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Identification and Differential Expression of a Candidate Sex Pheromone Receptor in Natural Populations of Spodoptera litura.

Lin X, Zhang Q, Wu Z, Du Y - PLoS ONE (2015)

Bottom Line: It exhibited male-biased expression in the antennae, where they were localized at the base of sensilla trichoidea.Conserved orthologues of these receptors were found amongst known pheromone receptors within the Lepidoptera, and SlituOR3 were placed amongst a clade of candidate pheromone receptors in a phylogeny tree of insect ORs.The changes in the expression level of SlitOR3 and antennal responses after SlitOR3 silencing suggested that SlitOR3 is required for the sex pheromone signaling.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang, China.

ABSTRACT
Olfaction is primarily mediated by highly specific olfactory receptors (ORs), a subfamily of which are the pheromone receptors that play a key role in sexual communication and can contribute to reproductive isolation. Here we cloned and identified an olfactory receptor, SlituOR3 (Genbank NO. JN835270), from Spodoptera litura, to be the candidate pheromone receptor. It exhibited male-biased expression in the antennae, where they were localized at the base of sensilla trichoidea. Conserved orthologues of these receptors were found amongst known pheromone receptors within the Lepidoptera, and SlituOR3 were placed amongst a clade of candidate pheromone receptors in a phylogeny tree of insect ORs. SlituOR3 is required for the EAG responses to both Z9E11-14:OAc and Z9E12-14:OAc SlituOR3 showed differential expression in S. litura populations attracted to traps baited with a series of sex pheromone blends composed of different ratios of (9Z,11E)-tetradecadienyl acetate (Z9E11-14:OAc) and (9Z,12E)-tetradecadienyl acetate (Z9E12-14:OAc). The changes in the expression level of SlitOR3 and antennal responses after SlitOR3 silencing suggested that SlitOR3 is required for the sex pheromone signaling. We infer that variation in transcription levels of olfactory receptors may modulate sex pheromone perception in male moths and could affect both of pest control and monitoring efficiency by pheromone application after long time mass trapping with one particular ratio of blend in the field.

No MeSH data available.


Related in: MedlinePlus

Relative expression of SlituOR3 and the conserved ORco gene in antennae of male S. litura caught in traps baited with different ratios of the conspecific sex pheromone components Z9E11-14:OAc and Z9E12-14:OAc in a tobacco field in Wenzhou, Zhejiang.Expression levels are given relative to the expression of the reference gene SlituRPL8. Duncan’s multiple-range test was used, P<0.05.
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pone.0131407.g007: Relative expression of SlituOR3 and the conserved ORco gene in antennae of male S. litura caught in traps baited with different ratios of the conspecific sex pheromone components Z9E11-14:OAc and Z9E12-14:OAc in a tobacco field in Wenzhou, Zhejiang.Expression levels are given relative to the expression of the reference gene SlituRPL8. Duncan’s multiple-range test was used, P<0.05.

Mentions: We measured the SlituOR3 expression in moths collected from the field population to pheromone-baited traps using qRT-PCR. Expression analysis in male moths attracted revealed that SlituOR3 was differentially expressed in moths attracted by different ratios of the pheromone component (Fig 7). Indeed we also observed some differences in expression of the conserved ORco but its expression levels were more consistent than that of SlituOR3. For SlituOR3 the highest transcription level was in moths trapped by the 4:1 ratio blends of Z9E11-14:OAc and Z9E12-14:OAc, while there was least expression in those caught in traps with a 10:1 blend (Fig 7).


Identification and Differential Expression of a Candidate Sex Pheromone Receptor in Natural Populations of Spodoptera litura.

Lin X, Zhang Q, Wu Z, Du Y - PLoS ONE (2015)

Relative expression of SlituOR3 and the conserved ORco gene in antennae of male S. litura caught in traps baited with different ratios of the conspecific sex pheromone components Z9E11-14:OAc and Z9E12-14:OAc in a tobacco field in Wenzhou, Zhejiang.Expression levels are given relative to the expression of the reference gene SlituRPL8. Duncan’s multiple-range test was used, P<0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4488349&req=5

pone.0131407.g007: Relative expression of SlituOR3 and the conserved ORco gene in antennae of male S. litura caught in traps baited with different ratios of the conspecific sex pheromone components Z9E11-14:OAc and Z9E12-14:OAc in a tobacco field in Wenzhou, Zhejiang.Expression levels are given relative to the expression of the reference gene SlituRPL8. Duncan’s multiple-range test was used, P<0.05.
Mentions: We measured the SlituOR3 expression in moths collected from the field population to pheromone-baited traps using qRT-PCR. Expression analysis in male moths attracted revealed that SlituOR3 was differentially expressed in moths attracted by different ratios of the pheromone component (Fig 7). Indeed we also observed some differences in expression of the conserved ORco but its expression levels were more consistent than that of SlituOR3. For SlituOR3 the highest transcription level was in moths trapped by the 4:1 ratio blends of Z9E11-14:OAc and Z9E12-14:OAc, while there was least expression in those caught in traps with a 10:1 blend (Fig 7).

Bottom Line: It exhibited male-biased expression in the antennae, where they were localized at the base of sensilla trichoidea.Conserved orthologues of these receptors were found amongst known pheromone receptors within the Lepidoptera, and SlituOR3 were placed amongst a clade of candidate pheromone receptors in a phylogeny tree of insect ORs.The changes in the expression level of SlitOR3 and antennal responses after SlitOR3 silencing suggested that SlitOR3 is required for the sex pheromone signaling.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang, China.

ABSTRACT
Olfaction is primarily mediated by highly specific olfactory receptors (ORs), a subfamily of which are the pheromone receptors that play a key role in sexual communication and can contribute to reproductive isolation. Here we cloned and identified an olfactory receptor, SlituOR3 (Genbank NO. JN835270), from Spodoptera litura, to be the candidate pheromone receptor. It exhibited male-biased expression in the antennae, where they were localized at the base of sensilla trichoidea. Conserved orthologues of these receptors were found amongst known pheromone receptors within the Lepidoptera, and SlituOR3 were placed amongst a clade of candidate pheromone receptors in a phylogeny tree of insect ORs. SlituOR3 is required for the EAG responses to both Z9E11-14:OAc and Z9E12-14:OAc SlituOR3 showed differential expression in S. litura populations attracted to traps baited with a series of sex pheromone blends composed of different ratios of (9Z,11E)-tetradecadienyl acetate (Z9E11-14:OAc) and (9Z,12E)-tetradecadienyl acetate (Z9E12-14:OAc). The changes in the expression level of SlitOR3 and antennal responses after SlitOR3 silencing suggested that SlitOR3 is required for the sex pheromone signaling. We infer that variation in transcription levels of olfactory receptors may modulate sex pheromone perception in male moths and could affect both of pest control and monitoring efficiency by pheromone application after long time mass trapping with one particular ratio of blend in the field.

No MeSH data available.


Related in: MedlinePlus