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The effects analysis of two neonicotinoid insecticides on in vitro maturation of porcine oocytes using hanging drop monoculture method.

Ishikawa S, Hiraga K, Hiradate Y, Tanemura K - J. Vet. Med. Sci. (2015)

Bottom Line: We determined the effects of ACE and IMI on the in vitro maturation of porcine oocytes.Significant decreases in nuclear maturation rates were observed in the ACE or IMI-exposed groups.Our results suggest that ACE and IMI exposure was detrimental to porcine oocytes and the extent of the effects depends on the concentration of exposure.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Animal Reproduction and Development, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.

ABSTRACT
Acetamiprid (ACE) and imidacroprid (IMI) are known neonicotinoid insecticides with strong affinities for the insect-selective nicotinic acetylcholine receptor. These provide insect control by hyperstimulating insect nerves and are used for agricultural pest management. However, it has also been reported that ACE and IMI affect mammalian reproductive function. We determined the effects of ACE and IMI on the in vitro maturation of porcine oocytes. Significant decreases in nuclear maturation rates were observed in the ACE or IMI-exposed groups. Also, in matured oocytes from the ACE or IMI-exposed groups, irregular chromosomes were observed. Our results suggest that ACE and IMI exposure was detrimental to porcine oocytes and the extent of the effects depends on the concentration of exposure.

No MeSH data available.


Related in: MedlinePlus

Chromosomes in metaphase II oocytes. (A) control, (B) acetamiprid exposed groups, (C)imidacloprid exposed groups (Orcein staining). Bar=5 µm
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fig_002: Chromosomes in metaphase II oocytes. (A) control, (B) acetamiprid exposed groups, (C)imidacloprid exposed groups (Orcein staining). Bar=5 µm

Mentions: In normal metaphase II oocytes (control group), localized chromosomes of oocyte and polarbody were observed as shown in Fig. 2AFig. 2.


The effects analysis of two neonicotinoid insecticides on in vitro maturation of porcine oocytes using hanging drop monoculture method.

Ishikawa S, Hiraga K, Hiradate Y, Tanemura K - J. Vet. Med. Sci. (2015)

Chromosomes in metaphase II oocytes. (A) control, (B) acetamiprid exposed groups, (C)imidacloprid exposed groups (Orcein staining). Bar=5 µm
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488412&req=5

fig_002: Chromosomes in metaphase II oocytes. (A) control, (B) acetamiprid exposed groups, (C)imidacloprid exposed groups (Orcein staining). Bar=5 µm
Mentions: In normal metaphase II oocytes (control group), localized chromosomes of oocyte and polarbody were observed as shown in Fig. 2AFig. 2.

Bottom Line: We determined the effects of ACE and IMI on the in vitro maturation of porcine oocytes.Significant decreases in nuclear maturation rates were observed in the ACE or IMI-exposed groups.Our results suggest that ACE and IMI exposure was detrimental to porcine oocytes and the extent of the effects depends on the concentration of exposure.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Animal Reproduction and Development, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.

ABSTRACT
Acetamiprid (ACE) and imidacroprid (IMI) are known neonicotinoid insecticides with strong affinities for the insect-selective nicotinic acetylcholine receptor. These provide insect control by hyperstimulating insect nerves and are used for agricultural pest management. However, it has also been reported that ACE and IMI affect mammalian reproductive function. We determined the effects of ACE and IMI on the in vitro maturation of porcine oocytes. Significant decreases in nuclear maturation rates were observed in the ACE or IMI-exposed groups. Also, in matured oocytes from the ACE or IMI-exposed groups, irregular chromosomes were observed. Our results suggest that ACE and IMI exposure was detrimental to porcine oocytes and the extent of the effects depends on the concentration of exposure.

No MeSH data available.


Related in: MedlinePlus