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Characterization of Erysipelothrix rhusiopathiae strains isolated from acute swine erysipelas outbreaks in Eastern China.

Zou Y, Zhu X, Muhammad HM, Jiang P, Li Y - J. Vet. Med. Sci. (2015)

Bottom Line: The 432-bp hypervariable region in spaA gene of the field and attenuated strains were amplified and sequenced.All the field and attenuated strains were highly sensitive to β-lactam antibiotics, tetracyclines and macrolides.So, we can make conclusions that the acute swine erysipelas outbreaks in Eastern China were caused by serotype 1a E. rhusiopathiae strains with different biochemical characteristics, and the virulence of serotype 1a E. rhusiopathiae strains is unrelated with some point mutations in 432-bp hypervariable region of the spaA gene.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Bacteriology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.

ABSTRACT
Recently, a series of acute swine erysipelas outbreaks occurred in Eastern China. Eight strains isolated from cases of septicemia were determined as serotype 1a, and 4 of the isolates were resistant to acriflavine. One isolate strain named HX130709 was attenuated on agar media containing acriflavine dye. The 432-bp hypervariable region in spaA gene of the field and attenuated strains were amplified and sequenced. It was further compared with the vaccine strain G4T10, and thus, the eight field strains can be divided into four spaA-types. The partial spaA gene analysis also showed that no point mutations occurred among different archived passages of HX130709 during the attenuation. Results of pulsed-field gel electrophoresis showed that eight distinct patterns with 22 to 30 DNA fragment bands were produced from field strains, and twelve distinct patterns with 23 to 27 DNA fragment bands were produced from different passages of the attenuated strains. Mouse pathogenicity test showed that the mortality of the mice infected with 10(4) CFU field strains was 100% and the attenuation of strain HX130709 occurred between 46 and 50 passages. All the field and attenuated strains were highly sensitive to β-lactam antibiotics, tetracyclines and macrolides. So, we can make conclusions that the acute swine erysipelas outbreaks in Eastern China were caused by serotype 1a E. rhusiopathiae strains with different biochemical characteristics, and the virulence of serotype 1a E. rhusiopathiae strains is unrelated with some point mutations in 432-bp hypervariable region of the spaA gene.

No MeSH data available.


Related in: MedlinePlus

Pathogenicity of the 8 E. rhusiopathiae field strains towards mice.Each mouse of the untreated control group received a subcutaneous injection of 0.1ml PBS in the right groin and was observed every 12 hr as others.Almost every mouse injected with 104 CFU E. rhusiopathiaefield strains died within 72 hr, while all mice injected with 104 vaccinestrain G4T10 and PBS survived for the end of the experiment.
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fig_004: Pathogenicity of the 8 E. rhusiopathiae field strains towards mice.Each mouse of the untreated control group received a subcutaneous injection of 0.1ml PBS in the right groin and was observed every 12 hr as others.Almost every mouse injected with 104 CFU E. rhusiopathiaefield strains died within 72 hr, while all mice injected with 104 vaccinestrain G4T10 and PBS survived for the end of the experiment.

Mentions: Pathogenicity towards mice: The results of the pathogenicity towards miceare shown in Figs. 3 and 4Fig. 4.


Characterization of Erysipelothrix rhusiopathiae strains isolated from acute swine erysipelas outbreaks in Eastern China.

Zou Y, Zhu X, Muhammad HM, Jiang P, Li Y - J. Vet. Med. Sci. (2015)

Pathogenicity of the 8 E. rhusiopathiae field strains towards mice.Each mouse of the untreated control group received a subcutaneous injection of 0.1ml PBS in the right groin and was observed every 12 hr as others.Almost every mouse injected with 104 CFU E. rhusiopathiaefield strains died within 72 hr, while all mice injected with 104 vaccinestrain G4T10 and PBS survived for the end of the experiment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488401&req=5

fig_004: Pathogenicity of the 8 E. rhusiopathiae field strains towards mice.Each mouse of the untreated control group received a subcutaneous injection of 0.1ml PBS in the right groin and was observed every 12 hr as others.Almost every mouse injected with 104 CFU E. rhusiopathiaefield strains died within 72 hr, while all mice injected with 104 vaccinestrain G4T10 and PBS survived for the end of the experiment.
Mentions: Pathogenicity towards mice: The results of the pathogenicity towards miceare shown in Figs. 3 and 4Fig. 4.

Bottom Line: The 432-bp hypervariable region in spaA gene of the field and attenuated strains were amplified and sequenced.All the field and attenuated strains were highly sensitive to β-lactam antibiotics, tetracyclines and macrolides.So, we can make conclusions that the acute swine erysipelas outbreaks in Eastern China were caused by serotype 1a E. rhusiopathiae strains with different biochemical characteristics, and the virulence of serotype 1a E. rhusiopathiae strains is unrelated with some point mutations in 432-bp hypervariable region of the spaA gene.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Bacteriology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.

ABSTRACT
Recently, a series of acute swine erysipelas outbreaks occurred in Eastern China. Eight strains isolated from cases of septicemia were determined as serotype 1a, and 4 of the isolates were resistant to acriflavine. One isolate strain named HX130709 was attenuated on agar media containing acriflavine dye. The 432-bp hypervariable region in spaA gene of the field and attenuated strains were amplified and sequenced. It was further compared with the vaccine strain G4T10, and thus, the eight field strains can be divided into four spaA-types. The partial spaA gene analysis also showed that no point mutations occurred among different archived passages of HX130709 during the attenuation. Results of pulsed-field gel electrophoresis showed that eight distinct patterns with 22 to 30 DNA fragment bands were produced from field strains, and twelve distinct patterns with 23 to 27 DNA fragment bands were produced from different passages of the attenuated strains. Mouse pathogenicity test showed that the mortality of the mice infected with 10(4) CFU field strains was 100% and the attenuation of strain HX130709 occurred between 46 and 50 passages. All the field and attenuated strains were highly sensitive to β-lactam antibiotics, tetracyclines and macrolides. So, we can make conclusions that the acute swine erysipelas outbreaks in Eastern China were caused by serotype 1a E. rhusiopathiae strains with different biochemical characteristics, and the virulence of serotype 1a E. rhusiopathiae strains is unrelated with some point mutations in 432-bp hypervariable region of the spaA gene.

No MeSH data available.


Related in: MedlinePlus