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Occurrence and Characterization of Cronobacter spp. in Dehydrated Rice Powder from Chinese Supermarket.

Huang Y, Pang Y, Wang H, Tang Z, Zhou Y, Zhang W, Li X, Tan D, Li J, Lin Y, Liu X, Huang W, Shi Y - PLoS ONE (2015)

Bottom Line: Twenty-three unique fusA alleles and sixty-six PFGE-patterns were detected.All isolated strains were observed to be sensitive or to show intermediate susceptibility to eight tested antimicrobial agents.Identified Cronobacter species, fusA alleles, and subtypes were diverse.

View Article: PubMed Central - PubMed

Affiliation: Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning, Guangxi, China.

ABSTRACT
Cronobacter spp. are emerging food-borne pathogens and have been identified as causative agents of meningitis and necrotizing enterocolitis in infants. Dehydrated rice is popular with a wide range of people and it is frequently used as a substitute for infant milk powder to baby older than four months. The occurrence of Cronobacter spp. was investigated in 1,012 samples of dehydrated rice powder collected from 14 manufacturers in China during 2010 to 2012. The isolates were identified using fusA allele sequencing and subtyped using pulsed-field gel electrophoresis. Seventy-six samples (7.5%) contained Cronobacter spp. The prevalence among manufacturers ranged from 0-28.8%. The 76 isolates included 4 species [Cronobacter sakazakii (52 isolates) Cronobacter malonaticus (14 isolates), Cronobacter dublinensis (7 isolates), and Cronobacter muytjensii (3 isolates)]. Twenty-three unique fusA alleles and sixty-six PFGE-patterns were detected. All isolated strains were observed to be sensitive or to show intermediate susceptibility to eight tested antimicrobial agents. The study revealed serious contamination of dehydrated rice powder by Cronobacter spp., with prevalence varying among manufacturers in China. Identified Cronobacter species, fusA alleles, and subtypes were diverse.

No MeSH data available.


Related in: MedlinePlus

Maximum likelihood tree of the 76 isolated Cronobacter and outgroup species Citrobacter koseri based on the fusA alleles (438 bp) of the Cronobacter multilocus sequence typing dataset.This tree was generated using the MEGA (v. 6.06) with 1000 bootstrap replicates.
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pone.0131053.g001: Maximum likelihood tree of the 76 isolated Cronobacter and outgroup species Citrobacter koseri based on the fusA alleles (438 bp) of the Cronobacter multilocus sequence typing dataset.This tree was generated using the MEGA (v. 6.06) with 1000 bootstrap replicates.

Mentions: The 76 isolates of Cronobacter spp. were identified as four species based on the fusA allele in Joseph’s study [2]: C. sakazakii (52 isolates), C. malonaticus (14 isolates), C. dublinensis (7 isolates), and C. muytjensii (3 isolates) (Fig 1). Eighteen fusA alleles (1, 2, 3, 7, 8, 10, 11, 13, 14, 15, 17, 18, 20, 24, 36, 37, 43, and 67) were identified using the MLST database, five unmatched sequences were sent to MLST manager and generated five new allele fusA sequences (fusA 119 to 123) (Fig 1). A phylogenetic tree was constructed based on the fusA sequences of the 76 isolates, and no isolates were shared among the four Cronobacter species (Fig 1). Seventy-six nearly complete 16S rDNA sequences (~1.5 kb) were obtained, submitted to GenBank database, and assigned accession numbers (Fig 2).


Occurrence and Characterization of Cronobacter spp. in Dehydrated Rice Powder from Chinese Supermarket.

Huang Y, Pang Y, Wang H, Tang Z, Zhou Y, Zhang W, Li X, Tan D, Li J, Lin Y, Liu X, Huang W, Shi Y - PLoS ONE (2015)

Maximum likelihood tree of the 76 isolated Cronobacter and outgroup species Citrobacter koseri based on the fusA alleles (438 bp) of the Cronobacter multilocus sequence typing dataset.This tree was generated using the MEGA (v. 6.06) with 1000 bootstrap replicates.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488472&req=5

pone.0131053.g001: Maximum likelihood tree of the 76 isolated Cronobacter and outgroup species Citrobacter koseri based on the fusA alleles (438 bp) of the Cronobacter multilocus sequence typing dataset.This tree was generated using the MEGA (v. 6.06) with 1000 bootstrap replicates.
Mentions: The 76 isolates of Cronobacter spp. were identified as four species based on the fusA allele in Joseph’s study [2]: C. sakazakii (52 isolates), C. malonaticus (14 isolates), C. dublinensis (7 isolates), and C. muytjensii (3 isolates) (Fig 1). Eighteen fusA alleles (1, 2, 3, 7, 8, 10, 11, 13, 14, 15, 17, 18, 20, 24, 36, 37, 43, and 67) were identified using the MLST database, five unmatched sequences were sent to MLST manager and generated five new allele fusA sequences (fusA 119 to 123) (Fig 1). A phylogenetic tree was constructed based on the fusA sequences of the 76 isolates, and no isolates were shared among the four Cronobacter species (Fig 1). Seventy-six nearly complete 16S rDNA sequences (~1.5 kb) were obtained, submitted to GenBank database, and assigned accession numbers (Fig 2).

Bottom Line: Twenty-three unique fusA alleles and sixty-six PFGE-patterns were detected.All isolated strains were observed to be sensitive or to show intermediate susceptibility to eight tested antimicrobial agents.Identified Cronobacter species, fusA alleles, and subtypes were diverse.

View Article: PubMed Central - PubMed

Affiliation: Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning, Guangxi, China.

ABSTRACT
Cronobacter spp. are emerging food-borne pathogens and have been identified as causative agents of meningitis and necrotizing enterocolitis in infants. Dehydrated rice is popular with a wide range of people and it is frequently used as a substitute for infant milk powder to baby older than four months. The occurrence of Cronobacter spp. was investigated in 1,012 samples of dehydrated rice powder collected from 14 manufacturers in China during 2010 to 2012. The isolates were identified using fusA allele sequencing and subtyped using pulsed-field gel electrophoresis. Seventy-six samples (7.5%) contained Cronobacter spp. The prevalence among manufacturers ranged from 0-28.8%. The 76 isolates included 4 species [Cronobacter sakazakii (52 isolates) Cronobacter malonaticus (14 isolates), Cronobacter dublinensis (7 isolates), and Cronobacter muytjensii (3 isolates)]. Twenty-three unique fusA alleles and sixty-six PFGE-patterns were detected. All isolated strains were observed to be sensitive or to show intermediate susceptibility to eight tested antimicrobial agents. The study revealed serious contamination of dehydrated rice powder by Cronobacter spp., with prevalence varying among manufacturers in China. Identified Cronobacter species, fusA alleles, and subtypes were diverse.

No MeSH data available.


Related in: MedlinePlus