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Distribution and Inhibition of Liposomes on Staphylococcus aureus and Pseudomonas aeruginosa Biofilm.

Dong D, Thomas N, Thierry B, Vreugde S, Prestidge CA, Wormald PJ - PLoS ONE (2015)

Bottom Line: Staphylococcus aureus and Pseudomonas aeruginosa are major pathogens in chronic rhinosinusitis (CRS) and their biofilms have been associated with poorer postsurgical outcomes.Biofilm growth was inhibited at 24-hour and five-minute exposure time, although the decrease of viability for P. aeruginosa biofilm after liposomal treatment did not reach statistical significance.The distribution and anti-biofilm effects of cationic and anionic liposomes of different sizes differed in S. aureus and P. aeruginosa biofilms.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery- Otorhinolaryngology Head and Neck Surgery, The Queen Elizabeth Hospital, and the University of Adelaide, Adelaide, South Australia; The Rhinology Department, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

ABSTRACT

Background: Staphylococcus aureus and Pseudomonas aeruginosa are major pathogens in chronic rhinosinusitis (CRS) and their biofilms have been associated with poorer postsurgical outcomes. This study investigated the distribution and anti-biofilm effect of cationic (+) and anionic (-) phospholipid liposomes with different sizes (unilamellar and multilamellar vesicle, ULV and MLV respectively) on S. aureus and P. aeruginosa biofilms.

Method: Specific biofilm models for S. aureus ATCC 25923 and P. aeruginosa ATCC 15692 were established. Liposomal distribution was determined by observing SYTO9 stained biofilm exposed to DiI labeled liposomes using confocal scanning laser microscopy, followed by quantitative image analysis. The anti-biofilm efficacy study was carried out by using the alamarBlue assay to test the relative viability of biofilm treated with various liposomes for 24 hours and five minutes.

Results: The smaller ULVs penetrated better than larger MLVs in both S. aureus and P. aeruginosa biofilm. Except that +ULV and -ULV displayed similar distribution in S. aureus biofilm, the cationic liposomes adhered better than their anionic counterparts. Biofilm growth was inhibited at 24-hour and five-minute exposure time, although the decrease of viability for P. aeruginosa biofilm after liposomal treatment did not reach statistical significance.

Conclusion: The distribution and anti-biofilm effects of cationic and anionic liposomes of different sizes differed in S. aureus and P. aeruginosa biofilms. Reducing the liposome size and formulating liposomes as positively charged enhanced the penetration and inhibition of S. aureus and P. aeruginosa biofilms.

No MeSH data available.


Related in: MedlinePlus

Comparison of anti-biofilm effect of liposomes against S. aureus and P. aeruginosa biofilms.Comparison of anti-biofilm effect of liposomes against both S. aureus and P. aeruginosa biofilms tested by alamarBlue assay. The histogram displayed medians with ranges, and the comparison was carried out by Kruskal-Wallis test with Mann-Whitney test for post-hoc. *: P<0.05; **: P<0.01, compared to the untreated control; +MLV: cationic multilamellar vesicle; +ULV: cationic unilamellar vesicle;-MLV: anionic multilamellar vesicle;-ULV: anionic unilamellar vesicle.
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pone.0131806.g006: Comparison of anti-biofilm effect of liposomes against S. aureus and P. aeruginosa biofilms.Comparison of anti-biofilm effect of liposomes against both S. aureus and P. aeruginosa biofilms tested by alamarBlue assay. The histogram displayed medians with ranges, and the comparison was carried out by Kruskal-Wallis test with Mann-Whitney test for post-hoc. *: P<0.05; **: P<0.01, compared to the untreated control; +MLV: cationic multilamellar vesicle; +ULV: cationic unilamellar vesicle;-MLV: anionic multilamellar vesicle;-ULV: anionic unilamellar vesicle.

Mentions: After normalization to the mean of fluorescent intensity for the control wells containing biofilms without liposomal treatment, the result of biofilm relative viability was summarized in Table 3 and Fig 6. The data demonstrated that after five-minute and 24-hour exposure, all tested liposomes inhibited the growth of both S. aureus and P. aeruginosa biofilms. However, the decrease in the viability of P. aeruginosa biofilm after five-minute treatment with the different liposomes did not reach statistical significance. Post-hoc pairwise comparisons showed that at 24-hour exposure, the cationic liposomes displayed significantly stronger anti-biofilm effect than the anionic ones on P. aeruginosa biofilm (+MLV vs–MLV: P = 0.0052, +ULV vs–ULV: P< 0.0001), and the smaller +ULV had stronger anti-biofilm effects than the larger +MLV (P = 0.0249). Although the differences between liposomes against P. aeruginosa biofilm for five minutes and against S. aureus biofilm for both five minutes and 24 hours had no statistical significance (all P>0.05), there was a trend that the anti-biofilm effect of smaller liposomes was stronger than the corresponding larger ones.


Distribution and Inhibition of Liposomes on Staphylococcus aureus and Pseudomonas aeruginosa Biofilm.

Dong D, Thomas N, Thierry B, Vreugde S, Prestidge CA, Wormald PJ - PLoS ONE (2015)

Comparison of anti-biofilm effect of liposomes against S. aureus and P. aeruginosa biofilms.Comparison of anti-biofilm effect of liposomes against both S. aureus and P. aeruginosa biofilms tested by alamarBlue assay. The histogram displayed medians with ranges, and the comparison was carried out by Kruskal-Wallis test with Mann-Whitney test for post-hoc. *: P<0.05; **: P<0.01, compared to the untreated control; +MLV: cationic multilamellar vesicle; +ULV: cationic unilamellar vesicle;-MLV: anionic multilamellar vesicle;-ULV: anionic unilamellar vesicle.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488362&req=5

pone.0131806.g006: Comparison of anti-biofilm effect of liposomes against S. aureus and P. aeruginosa biofilms.Comparison of anti-biofilm effect of liposomes against both S. aureus and P. aeruginosa biofilms tested by alamarBlue assay. The histogram displayed medians with ranges, and the comparison was carried out by Kruskal-Wallis test with Mann-Whitney test for post-hoc. *: P<0.05; **: P<0.01, compared to the untreated control; +MLV: cationic multilamellar vesicle; +ULV: cationic unilamellar vesicle;-MLV: anionic multilamellar vesicle;-ULV: anionic unilamellar vesicle.
Mentions: After normalization to the mean of fluorescent intensity for the control wells containing biofilms without liposomal treatment, the result of biofilm relative viability was summarized in Table 3 and Fig 6. The data demonstrated that after five-minute and 24-hour exposure, all tested liposomes inhibited the growth of both S. aureus and P. aeruginosa biofilms. However, the decrease in the viability of P. aeruginosa biofilm after five-minute treatment with the different liposomes did not reach statistical significance. Post-hoc pairwise comparisons showed that at 24-hour exposure, the cationic liposomes displayed significantly stronger anti-biofilm effect than the anionic ones on P. aeruginosa biofilm (+MLV vs–MLV: P = 0.0052, +ULV vs–ULV: P< 0.0001), and the smaller +ULV had stronger anti-biofilm effects than the larger +MLV (P = 0.0249). Although the differences between liposomes against P. aeruginosa biofilm for five minutes and against S. aureus biofilm for both five minutes and 24 hours had no statistical significance (all P>0.05), there was a trend that the anti-biofilm effect of smaller liposomes was stronger than the corresponding larger ones.

Bottom Line: Staphylococcus aureus and Pseudomonas aeruginosa are major pathogens in chronic rhinosinusitis (CRS) and their biofilms have been associated with poorer postsurgical outcomes.Biofilm growth was inhibited at 24-hour and five-minute exposure time, although the decrease of viability for P. aeruginosa biofilm after liposomal treatment did not reach statistical significance.The distribution and anti-biofilm effects of cationic and anionic liposomes of different sizes differed in S. aureus and P. aeruginosa biofilms.

View Article: PubMed Central - PubMed

Affiliation: Department of Surgery- Otorhinolaryngology Head and Neck Surgery, The Queen Elizabeth Hospital, and the University of Adelaide, Adelaide, South Australia; The Rhinology Department, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

ABSTRACT

Background: Staphylococcus aureus and Pseudomonas aeruginosa are major pathogens in chronic rhinosinusitis (CRS) and their biofilms have been associated with poorer postsurgical outcomes. This study investigated the distribution and anti-biofilm effect of cationic (+) and anionic (-) phospholipid liposomes with different sizes (unilamellar and multilamellar vesicle, ULV and MLV respectively) on S. aureus and P. aeruginosa biofilms.

Method: Specific biofilm models for S. aureus ATCC 25923 and P. aeruginosa ATCC 15692 were established. Liposomal distribution was determined by observing SYTO9 stained biofilm exposed to DiI labeled liposomes using confocal scanning laser microscopy, followed by quantitative image analysis. The anti-biofilm efficacy study was carried out by using the alamarBlue assay to test the relative viability of biofilm treated with various liposomes for 24 hours and five minutes.

Results: The smaller ULVs penetrated better than larger MLVs in both S. aureus and P. aeruginosa biofilm. Except that +ULV and -ULV displayed similar distribution in S. aureus biofilm, the cationic liposomes adhered better than their anionic counterparts. Biofilm growth was inhibited at 24-hour and five-minute exposure time, although the decrease of viability for P. aeruginosa biofilm after liposomal treatment did not reach statistical significance.

Conclusion: The distribution and anti-biofilm effects of cationic and anionic liposomes of different sizes differed in S. aureus and P. aeruginosa biofilms. Reducing the liposome size and formulating liposomes as positively charged enhanced the penetration and inhibition of S. aureus and P. aeruginosa biofilms.

No MeSH data available.


Related in: MedlinePlus