levels of 100–109 mg/dL) with IGT (Group HN+IGT), and 2 as diabetic (Group D). It was surprising that 2 patients with diabetes were included among the volunteers, although the Japan Diabetes Society has reported that approximately 25–40% of “high-normal” subjects develop pre-diabetes and diabetes. Therefore, we considered “high-normal” and IGT subjects as a single group. There were significant differences between Group HN+IGT and Group N in their height and weight, although not in their BMI. The levels of HbA1c, RBP4, glycoalbumin, and hs-CRP tended to increase with reduced glucose tolerance, as indicated by the glucose levels. Fasting levels of RBP4 and glycoalbumin in Group HN+IGT were significantly higher than those from Group N. In contrast, fasting levels of adiponectin, leptin, and insulin in Group HN+IGT were not significantly different from those in Group N.

Bottom Line: We have previously found that fasting plasma levels of totally assessed 10- and 12-(Z,E)-hydroxyoctadecadienoic acid (HODE) correlated well with levels of glycated hemoglobin (HbA1c) and glucose during oral glucose tolerance tests (OGTT); these levels were determined via liquid chromatography-mass spectrometry after reduction and saponification.We then used these groups to evaluate the potential biomarkers for the early detection of type 2 diabetes.Plasma levels of RBP4 and glycoalbumin were higher in Group HN+IGT, compared to those in Group N, and fasting levels of 10- and 12-(Z,E)-HODE/linoleic acids were significantly correlated with levels of RBP4 (p = 0.003, r = 0.380) and glycoalbumin (p = 0.006, r = 0.316).

View Article: PubMed Central - PubMed

Affiliation: Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu, Kagawa 761-0395, Japan.

ABSTRACT
We have previously found that fasting plasma levels of totally assessed 10- and 12-(Z,E)-hydroxyoctadecadienoic acid (HODE) correlated well with levels of glycated hemoglobin (HbA1c) and glucose during oral glucose tolerance tests (OGTT); these levels were determined via liquid chromatography-mass spectrometry after reduction and saponification. However, 10- and 12-(Z,E)-HODE alone cannot perfectly detect early impaired glucose tolerance (IGT) and/or insulin resistance, which ultimately lead to diabetes. In this study, we randomly recruited healthy volunteers (n = 57) who had no known history of any diseases, and who were evaluated using the OGTT, the HODE biomarkers, and several additional proposed biomarkers, including retinol binding protein 4 (RBP4), adiponectin, leptin, insulin, glycoalbumin, and high sensitivity-C-reactive protein. The OGTT revealed that our volunteers included normal individuals (n = 44; Group N), "high-normal" individuals (fasting plasma glucose 100-109 mg/dL) with IGT (n = 11; Group HN+IGT), and diabetic individuals (n = 2; Group D). We then used these groups to evaluate the potential biomarkers for the early detection of type 2 diabetes. Plasma levels of RBP4 and glycoalbumin were higher in Group HN+IGT, compared to those in Group N, and fasting levels of 10- and 12-(Z,E)-HODE/linoleic acids were significantly correlated with levels of RBP4 (p = 0.003, r = 0.380) and glycoalbumin (p = 0.006, r = 0.316). Furthermore, we developed a stepwise multiple linear regression models to predict the individuals' insulin resistance index (the Matsuda Index 3). Fasting plasma levels of 10- and 12-(Z,E)-HODE/linoleic acids, glucose, insulin, and leptin/adiponectin were selected as the explanatory variables for the models. The risks of type 2 diabetes, early IGT, and insulin resistance were perfectly predicted by comparing fasting glucose levels to the estimated Matsuda Index 3 (fasting levels of 10- and 12-(Z,E)-HODE/linoleic acids, insulin, and leptin/adiponectin).

No MeSH data available.


Related in: MedlinePlus